Anopheline salivary protein genes and gene families: an evolutionary overview after the whole genome sequence of sixteen Anopheles species

Background: Mosquito saliva is a complex cocktail whose pharmacological properties play an essential role in blood feeding by counteracting host physiological response to tissue injury. Moreover, vector borne pathogens are transmitted to vertebrates and exposed to their immune system in the context of mosquito saliva which, in virtue of its immunomodulatory properties, can modify the local environment at the feeding site and eventually affect pathogen transmission. In addition, the host antibody response to salivary proteins may be used to assess human exposure to mosquito vectors. Even though the role of quite a few mosquito salivary proteins has been clarified in the last decade, we still completely ignore the physiological role of many of them as well as the extent of their involvement in the complex interactions taking place between the mosquito vectors, the pathogens they transmit and the vertebrate host. The recent release of the genomes of 16 Anopheles species offered the opportunity to get insights into function and evolution of salivary protein families in anopheline mosquitoes. Results: Orthologues of fifty three Anopheles gambiae salivary proteins were retrieved and annotated from 18 additional anopheline species belonging to the three subgenera Cellia, Anopheles, and Nyssorhynchus. Our analysis included 824 full-length salivary proteins from 24 different families and allowed the identification of 79 novel salivary genes and re-annotation of 379 wrong predictions. The comparative, structural and phylogenetic analyses yielded an unprecedented view of the anopheline salivary repertoires and of their evolution over 100 million years of anopheline radiation shedding light on mechanisms and evolutionary forces that contributed shaping the anopheline sialomes. Conclusions: We provide here a comprehensive description, classification and evolutionary overview of the main anopheline salivary protein families and identify two novel candidate markers of human exposure to malaria vectors worldwide. This anopheline sialome catalogue, which is easily accessible as hyperlinked spreadsheet, is expected to be useful to the vector biology community and to improve the capacity to gain a deeper understanding of mosquito salivary proteins facilitating their possible exploitation for epidemiological and/or pathogen-vector-host interaction studies

Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in South Europe

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in South Europe C. Antunes1,2, R. Ferro2, R. Ribeiro2, Torres M.C.4, M.J. Velasco4H. García-Mozo4, Galán, C4 , R.Brandao1,3, M.Thibaudon5, R. Albertini6 Ugolotti. M.6, Usberti I.6, Dall’Aglio P.6 and the HIALINE team7 1DInstitute of Mediterranean Agricultural and Environmental Sciences –ICAAM, University of Évora, Portugal 2Department of Chemistry, University of Évora, Portugal 3Department of Biology, University of Évora, Portugal 4Department of Botany, Ecology and Plant Physiology, University of Córdoba 5Réseau National de l Surveillance Aerobiologique, Saint-Genis-l’Argentière, France 6Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Italy 7J.T.M. Buters, Germany, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C.M. Antunes, Portugal, G. Reese, Germany, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy Introduction: Nowadays, pollinosis is affecting a large percentage of population in countries with a western life style. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with an allergen load. Poaceae pollen is recognized as one of the main causes of allergic disease in all Europe. In this study we have tried to compare Poaceae pollen counts in the air and Phl p 5, one of the major allergens of this family, through the use of a high-volume cascade impactor (Chemvol). This study was done in the frame of the European project HIALINE and it compares the results obtained in 2009 by 4 different partners participating in this project: in Córdoba (Spain), Évora (Portugal), Lyon (France) and Parma (Italy). Methodology: Pollen grains were sampled using a 7-day volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm was used for detecting aeroallergens. In each stage polyurethane filters were use as an impacting substrate. Phl p 5 allergen was determined using an allergen specific ELISA. Antibodies for analysis were delivered by Allergopharma Joachim Ganzer KG, the industrial partner in this project. At each location both samplers were placed side-by-side. Results: Most of the allergen was collected in the PM>10µm fraction. Similar profiles between airborne pollen and the total allergenic load was observed during the pollen season. A good correlation was obtained between pollen count and allergen content of the air and a value of 2.5 pg/pollen grain of Poaceae was estimated. Discussion: This is the first year of this project. Nevertheless, results suggest that the allergenic load in outdoor air might be mainly due to pollen bursts. It supports the hypothesis that monitoring the allergens itself in ambient air might be an improvement in allergen exposure assessment. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 2008110

Comparison between Airborne Pollen and Aeroallergen Quantification with the ChemVol Impact Sampler. Olive pollen vs Ole e 1

Comparison between Airborne Pollen and Aeroallergen Quantification with the ChemVol Impact Sampler. Olive pollen vs Ole e 1. Torres M.C.1, C. Antunes2, M.J. Velasco1, R. Ferro2, H. García-Mozo1, R. Ribeiro2, R.Brandao3, Galán, C1 and the HIALINE team4 1Department of Botany, Ecology and Plant Physiology, University of Córdoba 2Department of Chemistry, University of Évora, Portugal 3Department of Biology, University of Évora, Portugal 4J.T.M. Buters, Germany, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C. Antunes, Portugal, G. Reese, Germany, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy Nowadays, pollinosis is affecting a large percentage of population in the countries with a western life style. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with an allergen load. Olive pollen is recognized as one of the main causes of allergic disease in the Mediterranean area. In this study we have tried to compare olive pollen count in the air and Ole e 1 as major allergen of this species, at two different localities in South of Europe: Evora (Portugal) and Córdoba (Spain). At each location both samplers were placed side-by-side. Pollen grains have been sampled using a volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm were used for detecting aeroallergens. Ole e 1 major allergen was determined using allergen specific ELISA´s. Similar behaviour between pollen and the total allergenic load was observed during the pollen season. Nevertheless, at some occasions, during the previous and later period of the pollen season, airborne allergenic load was detected in South Spain, due to the contributions from other Oleaceae species. For this reason the use of these two different methodologies allow a better understanding of the allergenic load in the atmosphere. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 20081107

Produção de mudas de goiabeira.

