Optimization of Hydrogen Production from Pickle Bamboo Shoot Wastewater by Rhodopseudomonas palustris TN1

A pickle bamboo shoot is a top marketing product in the East of Thailand, especially Prachinburi province. Vinegar (acetic acid) and sea salt (NaCl) are added during pickle bamboo shoot productions to preserve the color and texture. These high acidity (pH 3-4), high salt concentration (6.0%) and dark-brown color of the pickle bamboo shoot wastewater (PBSW) lead to the difficulty for bioremediation of PBSW treatment. Rhodopseudomonas palustris TN1 was capable of surviving in 3.0% (w v-1) NaCl medium and produce hydrogen under anaerobic-light condition. The Aim of this study is to investigate the ability of biohydrogen production in the PBSW by TN1, the effect of initial pH (6.5, 7.0 and 7.5) and light intensities (1,000, 3,000 and 5,000 lux) were performed under anaerobic-light condition. The hydrogen content in biogas using wastewater as the medium was found to be 98.51%. The optimal condition for the highest hydrogen production was pH 7.36 and light intensity of 3,200 lux at room temperature (30±2ºC) giving hydrogen 73.57±5.29 mL L-1 and dry cell weight (DCW) of 5.36±0.51 g L-1 within 96 hrs. In conclusion, TN1 can consume the PBSW as a carbon source to produce hydrogen

Kinetic Analysis of Xylose Production from Palm Pressed Fiber by Sulfuric Acid

Palm pressed fiber (PPF) is lignocellulosic materials containing xylose and glucose. These high sugars contained in PPF can be used as a substrate for ethanol production by microorganisms. The PPF was delignified using sodium hypochlorite as a pretreatment for sugars production. By using mathematic model, 30.6 g L-1 of xylose and 2.2 g L-1 of glucose was obtained under the optimum condition of 5% (v.v-1) H2SO4 at 120 ºC for 30 min. At this condition, acetic acid and furfural concentrations were produced at 7.2 g L-1 and 0.9 g L-1, respectively. Kinetic parameters were also investigated in order to predict concentrations of xylose, glucose, acetic acid and furfural in the delignified PPF hydrolysate. Yield of xylose was 85.74% of the initial xylose content

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Abstract Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extract- malt (YM) medium at 30 \ub1 2\ubaC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L-1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L-1, ethanol yield 0.47 g g-1) than those of free cells (ethanol production 10.8 g L-1, ethanol yield 0.44 g g-1) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843

Assessment of Probiotic Properties in Lactic Acid Bacteria Isolated from Fermented Vegetables

The present study was conducted in order to screen lactic acid bacteria which have the probiotic properties. A total of 82 isolates of lactic acid bacteria was preliminarily screened from fermented vegetables. Most strains exhibited autoaggregation ability, cell surface hydrophobicity and antimicrobial activity against food-borne pathogens Escherichia coli O157:H7 DMST 12743 and Salmonella Typhimurium ATCC 13311. The results revealed that 23 isolates possess some desirable probiotic properties and selected to determine other probiotic properties including antibiotic resistance, coaggregation ability with E. coli O157:H7 DMST 12743 and S. Typhimurium ATCC 13311, heat tolerance at 65°C for 60 min and the ability to survive under gastrointestinal tract condition pH 2.0 and 8.0. From probiotic properties determination mentioned above, it was observed that the strain KMUTNB 5-9, KMUTNB 5-36 and KMUTNB 6-21 were found to meet all the criteria and could be considered as potential probiotic. The identification of the strains based on 16S rDNA sequencing analysis indicated that the strain KMUTNB 5-9, KMUTNB 5-36 and KMUTNB 6-21 were identified to be Pediococcus pentosaceus

การผลิตไบโอเอทานอลและเฟอร์ฟูรอลจากเส้นใยปาล์ม

Thesis (Ph.D. Biotechnology)--Prince of Songkla University, 201

Microbial diversity analysis of thermophilic hydrogen-producing consortia from hot spring in the south of Thailand and evaluate cashew apple juice as a substrate

This study aims to develop thermophilic hydrogen-producing consortia from ten sediment samples of hot springs in southern Thailand by repeated batch cultivation at 60°C with fructose, glucose and combination of fructose and glucose. The promising hydrogen production potentials consortia were obtained from thermophilic enriched cultures and encoded as NST1 consortia. NST1 consortia exhibited the maximum hydrogen yields of 259 ml/g sugar consumed when fructose-glucose mixed was used as a substrate. PCR amplified 16S rDNA product was separated by using denaturing gradient gel electrophoresis (DGGE) to identify the diversity of bacteria in NST1 consortia. The phyologenetic profile of the NST1 consortia showed a significant diversity in the microbial community, where major nucleotide sequences were affiliated to class Clostridia, followed by Bacteroidetes, Deltaproteobacteria, and Flavobacteria. Time course of hydrogen production revealed that NST1 consortia gave the maximum hydrogen yield of 540 ml H2 /g sugar consumed after 96 hrs of cultivation when 60% of cashew apple juice was used as a substrate

Response Surface Optimization for Antioxidant Extraction and Attributes Liking from Roasted Rice Germ Flavored Herbal Tea

The optimal process conditions when examining the antioxidant potential, total polyphenol content, and attribute liking in roasted rice germ flavored herbal tea were investigated using response surface methodology (RSM). The influence upon the extraction process of time and temperature was assessed using a full factorial design on three levels with two variables (32), involving five central point replicates. Extraction temperature (70 °C, 80 °C, and 90 °C) and extraction time (3 min, 4.5 min, and 6 min) served as independent variables, while the dependent variables were allocated to the regression equation to determine antioxidant activity (R2 = 0.941) along with total polyphenol content (R2 = 0.849), flavor liking score (R2 = 0.758), and overall liking score (R2 = 0.816). Following experimentation, it was determined that the optimal time and temperature conditions to maximize total polyphenol content, antioxidant activity, flavor, and overall liking score were in a range of 86 °C to 90 °C for 3.4 min to 5.9 min. When these conditions were imposed, the antioxidant potential, total polyphenol content, flavor, and overall liking score were >70% for DPPH scavenging activity, >75 mgGAE/g, >6.7 (like moderately), and >6.5 (like moderately), respectively