Isolation of ckit-positive cardiosphere-forming cells from human atrial biopsy

There is increasing interest in developing cell-based therapies to regenerate functional muscle and blood vessels in infarcted dysfunctional myocardium, using stem cells resident in the adult heart. The objective of our study was to identify an easy and cost-effective method for the isolation and expansion of human adult cardiac-resident stem cells. The cells were isolated from right atrial biopsy samples obtained from patients with ischemic heart disease, who were undergoing coronary artery bypass grafting. Two different isolation methods, enzymatic and nonenzymatic, were employed. The cell yield and cluster formation were not significantly different with either of the techniques used for cell isolation. The nonenzymatic method is recommended because of its simplicity and lower cost compared to the enzymatic method

Impact of electron beam and ethyl methane sulphonate on chlorophyll mutations in rice genotypes ASD 16 and Norungan

Induced mutagenesis facilitates the creation of novel gene combinations within a plant genome, preserving its basic structure. This study investigates the impact of electron beam radiation and ethyl methane sulphonate (EMS) on chlorophyll mutations in rice genotypes, ASD 16 and Norungan. The seeds were irradiated with five different doses of electron beam and EMS during rabi 2021-22. The M1 generation was assessed for seedling survival, seedling height and spikelet fertility followed by an identification of chlorophyll mutants in the M2 generation. At specific mutagen doses, ASD 16 and Norungan exhibited the genotypic difference for chlorophyll mutants. Various chlorophyll mutations, such as albino, chlorina, xantha, striata, viridis, albomaculata, alboviridis and xanthoviridis were observed. ASD 16 was more sensitive to both mutagens, while Norungan showed a broader response. EMS proved to be a more effective in inducing mutations than the electron beam. Lower and moderate mutagen doses demonstrated higher efficiency indicating the importance of optimizing mutagenic conditions. This study illuminates the significance of chlorophyll mutants genetic makeup varietal differences. The strong and diverse response observed in Norungan underscores its suitability for mutation breeding programmes. These findings contribute to the efficient utilisation of mutagenesis in improving rice traits providing practical implications for elevating crop quality and promoting genetic diversity in rice cultivation

Dynamical behavior of coumarin compounds in alcohol solvents

It is observed that, the rotational motion of probe molecule in methanol solvent increases as the temperature increase. We also investigated that, C30 rotates faster than C7 in the same solvent. It is all due to the friction between solute and solvent, diffusion and hydrogen bonding establish between alcoholic group and coumarin molecule. We have also determined the excited state and ground state dipole moments in alcoholic series solvents by using Bakhshiev, Chamma-viallet equations and Quantum mechanical treatment. From the results, it is found that, excited state dipole moment is greater than the ground state dipole moment, which indicates that excited state is more polar than the ground state

Sulfur Nanoparticles Synthesis and Characterization from H2S Gas, Using Novel Biodegradable Iron Chelates in W/O Microemulsion

Sulfur nanoparticles were synthesized from hazardous H2S gas using novel biodegradable iron chelates in w/o microemulsion system. Fe3+–malic acid chelate (0.05 M aqueous solution) was studied in w/o microemulsion containing cyclohexane, Triton X-100 andn-hexanol as oil phase, surfactant, co-surfactant, respectively, for catalytic oxidation of H2S gas at ambient conditions of temperature, pressure, and neutral pH. The structural features of sulfur nanoparticles have been characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), energy dispersive spectroscopy (EDS), diffused reflectance infra-red Fourier transform technique, and BET surface area measurements. XRD analysis indicates the presence of α-sulfur. TEM analysis shows that the morphology of sulfur nanoparticles synthesized in w/o microemulsion system is nearly uniform in size (average particle size 10 nm) and narrow particle size distribution (in range of 5–15 nm) as compared to that in aqueous surfactant systems. The EDS analysis indicated high purity of sulfur (>99%). Moreover, sulfur nanoparticles synthesized in w/o microemulsion system exhibit higher antimicrobial activity (against bacteria, yeast, and fungi) than that of colloidal sulfur

Polymorphic Cis- and Trans-Regulation of Human Gene Expression

Using genetic and molecular analyses, we identified over 1,000 polymorphic regulators that regulate expression levels of human genes

Effects of antiplatelet therapy on stroke risk by brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases: subgroup analyses of the RESTART randomised, open-label trial

Background Findings from the RESTART trial suggest that starting antiplatelet therapy might reduce the risk of recurrent symptomatic intracerebral haemorrhage compared with avoiding antiplatelet therapy. Brain imaging features of intracerebral haemorrhage and cerebral small vessel diseases (such as cerebral microbleeds) are associated with greater risks of recurrent intracerebral haemorrhage. We did subgroup analyses of the RESTART trial to explore whether these brain imaging features modify the effects of antiplatelet therapy

Analysis of Antibody and Cytokine Markers for Leprosy Nerve Damage and Reactions in the INFIR Cohort in India

Leprosy is one of the oldest known diseases. In spite of the established fact that it is least infectious and a completely curable disease, the social stigma associated with it still lingers in many countries and remains a major obstacle to self reporting and early treatment. The nerve damage that occurs in leprosy is the most serious aspect of this disease as nerve damage leads to progressive impairment and disability. It is important to identify markers of nerve damage so that preventive measures can be taken. This prospective cohort study was designed to look at the potential association of some serological markers with reactions and nerve function impairment. Three hundred and three newly diagnosed patients from north India were recruited for this study. The study attempts to reflect a model of nerve damage initiated by mycobacterial antigens and maintained by ongoing inflammation through cytokines such as Tumour Necrosis Factor alpha and perhaps extended by antibodies against nerve components