A goiaba ocupa lugar de destaque dentre as frutas tropicais brasileiras. No Vale do São Francisco, a área plantada com a cultura é de 5.000 hectares, distribuídos entre os estados de Pernambuco e Bahia. A goiaba é uma ótima opção para a diversificação, entretanto, com os problemas surgidos por causa do nematoide, muitos pomares foram erradicados. Visando contribuir para a redução desses problemas, deve-se dar importância especial à produção de mudas livres de nematoides, pois, é no preparo delas que grande parte das áreas são contaminadas por essa praga. A produção de mudas é uma das etapas fundamentais no processo de implantação de um pomar e pode ser determinante para o sucesso da cultura a ser implantada. As técnicas de produção devem atender às necessidades do produtor, levando-se em consideração a disponibilidade e a localização de áreas, o grau de tecnologia e os recursos financeiros disponíveis.bitstream/item/54791/1/COT148.pd

Biorreatores: aspectos gerais e sua utilização para cultura de tecidos vegetais.

Cultura de Tecidos Vegetais; Biorreatores na cultura de tecidos vegetais; Vantagens do uso de biorreatores em relação ao processo de micropropagação convencional; Problemas no uso de biorreatores e como contorná-los; Principais tipos de biorreatores; Principais parâmetros físicoquímicos em um biorreator; Formas e efeitos da agitação; Formas de transferência e efeitos do oxigênio dissolvido; Efeitos provocados pelas variações de CO2 e pH.bitstream/CPATSA-2009-09/40172/1/SDC214.pd

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in SW Europe

The European project HIALINE: Comparison between Poaceae Airborne Pollen Counts and Phl p5 Aeroallergen Quantification in SW Europe C. Antunes1*, R. Ferro1, R. Ribeiro1, Torres M.C.3, M.J. Velasco3H. García-Mozo3, Galán, C3 , R.Brandao2, M.Thibaudon4 and the HIALINE team5 1Department of Chemistry, University of Évora, Portugal 2Department of Biology, University of Évora, Portugal 3Department of Botany, Ecology and Plant Physiology, University of Córdoba 4Réseau National de l Surveillance Aerobiologique, Saint-Genis-l’Argentière, France 5J.Buters, Germanny, M. Thibaudon, France, M. Smith, Great Britain, C. Galan, Spain, R. Brandao and C.M. Antunes, Portugal, R. Albertini, Italy, L. Grewling, Poland, A. Rantio-Lehtimäki, Finland, S. Jäger and U. Berger, Austria, I. Sauliene, Lithuania, L. Cecchi, Italy * Presenting Author: Antunes, C. Department of Chemistry, University of Évora, Portugal. Phone +351 266745319 ; email: [email protected] Introduction: Nowadays, pollinosis is affecting in a large percentage of population in the developed countries. The existence of allergenic activity in the atmosphere is not only associated to pollen grains and fungal spores, but also to submicronic and paucimicronic biological particles. The origin of these allergens can be due to the rupture of pollen transported in the atmosphere or to the presence of allergens from other parts of the plant making amorphous material with allergenic load. Poaceae pollen is recognized as one of the main causes of allergic disease in all Europe. In this study we have tried to compare Poaceae pollen counts in the air and Phl p 5, one of the major allergens of this family, through the use of a high-volume cascade impactor (Chemvol). This study was done in the frame of the European project HIALINE and it compares the results obtained in 2009 by 3 different partners participating in this project: in Córdoba (Spain), Évora (Portugal) and Lyon (France). Methodology: Pollen grains have been taken out using a 7-day volumetric Hirst type spore trap. Chemvol high-volume cascade impactor equipped with stages PM>10µm, 10 µm>PM>2.5µm were used for detecting aeroallergens. In each stage polyurethane filters were applied. Phl p5 allergen was determined using allergen specific ELISA´s. Antibodies for analysis are delivered by Allergopharma Joachim Ganzer KG, the industrial partner in this project. Both samplers were placed side-by-side. Results: Most of the allergen was collected in the PM>10µm fraction. Similar profiles between airborne pollen and the total allergenic load was observed during the pollen season. A good correlation was obtained and a value of 2.5 pg/pollen grain of Poaceae was estimated. Discussion: These results suggest that the allergenic load in outdoor air might be mainly due to pollen bursts. It supports the hypothesis that monitoring the allergens itself in ambient air might be an improvement in allergen exposure assessment. This work was supported in part by the European Agency for Health and Consumers EAHC, Luxembourg, under the grant agreement 2008110