Potential of the essential oil from Pimenta pseudocaryophyllus as antimicrobial agent

This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6 %). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1.000 μg mL–1. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products

Tumor necrosis factor α, and agonist and antagonists of cannabinoid receptor type 1 and type 2 alter the immunophenotype of stem cells from human exfoliated deciduous teeth

ABSTRACT Objective To verify the involvement of the endocannabinoid system in the immunomodulatory profile of stem cells from human exfoliated deciduous teeth, in the presence or absence of TNF-α, and agonist and antagonists of CB1 and CB2. Methods Stem cells from human exfoliated deciduous teeth were cultured in the presence or absence of an agonist, anandamide, and two antagonists, AM251 and SR144528, of CB1 and CB2 receptors, with or without TNF-α stimulation. For analysis of immunomodulation, surface molecules linked to immunomodulation, namely human leukocyte antigen-DR isotype (HLA-DR), and programmed death ligands 1 (PD-L1) and 2 (PD-L2) were measured using flow cytometry. Results The inhibition of endocannabinoid receptors together with the proinflammatory effect of TNF-α resulted in increased HLA-DR expression in stem cells from human exfoliated deciduous teeth, as well as, in these cells acquiring an anti-inflammatory profile by enhancing the expression of PD-L1 and PD-L2. Conclusion Stem cells from human exfoliated deciduous teeth respond to the endocannabinoid system and TNF-α by altering key immune response molecules

Azastilbene Analogs as Tyrosinase Inhibitors: New Molecules with Depigmenting Potential

This research has been an effort to develop synthetic resveratrol analogs in order to improve the depigmenting potential of natural resveratrol. Six resveratrol analogs were synthesized and tested for tyrosinase inhibitory activity in vitro, by qualitative and quantitative steps. The results showed the analog C as being the most powerful tyrosinase inhibitor (IA50 = 65.67 ± 0.60 μg/mL), followed by the analogs B, E, F, A, and D, respectively. The analog C presented a tyrosinase inhibition potential better than natural resveratrol (P<0.001). The best depigmenting activity was provided by the presence of hydroxyl in the orthoposition on the second phenolic ring

Potential of the Essential Oil from Pimenta Pseudocaryophyllus as an Antimicrobial Agent

This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6 %). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1,000 μg mL-1. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products

The fracture evaluation of NiTi SMA endodontics files

The purpose of this study was to evaluate the fracture resistance and the fracture surface of NiTi SMA engine-driven endodontics files submitted to clockwise torsion. The maximum angular deflection and the maximum torque were analyzed without axial loading. The helical plastic deformations and the fracture surface morphology were evaluated by Scanning Electron Microscopic. The results showed that there was a significant statistical difference in the maximum fracture torque and no statistical difference for the angular deflection for the analyzed files. In relation to the maximum torque at the instant of the fracture, one of the brand presented better performance than the other. According to Scanning Electron Microscopic evaluation, all the files showed ductile fracture morphology

Isolamento de células-tronco de dentes decíduos exfoliados humanos e sua capacidade para diferenciação osteogênica

As células-tronco de dentes decíduos exfoliados, isoladas como uma subpopulação das células-tronco da polpa dentária, demonstram possuir a capacidade de se diferenciarem in vitro em neurônios, adipócitos, odontoblastos e osteoblastos. Devido a sua facilidade de obtenção, que não envolve um procedimento invasivo, esta linhagem celular é considerada uma grande promessa para a medicina regenerativa. Os objetivos deste trabalho foram estabelecer e caracterizar duas linhagens de células-tronco de dentes decíduos humanos. As linhagens foram isoladas a partir de amostras de crianças saudáveis de 6 anos de idade. Os resultados mostraram, por citometria de fluxo, que as células foram positivas para marcadores de células-tronco mesenquimais (CD105 (SH2), SH3 e SH4) e negativa para a marcação hematopoiética (CD45) e que as linhagens estabelecidaseram constituídas de uma população homogênea de células-tronco. As células obtidas foram avaliadas quanto a sua proliferação e capacidade de diferenciação osteogênica. As linhagens tiveram estatisticamente a mesma taxa de proliferação e foram capazes de diferenciação osteogênica

Satellite Cells: Regenerative Mechanisms and Applicability in Muscular Dystrophy

The satellite cells are long regarded as heterogeneous cell population, which is intimately linked to the processes of muscular recovery. The heterogeneous cell population may be classified by specific markers. In spite of the significant amount of variation amongst the satellite cell populations, it seems that their activity is tightly bound to the paired box 7 transcription factor expression, which is, therefore, used as a canonical marker for these cells. Muscular dystrophic diseases, such as Duchenne muscular dystrophy, elicit severe tissue injuries leading those patients to display a very specific pattern of muscular recovery abnormalities. There have been works on the application of precursors cells as a therapeutic alternative for Duchenne muscular dystrophy and initial attempts have proven the cells inefficient; however later endeavours have proposed solutions for the experiments improving significantly the results. The presence of a range of satellite cells populations indicates the existence of specific cells with enhanced capability of muscular recovery in afflicted muscles

Intestinal Microbiota as Modulators of the Immune System and Neuroimmune System: Impact on the Host Health and Homeostasis

Many immune-based intestinal disorders, such as ulcerative colitis and Crohn’s disease, as well as other illnesses, may have the intestines as an initial cause or aggravator in the development of diseases, even apparently not correlating directly to the intestine. Diabetes, obesity, multiple sclerosis, depression, and anxiety are examples of other illnesses discussed in the literature. In parallel, importance of the gut microbiota in intestinal homeostasis and immunologic conflict between tolerance towards commensal microorganisms and combat of pathogens is well known. Recent researches show that the immune system, when altered by the gut microbiota, influences the state in which these diseases are presented in the patient directly and indirectly. At the present moment, a considerable number of investigations about this subject have been performed and published. However, due to difficulties on correlating information, several speculations and hypotheses are generated. Thus, the present review aims at bringing together how these interactions work—gut microbiota, immune system, and their influence in the neuroimmune system