Mapping the energy and diffusion landscapes of membrane proteins at the cell surface using high-density single-molecule imaging and Bayesian inference: application to the multi-scale dynamics of glycine receptors in the neuronal membrane

Protein mobility is conventionally analyzed in terms of an effective diffusion. Yet, this description often fails to properly distinguish and evaluate the physical parameters (such as the membrane friction) and the biochemical interactions governing the motion. Here, we present a method combining high-density single-molecule imaging and statistical inference to separately map the diffusion and energy landscapes of membrane proteins across the cell surface at ~100 nm resolution (with acquisition of a few minutes). When applying these analytical tools to glycine neurotransmitter receptors (GlyRs) at inhibitory synapses, we find that gephyrin scaffolds act as shallow energy traps (~3 kBT) for GlyRs, with a depth modulated by the biochemical properties of the receptor-gephyrin interaction loop. In turn, the inferred maps can be used to simulate the dynamics of proteins in the membrane, from the level of individual receptors to that of the population, and thereby, to model the stochastic fluctuations of physiological parameters (such as the number of receptors at synapses). Overall, our approach provides a powerful and comprehensive framework with which to analyze biochemical interactions in living cells and to decipher the multi-scale dynamics of biomolecules in complex cellular environments.Comment: 23 pages, 4 figure

WET SCRUBBING INTENSIFICATION APPLIED TO HYDROGEN SULPHIDE REMOVAL IN WASTE WATER TREATMENT PLANT

International audienceHydrogen sulphide removal in a Waste Water Treatment Plant at semi-industrial scale in a compact wet scrubber has been investigated. The gas residence time in the scrubber was reduced to 30 ms using a NaOCl caustic scrubbing solution. The contactor is composed of a wire mesh packing structure where liquid and gas flow co-currently at high velocity (> 12 m.s-1). H2S removal percentages higher than 95% could be achieved whereas a moderate pressure drop was measured (< 4000 Pa). Both the hydrodynamic and chemical conditions can influence the efficiency of the process. Correlations were developed to predict both the pressure drop and the H2S removal efficiency at given operating conditions

Traitement de composés organiques volatils par lavage chimique compact: recyclage de la solution de lavage par oxydation avancée O3/H2O2

National audienceThis study focuses on Volatile Organic Compounds (VOC) treatment in a compact chemical scrubber composed of a structured packing operating at co-current and high gas superficial velocity (> 10 m.s-1). The scrubbing liquid is composed of ozone and hydrogen peroxide to generate very reactive hydroxyl radicals. Results demonstrate a high mineralisation rate of the transferred VOC, even with low oxidant concentrations. Therefore, the scrubbing liquid can be recycled and recirculated at the top of the scrubber without efficiency drop over time. Neutral VOC removal efficiency increases with their solubility in water, from 14% for dimethyldisulfide to 86% for butanol using a mass flow rates ratio (L/G) of 2.5 and residence time of 20 ms. For trimethylamine, a basic VOC, the increased apparent solubility at pH = 8 leads to a high removal efficiency of 80%. Major by-products were identified and quantified. An absence of stripping in the treated gas emphasizes their innocuity.Cette étude porte sur le traitement de Composés Organiques Volatils (COV) dans un laveur chimique compact composé d’un garnissage structuré fonctionnant à co-courant et à vitesse superficielle du gaz élevée (> 10 m.s-1). La solution aqueuse de lavage se compose d’un mélange d’ozone et de peroxyde d’hydrogène afin de générer des radicaux hydroxyles très réactifs. Les résultats démontrent une minéralisation poussée des COV transférés même avec des concentrations en oxydants modestes. La solution de lavage peut donc être régénérée et réinjectée en tête du laveur sans diminution d’efficacité au cours du temps. L’abattement en phase gazeuse des COV neutres, observés pour un rapport des débits massiques L/G de 2,5, augmente avec leur solubilité en phase aqueuse, démarrant à 14% pour le dimethyldisulfide jusqu’à 86% pour le butanol en 20 ms de temps de contact. Pour la triméthylamine, composé basique, l’augmentation de la solubilité apparente à pH 8 permet d’atteindre un abattement élevé de 80%. Les sous-produits majoritaires ont été identifiés et quantifiés. L’absence de stripping dans le gaz traité met en évidence leur innocuité

Hydrogen sulphide removal in waste water treatment plant by compact oxidative scrubbing in Aquilair PlusTM process

