Duchenne muscular dystrophy (DMD) protein- protein interaction mapping

ObjectiveDuchenne muscular dystrophy as one of the mortal diseases is prominent to study in terms of molecular investigation. In this study, the protein interaction map of this muscle-wasting condition is generated to gain a better knowledge of interactome profile of DMD.Materials & Methods Applying Cytoscape and String Database, the protein-protein interaction network was constructed and the gene ontology of the constructed network was analyzed for biological process, molecular function, and cell component annotations.ResultsThe results indicate that among 100 proteins that are related to DMD, Dystrophin, Utrophin, Caveolin 3, and Myogenic differentiation 1 play key roles in DMD network. In addition, the gene ontology analysis showed that regulation processes, kinase activity and sarcoplasmic reticulum are the highlighted biological processes, molecular function, and cell component enrichments respectively for the proteins related to DMD.  ConclusionIn conclusion, the central proteins and the enriched ontologies can be suggested as possible prominent agents in DMD; however, the validation studies may be required

Interaction network prediction and analysis of Anorexia Nervosa

ObjectivesAnorexia Nervosa (AN) as a mental condition is a common eating disorder among young women. This study aims to shed lights on molecular behavior of this serious disorder in terms of protein interacting profile to provide further insight about its complexity.Materials & MethodsThe AN related genes were extracted from STRING database and included in interactome via Cytoscape software. The central nodes of the network were enriched via gene ontology (GO) by ClueGO+CluePedia and the action relationship between the nodes were determined by CluePedia.ResultsSix genes including LEP, INS, POMC, GCG, SST, and ALB were introduced as hub-bottlenecks that among them LEP, INS, and POMC were the super hub-bottlenecks based on further analysis. Action map analysis showed prominent role of hubs relative to bottlenecks in the network. Regulation of behavior, regulation of carbohydrate biosynthetic process, and regulation of appetite are the top associated processes for the identified hub genes.ConclusionThe topological analysis proposed the five hub-bottlenecks as the most central genes in the network, these genes and their contributing biological terms may suggest additional importance in AN pathogenesis and thereby possible candidates for therapeutic usage.  However, further studies is required to justify these findings.

A Review of Various Methods of Management of Risk in the Field of Emergency Medicine

BACKGROUND: The main concept of risk management in the emergency department (ED) contains a broader meaning, so that; it's known as a sudden event or situation which would happen at an uncertain future that has some negative or positive impacts which could be called threat or opportunity respectively. However, the knowledge of risk management could cover the overall procedures involved with administering the planning of risk management, identification, investigation, monitoring and also step by step clinical examination. One of the main tools for preventing adversities is evaluating and management of possible risks. AIM: One of the main objectives of the present study is recognising the most frequent types of the risk happening in the EDs. Moreover, the present study is trying to evaluate the possible risks which could happen among various ED sections. METHODS: Six databases of EMBASE, HubMed, Cochrane Library, MEDLINE, PubMed, CHBD and Goggle scholar were chosen for discovering much-related articles from the year 2005 to 2019. A total number of 68 were chosen finally to be reviewed more precisely based on the main objective of the present study. RESULTS: Precise planning, preparing sufficiently and conducting the process of continuous monitoring are needed for ensuring the fact that any possible risks could be managed through these planned strategies. On the other hand, by modifying the patients' beliefs, anticipations and the available social culture about the importance of risk management issue, the overall objective of the present study could be achieved at higher rates. CONCLUSION: Moreover, because the potential of occurrence of risk in EDs is high and approximately more than half of them are fatal, more precise adequate systematic plans for management of them should result

Evaluation of skin response after erbium:yttrium–aluminum–garnet laser irradiation: a network analysis approach

Introduction: Application of laser in medicine and cosmetic purposes has raised grossly in recent years. There are contradictory finding about its side effects. In this research critical differentially expressed proteins after irradiation erbium:yttrium–aluminum–garnet (Er:YAG) laser on skin are investigated.Methods: Proteome data including 31 proteins were obtained from a proteomics investigation of laser irradiation, Er:YAG on female mouse skin that are published by Pan et al. The query proteins and 100 related ones were included in the protein-protein interaction (PPI) network. The central nodes were determined and all of nodes were included in action maps. Expression, activation, inhibition, binding, and reaction were considered in action plan.Results: Numbers of 16 proteins were recognized by STRING database and were included in the network. Except PHRF1, the other 15 query proteins were included in the main connected component of the constructed network. Ten central nodes of the network and ten numbers of top query proteins based on degree value were identified as central proteins of the network. All nodes of the network analyzed via action maps and the important acted nodes were determined as RPSA, GAPDH, TPT1, DCTN2, HSPB1, and PDIA3.Conclusion: Two balanced processes including cancer promotion and cancer prevention were after irradiation were identified

Protein interaction mapping related to Becker Muscular Dystrophy

ObjectiveBecker Muscular Dystrophy (BMD) is a neuromuscular disorder which is incurable. In this research protein interaction network of most associated proteins with Becker muscular dystrophy to provide a better clarification of disorder underlying mechanism is investigated.Materials & Methods The related genes to BMD were retrieved via string data base and conducted by Cytoscape and the related algorithms. The network centrality analysis was performed based on degree, betweennes, closeness and stress parameters. Gene ontology and clustering were performed via ClueGO analysis.ResultsThe results indicate that DMD as the super-hub as well as other central proteins including UTRN, TTN, DNM2 and RYR1 are important in BMD in terms of interaction features. The impairment of muscular contraction may be vital in BMD disease pathogenesis as it is the highlighted biological process term obtained by ClueGO analysis.ConclusionIt is possible that DMD targeting may be the main concern for dystrophy clinical approaches. However the other suggested proteins should be evaluated.  In conclusion, targeting these key proteins are required for treatment goals following extensive validation studies

