Reciprocity between abscisic acid and ethylene at the onset of berry ripening and after harvest

<p>Abstract</p> <p>Background</p> <p>The ripening of grape berry is generally regulated by abscisic acid (ABA), and has no relationship with ethylene function. However, functional interaction and synergism between ABA and ethylene during the beginning of grape berry ripening (véraison) has been found recently.</p> <p>Results</p> <p>The expressions of <it>VvNCED1 </it>encoding 9-<it>cis</it>-epoxycarotenoid dioxygenase (NCED) and <it>VvGT </it>encoding ABA glucosyltransferase were all increased rapidly at the stage of véraison and reached the highest level at 9th week after full bloom. However, <it>VvCYP1 </it>encoding ABA 8'-hydroxylase and <it>VvβG1 </it>encoding berry β-glucosidase are different, whose expression peak appeared at the 10th week after full bloom and in especial <it>VvβG1 </it>remained at a high level till harvest. The <it>VvACO1 </it>encoding 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase, the <it>VvETR2 </it>(ethylene response 2) and <it>VvCTR1 </it>(constitutive triple response 1) had a transient expression peak at pre-véraison, while the <it>VvEIN4 </it>(ethylene insensitive 4) expression gradually increased from the véraison to one week before harvest stage. The above mentioned changes happened again in the berry after harvest. At one week before véraison, double block treatment with NiCl₂plus 1-methylcyclopropene (1-MCP) not only inhibited the release of ethylene and the expression of related genes but also suppressed the transcription of <it>VvNCED1 </it>and the synthesis of ABA which all might result in inhibiting the fruit ripening onset. Treatment with ABA could relieve the double block and restore fruit ripening course. However, after harvest, double block treatment with NiCl₂plus 1-MCP could not suppress the transcription of <it>VvNCED1 </it>and the accumulation of ABA, and also could not inhibit the start of fruit senescence.</p> <p>Conclusion</p> <p>The trace endogenous ethylene induces the transcription of <it>VvNCED1 </it>and then the generation of ABA followed. Both ethylene and ABA are likely to be important and their interplaying may be required to start the process of berry ripening. When the level of ABA reached the peak value, part of it will be stored in the form of ABA-GE. While after harvest, abiotic stresses principally (such as dehydration, harvest shock) could induce the transcription of <it>VvNCED1 </it>and the accumulation of ABA, thus starting the process of fruit senescence.</p

Hand palm local channel characterization for millimeter-wave body-centric applications

The body-centric wireless channel characterization mostly utilizes whole body models. However, localized channels for body parts consistently interacting with the wireless device have their own importance. This paper attempts to characterize the hand palm local channel through experimental measurements at three millimeter-wave frequency bands of 27-28 GHz, 29-30 GHz, and 31-32 GHz. Five human subjects are used in this study. Net body loss is found to be 3dB for different subjects with subject-specific and varying palm shape size is found to be the primary affecting source. The repeatability of the on-body propagation measurements is found to be within 10% of variance

Authentication in Millimeter-Wave Body-Centric Networks Through Wireless Channel Characterization

Advent of 5G technologies has ensued in massive growth of body-centric communications (BCCs), especially at millimeter-wave (mm-wave) frequencies. As a result, the portable/handheld terminals are becoming more and more “intelligent” but not without the cost of being less secure. Improved authentication measures need to be explored, as effective identity authentication is the first level of security in these devices. This paper presents a novel keyless authentication method exploiting wireless channel characteristics. Human palm has distinct transmission coefficient (S21) for each of the users and is used for in vivo fingerprint identification in this paper. A detailed channel modeling using data acquisition from real environment and empirical approach is adopted to evaluate the usability of this method. The results show that this method can provide a secure operation for the mm-wave 5G BCCs

Circulating MicroRNA Profiles Differ between Qi-Stagnation and Qi-Deficiency in Coronary Heart Disease Patients with Blood Stasis Syndrome

We compared the circulating microRNA profiles of Qi-stagnation (QSB) and Qi-deficiency (QDB) in coronary heart disease (CHD) patients with blood stasis syndrome. Twenty-nine CHD patients were divided into QSB group and QDB group. The analysis was carried out through comparing their circulating microRNA profiles and the following bioinformatics analysis. The number of differential miRNAs in QDB group was much more than that in QSB group. Functional annotations of the differentially expressed miRNAs target genes in the QSB group and QDB group were, respectively, related to regulation of cellular component organization, regulation of glucose metabolic process, and so forth and protein kinase cascade, phosphate metabolic process, and so forth. KEGG pathway analysis showed that the process Qi-deficiency was associated with phagocytosis including endocytosis and mTOR signaling pathway. Specifically, pathway of cell adhesion molecules played the crucial role in the pathological process of Qi-stagnation, with a unique upregulation except for pathways associated with cancer signal. MicroRNA-gene-net analysis indicated that let-7c, miR-4487, miR-619, miR-8075, miR-6735, and miR-32-5p and miR-17-5p, miR-130a, and miR 320 family had the most important and extensive regulatory function for Qi-stagnation syndromes and Qi-deficiency syndromes, respectively. Differentially expressed miRNAs and concerned pathways suggest different molecular mechanisms that may mediate the pathological process of QSB and QDB syndromes

Performing group-level functional image analyses based on homologous functional regions mapped in individuals

