Innovating routes for the reused of PP-flax and PP-glass non woven composites: A comparative study

The significant industrial development of non-woven biocomposites requires the implementation of environmentally and economically coherent end-of-life recycling solutions. In this study, we studied the recycling of a non-woven poly-(propylene)-flax composite by injection but also by thermo compression. For comparison, a material with the same architecture but reinforced by glass fibres was studied. Both recycling methods showed strong specificities. Injection recycling leads to efficiently homogenised microstructures of the parts but also to drastically reduced lengths of the fibres, up to 10 times lower than with compression moulding. This method globally promotes high failure strengths while compression moulding, by preserving the length of the fibrous reinforcements, guarantees higher stiffness. This work also highlights the impacts of the length and division of the fibre elements on the microstructure of the injected parts; thus, after a series of compression recycling cycles, injected parts exhibit an important skin-core effect larger than after initial injection recycling cycles, whether in terms of orientation or local fibre volume fraction. As a consequence, after a series of recycling by compression, a new injection cycle has for effect to improve the tensile mechanical performances. For example, the strength and modulus of PP-flax composites are increased by 103% and 75%, respectively. These results highlight the technical feasibility and relevance of implementing these two recycling methods, depending on the volumes or equipment available and the final properties to promote, as they enable the production of new highperformance parts

Exploring two innovative recycling ways for poly-(propylene)-flax non wovens wastes

Biocomposites have major advantage in term of weight saving thanks to lower densities compared to conventional materials; this property also allows a reduction of environmental impacts which is beneficial for the automotive industry. In addition, they maintain rather good mechanical properties after recycling cycles which makes possible to consider other end-of-life scenarios than incineration or landfill. In this work, we studied two potential routes for the recycling of moulding wastes from poly (propylene)-flax nonwovens. By representing close to 25%-wt, these wastes are a major industrial problem, inducing additional recycling or treatment costs. In a first step, the parts were grinded and reincorporated at different weight fractions into virgin nonwovens. Up to 30%-wt of reincorporated wastes, results showed good mechanical performances for recycled nonwovens, especially in bending mode. In a second stage, scraps were grinded and then compounded for injection moulding applications. Their good rheological behavior and ability to conserve fibre lengths during extrusion made possible to formulate competitive materials in terms of tensile performances. (C) 2017 Elsevier Ltd. All rights reserved

Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design

Abstract The promises of vaccines based on virus-like particles stimulate demand for universal non-infectious virus-like platforms that can be efficiently grafted with large antigens. Here we harnessed the modularity and extreme affinity of the decoration protein pb10 for the capsid of bacteriophage T5. SPR experiments demonstrated that pb10 fused to mCherry or to the model antigen ovalbumin (Ova) retained picomolar affinity for DNA-free T5 capsid-like particles (T5-CLPs), while cryo-EM studies attested to the full occupancy of the 120 capsid binding sites. Mice immunisation with CLP-bound pb10-Ova chimeras elicited strong long-lasting anti-Ova humoral responses involving a large panel of isotypes, as well as CD8 + T cell responses, without any extrinsic adjuvant. Therefore, T5-CLP constitutes the first DNA-free bacteriophage capsid able to irreversibly display a regular array of large antigens through highly efficient chemical-free anchoring. Its ability to elicit robust immune responses paves the way for further development of this novel vaccination platform

Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design

Posted November 04, 2022 on bioRxiv.The promises of vaccines based on virus-like particles stimulate demand for universal non-infectious virus-like platforms that can be efficiently grafted with large antigens. Here we harnessed the modularity and extreme affinity of the decoration protein pb10 for the capsid of bacteriophage T5. SPR experiments demonstrated that pb10 fused to mCherry or to the model antigen ovalbumin (Ova) retained picomolar affinity for DNA-free T5 capsid-like particles (T5-CLPs), while cryo-EM studies attested to the full occupancy of the 120 capsid binding sites. Mice immunisation with CLP-bound pb10-Ova chimeras elicited strong long-lasting anti-Ova humoral responses involving a large panel of isotypes, as well as CD8 + T cell responses, without any extrinsic adjuvant. Therefore, T5-CLP constitutes the first DNA-free bacteriophage capsid able to irreversibly display a regular array of large antigens through highly efficient chemical-free anchoring. Its ability to elicit robust immune responses paves the way for further development of this novel vaccination platform

Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design

Posted November 04, 2022 on bioRxiv.The promises of vaccines based on virus-like particles stimulate demand for universal non-infectious virus-like platforms that can be efficiently grafted with large antigens. Here we harnessed the modularity and extreme affinity of the decoration protein pb10 for the capsid of bacteriophage T5. SPR experiments demonstrated that pb10 fused to mCherry or to the model antigen ovalbumin (Ova) retained picomolar affinity for DNA-free T5 capsid-like particles (T5-CLPs), while cryo-EM studies attested to the full occupancy of the 120 capsid binding sites. Mice immunisation with CLP-bound pb10-Ova chimeras elicited strong long-lasting anti-Ova humoral responses involving a large panel of isotypes, as well as CD8 + T cell responses, without any extrinsic adjuvant. Therefore, T5-CLP constitutes the first DNA-free bacteriophage capsid able to irreversibly display a regular array of large antigens through highly efficient chemical-free anchoring. Its ability to elicit robust immune responses paves the way for further development of this novel vaccination platform

Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design

International audienceThe promises of vaccines based on virus-like particles stimulate demand for universal non-infectious virus-like platforms that can be efficiently grafted with large antigens. Here, we harnessed the modularity and extreme affinity of the decoration protein pb10 for the capsid of bacteriophage T5. SPR experiments demonstrated that pb10 fused to mCherry or to the model antigen ovalbumin (Ova) retained picomolar affinity for DNA-free T5 capsid-like particles (T5-CLPs), while cryo-EM studies attested to the full occupancy of the 120 capsid binding sites. Mice immunization with CLP-bound pb10-Ova chimeras elicited strong long-lasting anti-Ova humoral responses involving a large panel of isotypes, as well as CD8 + T cell responses, without any extrinsic adjuvant. Therefore, T5-CLP constitutes a unique DNA-free bacteriophage capsid able to display a regular array of large antigens through highly efficient chemical-free anchoring. Its ability to elicit robust immune responses paves the way for further development of this novel vaccination platform