The Role of the Protein Matrix in GFP Chromophore Biosynthesis: A Molecular Dynamics Study

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Current-density functional theory of time-dependent linear response in quantal fluids: recent progress

Vignale and Kohn have recently formulated a local density approximation to the time-dependent linear response of an inhomogeneous electron system in terms of a vector potential for exchange and correlation. The vector potential depends on the induced current density through spectral kernels to be evaluated on the homogeneous electron gas. After a brief review of their theory, the case of inhomogeneous Bose superfluids is considered, with main focus on dynamic Kohn-Sham equations for the condensate in the linear response regime and on quantal generalized hydrodynamic equations in the weak inhomogeneity limit. We also present the results of calculations of the exchange-correlation spectra in both electron and superfluid boson systems

VECTOR FOR THE RELEASE OF AN ACTIVE AGENT WHICH IS PHOTOCLEAVABLE BY IRRADIATION IN THE VISIBLE

A vector for the spatially and temporally controlled release of an active agent comprising nanoparticles of gold coated with a surfactant and an active agent bound to the said surfactant by means of a photocleavable bond, the said vector being capable of releasing the active agent when exposed to light radiation of wavelength greater than 400 nm included in the region of the visible or the region of the near infrared (NIR) through a multiple photon effect. In particular a vector in which the group having a photolabile bond is 1,2,3-triazole, which can for example be obtained by a click chemistry reaction between gold particles on the surface of which peptide molecules having a free carboxyl terminal group and an alkyne side group are chemiadsorbed and an azide of the active agent is described

The Role of Insulin in Tumor Recurrence after Resection of Pancreatic Malignancy. Results of a Prospective Study.

Context Recent findings suggest that insulin levels and insulin resistance are related to the prognosis of patients with different malignancies. Objectives To evaluate the role of insulin in tumor recurrence after resection of pancreatic malignancies. Methods From January 2008 to October 2012, 351 patients with pancreatic malignancy (198 ductal carcinoma, 35 other periampullary adenocarcinoma, 42 neuroendocrine tumors, 45 pancreatic cystic neoplasms and 31 other tumors) underwent resection and were enrolled in a prospective observational study. Clinical and biochemical data (including fasting blood glucose, insulin and C-peptide) were collected. Insulin resistance was evaluated using HOMA2-IR model. Follow-up data were collected one month after discharge and every six months thereafter. Logistic and Cox regression analysis were used when appropriate. Multivariate analysis was performed using variables significant at univariate analysis. Overall survival and disease-free survival were estimated according to Kaplan-Meier. Results Median follow-up time was 764±35 days. Median disease-free survival was 469±29 days and median overall survival 685±59 days. Relapse occurred in 165 of 351 patients (47.0%), 150 of whom died (42.7%). Based on serum insulin levels, patients were divided into tertiles: median survival was 786±145 days, 680±83 days and 520±88 days for first (24.1±9.2 pmol/L), second (50.1±7.8 pmol/L) and third (127±97 pmol/L) tertile, respectively (P=0.042, III vs. I and II pooled together). Cox regression analysis for overall survival showed that older age, higher serum insulin levels (OR=1.024, 95% CI: 1.002-1.047; P=0.032], higher insulin resistance (OR=1.105, 95% CI: 1.003-1.217; 0.043), pN1, G3 and R1 were strongly associated with lower survival. The multivariate analysis confirmed older age and G3 as independent predictors of overall survival. Univariate analysis for predictors of metastatic recurrence found that higher serum insulin levels, diagnosis of ductal carcinoma, pT, pN1, G and R1 were significant factors. Multivariate analysis confirmed that higher insulin level (OR=1.030, 95% CI: 1.001-1.060; P=0.043) and grading (OR=1.656, 95% CI: 1.17-2.3; P=0.004) were independent predictors of metastases. Conclusions Hyper­insulinemia is strongly associated with metastatic recurrence after resection of pancreatic malignancies