Genome Sequencing Reveals Widespread Virulence Gene Exchange among Human Neisseria Species

Commensal bacteria comprise a large part of the microbial world, playing important roles in human development, health and disease. However, little is known about the genomic content of commensals or how related they are to their pathogenic counterparts. The genus Neisseria, containing both commensal and pathogenic species, provides an excellent opportunity to study these issues. We undertook a comprehensive sequencing and analysis of human commensal and pathogenic Neisseria genomes. Commensals have an extensive repertoire of virulence alleles, a large fraction of which has been exchanged among Neisseria species. Commensals also have the genetic capacity to donate DNA to, and take up DNA from, other Neisseria. Our findings strongly suggest that commensal Neisseria serve as reservoirs of virulence alleles, and that they engage extensively in genetic exchange

Enfermedad de células falciformes: Un diagnóstico para tener presente

Sickle cell disease (SCD) is an autosomal recessive genetic disorder. It is the most frequent structural hemoglobinopathy worldwide, and it is produced by an alteration in the globin chain genes. In Chile, there is no data on the prevalence of SCD since it is considered a very rare condition. The incidence of this disease has been increasing due to migration of people from areas with greater presence of SCD. It is important to know and consider this diagnosis in a selected group of patients with anemia, in order to prevent and treat the different complications of this disease. This article reviews the most recent information that shows new concepts in the knowledge of the physiopathology, and especially publications of guidelines and consensus in relation to the diagnosis and management of this condition. © 2018, Sociedad Chilena de Pediatria. All rights reserved

Enfermedad de células falciformes: Un diagnóstico para tener presente

Sickle cell disease (SCD) is an autosomal recessive genetic disorder. It is the most frequent structural hemoglobinopathy worldwide, and it is produced by an alteration in the globin chain genes. In Chile, there is no data on the prevalence of SCD since it is considered a very rare condition. The incidence of this disease has been increasing due to migration of people from areas with greater presence of SCD. It is important to know and consider this diagnosis in a selected group of patients with anemia, in order to prevent and treat the different complications of this disease. This article reviews the most recent information that shows new concepts in the knowledge of the physiopathology, and especially publications of guidelines and consensus in relation to the diagnosis and management of this condition. © 2018, Sociedad Chilena de Pediatria. All rights reserved

Search for heavy resonances and quantum black holes in eμ\mu, eτ\tau, and μτ\mu\tau final states in proton-proton collisions at s\sqrt{s} = 13 TeV

A search is reported for heavy resonances and quantum black holes decaying into e$\mu$, e$\tau$, and $\mu\tau$ final states in proton-proton collision data recorded by the CMS experiment at the CERN LHC during 2016-2018 at $\sqrt{s}$ = 13 TeV, corresponding to an integrated luminosity of 138 fb$^{-1}$. The e$\mu$, e$\tau$, and $\mu\tau$ invariant mass spectra are reconstructed, and no evidence is found for physics beyond the standard model. Upper limits are set at 95% confidence level on the product of the cross section and branching fraction for lepton flavor violating signals. Three benchmark signals are studied: resonant $\tau$ sneutrino production in $R$ parity violating supersymmetric models, heavy Z' gauge bosons with lepton flavor violating decays, and nonresonant quantum black hole production in models with extra spatial dimensions. Resonant $\tau$ sneutrinos are excluded for masses up to 4.2 TeV in the e$\mu$ channel, 3.7 TeV in the e$\tau$ channel, and 3.6 TeV in the $\mu\tau$ channel. A Z' boson with lepton flavor violating couplings is excluded up to a mass of 5.0 TeV in the e$\mu$ channel, up to 4.3 TeV in the e$\tau$ channel, and up to 4.1 TeV in the $\mu\tau$ channel. Quantum black holes in the benchmark model are excluded up to the threshold mass of 5.6 TeV in the e$\mu$ channel, 5.2 TeV in the e$\tau$ channel, and 5.0 TeV in the $\mu\tau$ channel. In addition, model-independent limits are extracted to allow comparisons with other models for the same final states and similar event selection requirements. The results of these searches provide the most stringent limits available from collider experiments for heavy particles that undergo lepton flavor violating decays

Search for direct pair production of supersymmetric partners of τ\tau leptons in the final state with two hadronically decaying τ\tau leptons and missing transverse momentum in proton-proton collisions at s\sqrt{s} = 13 TeV