International audienceRecently, the development of a high voidage contactor, named Aquilair Plus™, has demonstrated high efficiency for chemical scrubbing of hydrogen sulphide. Liquid and gas phases flow at co-current and high velocity, leading to a great dispersion of the liquid and then to an enhancement of the mass transfer rate by comparison with classical packed towers. This study focused on the results which obtained at semi-industrial scale with the Aquilair PlusTM process on a waste water treatment plant located in France. The scrubbing liquid consisted of a sodium hypochlorite alkaline solution. At once, pressure drop, H2S removal and reagents consumption were followed. The influence of the superficial gas velocity, liquid-to-gas mass ratio (L/G), pH, hypochlorite concentration of the scrubbing liquid and H2S inlet concentration was characterised. H2S removal percentages higher than 90% could be easily achieved with a moderate pressure drop (< 40 mbar). Both hydrodynamic and chemical conditions proved to influence performances. Reagents consumptions slightly higher than the predicted ones were measured

Prospectus, November 8, 1978

SHOPLIFTING--A GROWING CRIME; Recital cancelled; Activities for the week; Registration still open for bridge tournament; Register by tomorrow for adult workshop; PC offers glass class; Everyone feels depression; Counseling center hosts transfer day; Shopping trip set for Dec. 2; PC players begin play; \u27Super Piano\u27 performs at PC; Shoplifting problem turns to part-time robbery; Bread warms audience in Halloween concert; Queen contest set for Dec. 15; NJCAA meet hosted by PC; PC students put their best pumpkin forward; Classifieds; DePree gives Karate exhibit; PC offers workshop; WPCD\u27s Top 15 for the Week of Nov. 6; State Tourney here we come!; Intramurals start this week; Illini team is promising; Fast Freddy Contest; Clarke wins Fast Freddy; PC finishes 3rd at Danvillehttps://spark.parkland.edu/prospectus_1978/1004/thumbnail.jp

Prospectus, October 18, 1978

PARKLAND\u27S BOOKSTORE-- IS IT RIPPING YOU OFF?; letters to the editor One student does not want gay bars to turn into \u27freak shows\u27; Give blood today; College Cuisine; Dec. 31 is EC fellowship application deadline; Advertising Policy; Instructors attend meeting next week; Electronics \u27bugs\u27 invited to Parkland; Gammon elected charter member; Riding club has contest; Raffle winners have last day to claim prizes; Language clubs will have dinner; Nov. 5 poetry review deadline; PC defines typical student; Taiwan students to give performance at U of I; SNAP to host guest speakers; Parkland is getting ready for hard winter; PC math contest to be Saturday, April 28; Mimes visit campus; Legal clinic is Oct. 19; Farm emergency workshop to be held on Nov. 4; Women\u27s creativity honored; Parkland\u27s nursing program is Gogol\u27s first full-time job; High cost of books is common problem; DeShong attacking PC apathy; PC health service sponsors lectures; WPCD\u27s Top 15 For The Week Of Oct. 9; Final Christmas bazaar meeting; \u27I Quit Smoking\u27 draws fifteen; PC events for the week; Battered women series part 2: Battered women face struggles; Craft media in art exhibit; Free Classifieds; Informants can profit; Family Service conducts workshop; Golf team places 20th; PC still looking for wrestlers; Paxton still undefeated; \u27Spikers\u27 ready for tourney; Intramural basketball team organizing now; B-ball meeting for girls to be tuesday; Fast Freddy Contest; Fast Freddy has a winner with three wrong answershttps://spark.parkland.edu/prospectus_1978/1007/thumbnail.jp

Improved DIQKD protocols with finite-size analysis

The security of finite-length keys is essential for the implementation of device-independent quantum key distribution (DIQKD). Presently, there are several finite-size DIQKD security proofs, but they are mostly focused on standard DIQKD protocols and do not directly apply to the recent improved DIQKD protocols based on noisy preprocessing, random key measurements, and modified CHSH inequalities. Here, we provide a general finite-size security proof that can simultaneously encompass these approaches, using tighter finite-size bounds than previous analyses. In doing so, we develop a method to compute tight lower bounds on the asymptotic keyrate for any such DIQKD protocol with binary inputs and outputs. With this, we show that positive asymptotic keyrates are achievable up to depolarizing noise values of $9.33\%$, exceeding all previously known noise thresholds. We also develop a modification to random-key-measurement protocols, using a pre-shared seed followed by a "seed recovery" step, which yields substantially higher net key generation rates by essentially removing the sifting factor. Some of our results may also improve the keyrates of device-independent randomness expansion.Comment: Improved threshold with more data points, discussion of conjecture in [SGP+21], correction regarding results of [MDR+19