Future vital prospect of gene expression factors of lef-7 (baculovirus expression): Old body, young cherub

BackgroundBaculovirus; late expression factors (Lef-7) have potential roles for protein expression in insect and mammalian cells; Efficient expression of recombinant proteins to facilitate the practical and structural investigation.AimsLef-7 might play crucial roles in transcription and translation reactions of insect cell lines.Methods Materials and Methods: All required information regards Lef-7 was generated by exploring the internet search engine like as (PubMed, Wiley, ScienceDirect, CNKI, ACS, Google Scholar, Web of Science, SciFinder, and Baidu Scholar) and libraries.ResultsThese properties issue crucial scope for DNA cloning and act as a vital vector for insect and mammalian cells. Left-7 could be the significant site in the development of the vaccine for a couple of chronic diseases. Further investigation needs to study on therapeutic vaccines with few immunologic advantages over proteins derived from mammalian sources, and animal sources. Lef-7 demonstrates the significant impact in the fields of DNA immunology research to insight into the mechanistic and utilitarian link between autoimmunity, infectious diseases, and cancer.ConclusionThis review reveals Lef-7 gene function offers a workable strategy for the expression of whole viral protomers as the future prospect of Lef-7

Evaluation of Potential Antioxidant Activity of Leaves of Bauhinia Acuminate

Abstract The methanol extracts of leaves of Bauhinia acuminata (MESF) and their different fractions obtained fro

Histone deacetylase (HDAC) inhibitor curcumin upregulates mitochondrial uncoupling protein1 (UCP1) and mitochondrial function in brown adipocytes, in-silico study and screening natural drug library

Mitochondrial dysfunction has been associated with diverse pathological conditions globally. Specifically, in adipose tissues, mitochondrial dysfunction is the primary cause of obesity and obesity-related illnesses. An existing drugs such as atorvastatin and other lipid-lowering drugs demonstrated adverse effects and initiated other diseases. Thus, we need to explore new methods to prevent and treat obesity. In this study, we used the cell screening method to identify several natural compounds that increase adipocyte UCP1 gene expression. The identified drug Curcumin was evaluated in cell models and the In-silico model. We found curcumin is an active compound of turmeric belonging to Zingiberaceae (ginger family), which activates the Nrf2 mechanism. Curcumin potentially endorses the expression of UCP1 in the brown adipocyte in vitro cellular model. Curcumin plays an important role that modulating mitochondrial function and improving mitochondrial DNA quantification, ATP production, and cell viability. We have established an efficient in vitro cell experiment system to study the metabolic regulation of UCP1. The in-silico model revealed curcumin-UCP1 interaction. Curcumin, via enhancing mitochondrial activity, could be a helpful therapeutic molecule against metabolic disorders or obesity-related diseases. Curcumin will be the subject of more research in both human and murine models, which will provide novel therapeutic pathways for the treatment of metabolic illnesses by modulating the control of mitochondrial function

The Integrated Bioinformatic Approach Reveals the Prognostic Significance of LRP1 Expression in Ovarian Cancer

A hyperactive tumour microenvironment (TME) drives unrestricted cancer cell survival, drug resistance, and metastasis in ovarian carcinoma (OC). However, therapeutic targets within the TME for OC remain elusive, and efficient methods to quantify TME activity are still limited. Herein, we employed an integrated bioinformatics approach to determine which immune-related genes (IRGs) modulate the TME and further assess their potential theragnostic (therapeutic + diagnostic) significance in OC progression. Using a robust approach, we developed a predictive risk model to retrospectively examine the clinicopathological parameters of OC patients from The Cancer Genome Atlas (TCGA) database. The validity of the prognostic model was confirmed with data from the International Cancer Genome Consortium (ICGC) cohort. Our approach identified nine IRGs, AKT2, FGF7, FOS, IL27RA, LRP1, OBP2A, PAEP, PDGFRA, and PI3, that form a prognostic model in OC progression, distinguishing patients with significantly better clinical outcomes in the low-risk group. We validated this model as an independent prognostic indicator and demonstrated enhanced prognostic significance when used alongside clinical nomograms for accurate prediction. Elevated LRP1 expression, which indicates poor prognosis in bladder cancer (BLCA), OC, low-grade gliomas (LGG), and glioblastoma (GBM), was also associated with immune infiltration in several other cancers. Significant correlations with immune checkpoint genes (ICGs) highlight the potential importance of LRP1 as a biomarker and therapeutic target. Furthermore, gene set enrichment analysis highlighted LRP1’s involvement in metabolism-related pathways, supporting its prognostic and therapeutic relevance also in BLCA, OC, low-grade gliomas (LGG), GBM, kidney cancer, OC, BLCA, kidney renal clear cell carcinoma (KIRC), stomach adenocarcinoma (STAD), and stomach and oesophageal carcinoma (STES). Our study has generated a novel signature of nine IRGs within the TME across cancers, that could serve as potential prognostic predictors and provide a valuable resource to improve the prognosis of OC