A search for the direct production of a pair of $τ$ sleptons, the supersymmetric partners of $τ$ leptons, is presented. Each $τ$ slepton is assumed to decay to a $τ$ lepton and the lightest supersymmetric particle (LSP), which is assumed to be stable and to not interact in the detector, leading to an imbalance in the total reconstructed transverse momentum. The search is carried out in events identified as containing two $τ$ leptons, each decaying to one or more hadrons and a neutrino, and significant transverse momentum imbalance. In addition to scenarios in which the $τ$ sleptons decay promptly, the search also addresses scenarios in which the $τ$ sleptons have sufficiently long lifetimes to give rise to nonprompt $τ$ leptons. The data were collected in proton-proton collisions at a center-of-mass energy of 13 TeV at the CERN LHC with the CMS detector in 2016–2018, and correspond to an integrated luminosity of 138 fb$^{-1}$. No significant excess is seen with respect to standard model expectations. Upper limits on cross sections for the pair production of $τ$ sleptons are obtained in the framework of simplified models. In a scenario in which the $τ$ sleptons are superpartners of left-handed $τ$ leptons, and each undergoes a prompt decay to a $τ$ lepton and a nearly massless LSP, $τ$ slepton masses between 115 and 340 GeV are excluded. In a scenario in which the lifetime of the $τ$ sleptons corresponds to $cτ_0$ = 0.1 mm, where $τ_0$ represents the mean proper lifetime of the $τ$ slepton, masses between 150 and 220 GeV are excluded

Search for heavy resonances and quantum black holes in eμ\mu, eτ\tau, and μτ\mu\tau final states in proton-proton collisions at s\sqrt{s} = 13 TeV

A search is reported for heavy resonances and quantum black holes decaying into e$\mu$, e$\tau$, and $\mu\tau$ final states in proton-proton collision data recorded by the CMS experiment at the CERN LHC during 2016-2018 at $\sqrt{s} =$ 13 TeV, corresponding to an integrated luminosity of 138 fb$^{-1}$. The e$\mu$, e$\tau$, and $\mu\tau$ invariant mass spectra are reconstructed, and no evidence is found for physics beyond the standard model. Upper limits are set at 95% confidence level on the product of the cross section and branching fraction for lepton flavor violating signals. Three benchmark signals are studied: resonant $\tau$ sneutrino production in $R$ parity violating supersymmetric models, heavy Z' gauge bosons with lepton flavor violating decays, and nonresonant quantum black hole production in models with extra spatial dimensions. Resonant $\tau$ sneutrinos are excluded for masses up to 4.2 TeV in the e$\mu$ channel, 3.7 TeV in the e$\tau$ channel, and 3.6 TeV in the $\mu\tau$ channel. A Z' boson with lepton flavor violating couplings is excluded up to a mass of 5.0 TeV in the e$\mu$ channel, up to 4.3 TeV in the e$\tau$ channel, and up to 4.1 TeV in the $\mu\tau$ channel. Quantum black holes in the benchmark model are excluded up to the threshold mass of 5.6 TeV in the e$\mu$ channel, 5.2 TeV in the e$\tau$ channel, and 5.0 TeV in the $\mu\tau$ channel. In addition, model-independent limits are extracted to allow comparisons with other models for the same final states and similar event selection requirements. The results of these searches provide the most stringent limits available from collider experiments for heavy particles that undergo lepton flavor violating decays.A search is reported for heavy resonances and quantum black holes decaying into eμ, eτ, and μτ final states in proton-proton collision data recorded by the CMS experiment at the CERN LHC during 2016–2018 at $\sqrt{s}$ = 13 TeV, corresponding to an integrated luminosity of 138 fb$^{−1}$. The eμ, eτ, and μτ invariant mass spectra are reconstructed, and no evidence is found for physics beyond the standard model. Upper limits are set at 95% confidence level on the product of the cross section and branching fraction for lepton flavor violating signals. Three benchmark signals are studied: resonant τ sneutrino production in R parity violating supersymmetric models, heavy Z′ gauge bosons with lepton flavor violating decays, and nonresonant quantum black hole production in models with extra spatial dimensions. Resonant τ sneutrinos are excluded for masses up to 4.2TeV in the eμ channel, 3.7TeV in the eτ channel, and 3.6TeV in the μτ channel. A Z′ boson with lepton flavor violating couplings is excluded up to a mass of 5.0TeV in the eμ channel, up to 4.3Te V in the eτ channel, and up to 4.1TeV in the μτ channel. Quantum black holes in the benchmark model are excluded up to the threshold mass of 5.6TeV in the eμ channel, 5.2TeV in the eτ channel, and 5.0TeV in the μτ channel. In addition, model-independent limits are extracted to allow comparisons with other models for the same final states and similar event selection requirements. The results of these searches provide the most stringent limits available from collider experiments for heavy particles that undergo lepton flavor violating decays.[graphic not available: see fulltext]A search is reported for heavy resonances and quantum black holes decaying into e$\mu$, e$\tau$, and $\mu\tau$ final states in proton-proton collision data recorded by the CMS experiment at the CERN LHC during 2016-2018 at $\sqrt{s}$ = 13 TeV, corresponding to an integrated luminosity of 138 fb$^{-1}$. The e$\mu$, e$\tau$, and $\mu\tau$ invariant mass spectra are reconstructed, and no evidence is found for physics beyond the standard model. Upper limits are set at 95% confidence level on the product of the cross section and branching fraction for lepton flavor violating signals. Three benchmark signals are studied: resonant $\tau$ sneutrino production in $R$ parity violating supersymmetric models, heavy Z' gauge bosons with lepton flavor violating decays, and nonresonant quantum black hole production in models with extra spatial dimensions. Resonant $\tau$ sneutrinos are excluded for masses up to 4.2 TeV in the e$\mu$ channel, 3.7 TeV in the e$\tau$ channel, and 3.6 TeV in the $\mu\tau$ channel. A Z' boson with lepton flavor violating couplings is excluded up to a mass of 5.0 TeV in the e$\mu$ channel, up to 4.3 TeV in the e$\tau$ channel, and up to 4.1 TeV in the $\mu\tau$ channel. Quantum black holes in the benchmark model are excluded up to the threshold mass of 5.6 TeV in the e$\mu$ channel, 5.2 TeV in the e$\tau$ channel, and 5.0 TeV in the $\mu\tau$ channel. In addition, model-independent limits are extracted to allow comparisons with other models for the same final states and similar event selection requirements. The results of these searches provide the most stringent limits available from collider experiments for heavy particles that undergo lepton flavor violating decays