Assessment of Dysregulation of HERC6 and Essential Biological Processes in Response to Laser Therapy of Human Arm Skin

Introduction: The widespread application of lasers in medicine, especially in the treatment of diseases implies more investigations to understand the precious molecular mechanism of the laser effect on the human body. In the present study, the prominent role of HERC6 in response to CO2 Laser therapy of human skin is investigated.Methods: The numbers of 16 gene expression profiles before and after the treatment with the CO2 laser are downloaded from Gene Expression Omnibus (GEO), and differentially-expressed genes (DEGs) are analyzed to find the significant DEGs. Gene ontology analysis revealed that HERC6 and a set of its neighbors played a significant role in response to laser application.Results: The expression changes of 52 significant DEGs were compared via heat map analysis and 27 significant DEGs were introduced as the critical genes which are involved in response to laser irradiation. “Thymidylate kinase activity” among 9 clusters of biological terms was highlighted as an important biological process related to the identified DEGs. HERC2 was proposed as a critical DEG which was related to several essential cellular processes in response to laser application.Conclusion: The findings from the present study indicate that HERC6 and the numbers of its first neighbors are involved in the essential cellular response to laser therapy of human skin

The study of fibroblast proteome changes in the presence of Ethanol

    Ethanol known as ethyl alcohol is being widely used around the world. Many serious diseases are related with its consumption.  Alcohol posses many divers effects on human body including risk of cirrhosis and/or hepatocellular carcinoma. Therefore, analysis of this component is prominent. Fibroblast cells were cultured in various dosages of ethanol. The effective dosage was then investigated by proteomic methods.  Separated proteins of fibroblast cells by Two-Dimensional Gel (2DG) Electrophoresis method based on pI and MW were analyzed based on spots alteration by Same Spot Software. Furthered analysis was carried out with vigorous statistical analysis based on significant folding changes and one-way ANOVA. About 372 protein spots were identified and among them 65 of them were having significant expression profile, which is evaluated as p <0.05. Therefore, ethanol can induce a great impact on protein profile of fibroblast. It is concluded that altering morphologic features and viability, as well as protein expression changes, confirm toxic properties of ethanol in human body.

Evaluation of Laser Effects on the Human Body After Laser Therapy

Lasers have wide applications in the treatment and diagnosis of diseases and various medical fields. Laser therapy like the other methods has advantages and disadvantages. Some risks such as bleeding, pain, and infection are created after laser therapy. Explanation and evaluation of laser effects on cell function, tissue, and the body are the aims of this study. We reviewed papers available from 1986 to 2019 about the effects of lasers on cells and tissue. An online search of PubMed, Science Direct and Google scholar using such keywords as “laser”, “cell”, “tissue”, “body” and “side effects” was performed. The laser photons are absorbed by chromophores, resulting in the target heating and localized damage. Laser irradiation alters cellular metabolism and cellular functions. These alterations may be accompanied by undesired side effects which can be monitored via metabolites level change in the body. Based on this finding, laser therapy may be associated with several side effects and complications; therefore, before treatment, the determination of laser types and their properties is necessary to avoid creating side effects. The advantages and disadvantages of the treatment type should be considered in order to choose the best treatment with the least side effects. The patients’ awareness of possible side effects before treatment and also an effective follow-up and management of patients after action are two important points in laser therapy. Training curriculum definition should be determined for laser applicant qualifications in different medical fields

Application of Proteomics in Cancer Study

Cancer is one of the most malignant diseases in the world, accounting for 7.6 million deaths (around 13% of all deaths) in 2008 based on WHO reports. Early detection of cancer is vital due to its final control and prevention. Despite advances in diagnostic strategies, they have not the required sensitivity and specificity for prognosis. During the last decays, one of the most challenges for cancer research is to determine biological basis of this malignancy as a characteristic agents for an early-stage cancer. Understanding these agents requires molecular level examination of the disease followed by analysis of protein networks and their interactions in cells, signaling events among cancer cells, interactions among the cancer cells, and the tumor microenvironment. Proteomics as one of the modern areas of biochemistry holds great promise in cancer study. Inasmuch as, proteome reflects the real state of a cell, tissue or organism, it is expected to achieve more accurate tumor markers for disease diagnosis and therapeutic monitoring. In fact, the utility of this innovative large-scale proteome analyzer has shown significant prospective in biomarker discovery, patient monitoring, drug targeting and cell signaling; moreover, advances in the field of proteomics will provide new insight into the molecular complexity of the disease process, and enable the development of tools to help in treatment as well as in detection and prevention. In this review, proteomics approaches in cancer studies have been represented and discussed

The Evaluation of Laser Application in Surgery: A Review Article

There are several types of surgeries which use lasers in the operating room. Surgeons use lasers in general surgery or surgical specialties to cut, coagulate, and remove tissue. In modern medicine, the application of laser therapy is an attractive subject due to its minimal invasive effect. Today lasers are widely used in the treatment and diagnosis of many diseases such as various cancers, lithotripsy, ophthalmology, as well as dermatology and beauty procedures. Depending on the type of lasers, the wavelength and the delivery system, most lasers have replaced conventional surgical instruments for better wound healing results. Over time, by using many different tools and devices, new lasers have been created; as a result, they are used in a wide range of medical special cases. In this review, laser applications in surgery and its beneficial effects compared to previous surgeries with the aim of providing appropriate therapeutic and non-invasive solutions with minimal side effects after surgery are investigated