Evaluation of Potential Antioxidant Activity of Leaves of Bauhinia Acuminate: Evaluation of Potential Antioxidant Activity of Leaves

The methanol extracts of leaves of Bauhinia acuminata (MESF) and their different fractions obtained from modified Kupchan partitioning method i.e. methanol, pet ether, carbon tetrachloride, chloroform, and aqueous soluble fractions were subjected to biological screening such as total phenolic content and antioxidant activity screening.The amount of total phenolic content differed in different extractives and ranged from 15.90 mg of GAE/gm of extractives to 124.80 mg of GAE /gm of extractives of B. acuminate. Among all extractives of B. acuminate the highest phenolic content was found in AQSF (124.80 mg of GAE /gm of extractives) followed by CSF (103.78 mg of GAE /gm of extractives).Significant amount of phenolic compounds also present in CTCSF (75.59 mg of GAE /gm of extractives), MESF (66.20 mg of GAE /gm of extractives) and PETSF (15.90 mg of GAE /gm of extractives) were also found. The antioxidant activity of IC50 values in DPPH method differed in different extractives and ranged from 22.01 to 77.79. Among all extractives of B. acuminata the highest free radical scavenging activity was given by CTCSF (absorbance 22.01) followed by AQSF (absorbance 29.83).Significant free radical scavenging activity was also exhibited by CSF (absorbance 45.09), MESF (absorbance 43.78), PESF (absorbance 77.79)

Present Prospectus of Malnutrition in Bangladesh: A Comprehensive Review

Abstract. Malnutrition is both over nutrition and under nutrition. As in Bangladesh the rapidity of under nutrition is too elevated, although slow, the rate of overweight and obesity in children and women is also rising. Under-nutrition results from micronutrient including essential vitamins and minerals deficiencies and both macro (protein energy deficiency). Malnutrition is anticipated to be causes of about 60% of maternal childhood and deaths in Bangladesh. Development in overall nutritional condition has been sluggish over the years. Rate of feasting and underweight both remain inappropriately very high in the country still

Susceptibility to patterns of ciprofloxacin among nalidixic acid-resistant Salmonella isolates collected in Banepa, Nepal from enteric fever patients

The present study determined the susceptibility to ciprofloxacin of nalidixic acid resistant Salmonella (NARS) isolated from enteric fever patients at Scheer Memorial Hospital, Banepa, Nepal, from June 2012 to December 2012. The antimicrobial sensitivity to nalidixic acid and ciprofloxacin was determined using modified Kirby-Bauer disc diffusion and broth dilution method according to the guidelines of the Clinical and Laboratory Standard Institute. Salmonella was isolated from 34 out of 992 (3.43%) blood cultures collected during the study period, and 10 (29.4%) isolates were identified as Salmonella enterica serotype Typhi, while 24 (70.6%) were identified as Salmonella enterica serotype Paratyphi. Out of the total isolates, 31 (91.2%) were nalidixic acid-resistant Salmonella (NARS). Among NARS, the minimum inhibitory concentration values for ciprofloxacin ranged from 0.25 to 2 mg/L and were constantly higher than those shown by the nalidixic acid-susceptible Salmonella. Therefore, in typhoid Salmonella nalidixic acid resistance may be the indicator of decreased susceptibility to ciprofloxacin

Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissueResearch in context

Background: The pharmacological activation of thermogenesis in brown adipose tissue has long been considered promising strategies to treat obesity. However, identification of safe and effective agents remains a challenge. In this study, we addressed this challenge by developing a cellular system with a fluorescence readout, and applied in a high-throughput manner to screen for FDA-approved drugs that may activate endogenous UCP1 expression in adipocytes. Methods: We have generated a Ucp1-2A-GFP reporter mouse, in which GFP intensity serves as a surrogate of the endogenous expression level of UCP1 protein; and immortalized brown adipocytes were derived from this mouse model and applied in drug screening. Candidate drugs were further tested in mouse models either fed with normal chow or high fat diet to induce obesity. Findings: By using the cellular screening platform, we identified a group of FDA-approved drugs that can upregulate UCP1 expression in brown adipocyte, including previously known UCP1 activators and new candidate drugs. Further studies focusing on a previously unreported drug—sutent, revealed that sutent treatment could increase the energy expenditure and inhibit lipid synthesis in mouse adipose and liver tissues, resulting in improved metabolism and resistance to obesity. Interpretation: This study offered an easy-to-use cellular screening system for UCP1 activators, and provided a candidate list of FDA-approved drugs that can potentially treat obesity. Further study of these candidates may shed new light on the drug discovery towards obesity. Fund: National Key Research and Development Program and the Strategic Priority Research Program of the Chinese Academy of Sciences, etc. (250 words). Keywords: Uncoupling protein 1, Brown adipose, FDA-approved drug library, Obesity, Suten