Gait analysis.

<p>Gait speed (m/sec) was measured at week 8. There was a significant main effect of movement restriction on gait speed <i>(ANOVA p</i> = .03), but no significant effect of injury (ANOVA <i>p = 2</i>.<i>56)</i>.</p

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Salivary cortisol measurement

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Ultrasound shear strain measurement

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Pig Weight

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Spinal cord substance P and CGRP measurement

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Gait measurement

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Ultrasound Thickness measurement

Ultrasound measurements of tissue thickness.

<p>The tissue thickness in four Zones (See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0147393#pone.0147393.g003" target="_blank">Fig 3</a> for Zone locations) was measured at the L3-4 vertebral level on the non-intervention side. There was no significant difference in the combined thickness of dermis and superficial connective tissue (Zone 1) among groups (<i>p = 0</i>.<i>60)</i>. The thickness of Zone 2 (deep subcutaneous tissue and perimuscular fascia), Zone 3 (deep subcutaneous tissue) and Zone 4 (perimuscular fascia) all were significantly greater in the injured pigs compared with the other groups (ANOVA, main effect of injury for p = .007 (Zone 2), <i>p</i> = .026 (Zone 3) <i>p</i> = .04 (Zone 4)).</p

Pig growth over the course of the experiment.

<p>There were no significant weight differences among groups at 0, 5, and 8 weeks.</p

Location of tissue zones used for measurement of tissue thickness in ultrasound images.

<p>D: dermis, SST: superficial subcutaneous tissue, DST: deep subcutaneous tissue, TF: thoracolumbar fascia.</p