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)

Search for heavy resonances decaying to a pair of Lorentz-boosted Higgs bosons in final states with leptons and a bottom quark pair at s \sqrt{s} = 13 TeV

A search for new heavy resonances decaying to a pair of Higgs bosons (HH) in proton-proton collisions at a center-of-mass energy of 13 TeV is presented. Data were collected with the CMS detector at the LHC in 2016–2018, corresponding to an integrated luminosity of 138 fb$^{−1}$. Resonances with a mass between 0.8 and 4.5 TeV are considered using events in which one Higgs boson decays into a bottom quark pair and the other into final states with either one or two charged leptons. Specifically, the single-lepton decay channel $\mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}{\mathrm{WW}}^{\ast}\to \mathrm{b}\overline{\mathrm{b}}\ell v\mathrm{q}{\overline{\mathrm{q}}}^{\prime }$ and the dilepton decay channels $\mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}{\mathrm{WW}}^{\ast}\to \mathrm{b}\overline{\mathrm{b}}\ell v\ell v$ and $\mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}\tau \tau \to \mathrm{b}\overline{\mathrm{b}}\ell vv\ell vv$ are examined, where ℓ in the final state corresponds to an electron or muon. The signal is extracted using a two-dimensional maximum likelihood fit of the $\mathrm{H}\to \mathrm{b}\overline{\mathrm{b}}$ jet mass and HH invariant mass distributions. No significant excess above the standard model expectation is observed in data. Model-independent exclusion limits are placed on the product of the cross section and branching fraction for narrow spin-0 and spin-2 massive bosons decaying to HH. The results are also interpreted in the context of radion and bulk graviton production in models with a warped extra spatial dimension. The results provide the most stringent limits to date for X → HH signatures with final-state leptons and at some masses provide the most sensitive limits of all X → HH searches