Functional MRI (fMRI) studies have traditionally relied on intersubject normalization based on global brain morphology, which cannot establish proper functional correspondence between subjects due to substantial intersubject variability in functional organization. Here, we reliably identified a set of discrete, homologous functional regions in individuals to improve intersubject alignment of fMRI data. These functional regions demonstrated marked intersubject variability in size, position, and connectivity. We found that previously reported intersubject variability in functional connectivity maps could be partially explained by variability in size and position of the functional regions. Importantly, individual differences in network topography are associated with individual differences in task-evoked activations, suggesting that these individually specified regions may serve as the localizer to improve the alignment of task-fMRI data. We demonstrated that aligning task-fMRI data using the regions derived from resting state fMRI may lead to increased statistical power of task-fMRI analyses. In addition, resting state functional connectivity among these homologous regions is able to capture the idiosyncrasies of subjects and better predict fluid intelligence (gF) than connectivity measures derived from group-level brain atlases. Critically, we showed that not only the connectivity but also the size and position of functional regions are related to human behavior. Collectively, these findings suggest that identifying homologous functional regions across individuals can benefit a wide range of studies in the investigation of connectivity, task activation, and brain-behavior associations. Author summary No two individuals are alike. The size, shape, position, and connectivity patterns of brain functional regions can vary drastically between individuals. While interindividual differences in functional organization are well recognized, to date, standard procedures for functional neuroimaging research still rely on aligning different subjects' data to a nominal average brain based on global brain morphology. We developed an approach to reliably identify homologous functional regions in each individual and demonstrated that aligning data based on these homologous functional regions can significantly improve the study of resting state functional connectivity, task-fMRI activations, and brain-behavior associations. Moreover, we showed that individual differences in size, position, and connectivity of brain functional regions are dissociable, and each can provide nonredundant information in explaining human behavior

Shuangshen Ningxin Capsule, a Traditional Chinese Medicinal Preparation, Alleviates Myocardial Ischemia through Autophagy Regulation

Shuangshen Ningxin capsule (SSNX), a modern Chinese formula, has been used to treat cardiovascular diseases in Eastern Asia. Our study focuses on the autophagy regulation of SSNX against coronary artery injuries. Myocardial infarction model was established in Chinese miniswines (CMS) by coronary artery balloon injury. SSNX was administered to the CMS for 8 weeks with 4 mg/kg or 16 mg/kg. Myocardial cells were incubated with 20% SSNX medicated serum for 2 hours. Assays were performed to detect the effects of SSNX on (i) coronary artery diameter by angiography, (ii) hemodynamics by noninvasive hemodynamic monitoring system, (iii) plaque burden and plaque volume by intravenous ultrasound (iv) coronary artery histology by H&E staining, (v) autophagosome by transmission electron microscopy, (vi) lactate dehydrogenase (LDH) leakage, and (vii) Beclin-1 and LC3-I/II expressions by Western blot. The results showed that CMS treated with SSNX exhibited the correction for the disturbed cardiac hemodynamics, increase of coronary artery diameter, reduction of high plaque burden and plaque volume, and decrease of LDH. The inhibitory effect of SSNX on CMS autophagy was demonstrated by the reduction of autophagosome and the downregulation of beclin-1 and LC3-I/II. SSNX may protect coronary artery and increase the stability of plaque through the suppression of myocardial cellular autophagy, which suggests the potentially therapeutic effect of SSNX on ischemic cardiovascular disease

Noninvasive suspicious liquid detection using wireless signals

Conventional liquid detection instruments are very expensive and not conducive to large-scale deployment. In this work, we propose a method for detecting and identifying suspicious liquids based on the dielectric constant by utilizing the radio signals at a 5G frequency band. There are three major experiments: first, we use wireless channel information (WCI) to distinguish between suspicious and nonsuspicious liquids; then we identify the type of suspicious liquids; and finally, we distinguish the different concentrations of alcohol. The K-Nearest Neighbor (KNN) algorithm is used to classify the amplitude information extracted from the WCI matrix to detect and identify liquids, which is suitable for multimodal problems and easy to implement without training. The experimental result analysis showed that our method could detect more than 98% of the suspicious liquids, identify more than 97% of the suspicious liquid types, and distinguish up to 94% of the different concentrations of alcohol

Jiedu Tongluo granules ameliorates post-stroke depression rat model via regulating NMDAR/BDNF signaling pathway

Post-stroke depression (PSD) is one of the most common stroke complications, which seriously affects stroke’s therapeutic effect and brings great pain for patients. The pathological mechanism of PSD has not been revealed. Jiedu Tongluo granules (JDTLG) is an effective traditional Chinese medicine for PSD treatment which is widely used in clinical treatment. JDTLG has a significant therapeutic effect against PSD, but the mechanism is still unclear. The PSD rat model was established by carotid artery embolization combined with chronic sleep deprivation followed by treating with JDTLG. Neurobehavioral and neurofunctional experiments were engaged in studying the neural function of rats. Histomorphology, proteomics, and western blotting researches were performed to investigate the potential molecular mechanisms related to JDTLG therapy. Oral treatment of JDTLG could significantly improve the symptoms of neurological deficit and depression symptoms of PSD rats. Proteomic analysis identified several processes that may involve the regulation of JDTLG on the PSD animal model, including energy metabolism, nervous system, and N-methyl-D-aspartate receptor (NMDAR)/brain-derived neurotrophic factor (BDNF) signal pathway. Our results showed that JDTLG could reduce glutamate (Glu) level and increase gamma-aminobutyric acid (GABA) level via regulating the NMDAR/BDNF pathway, which may play a vital role in the occurrence and development of PSD

Securing health monitoring via body-centric time-frequency signature authorization

Identity-based attacks serve as the basis of an intruder’s attempt to launch security infringements in mobile health monitoring scenarios. Wireless channel perturbations due to the presence of human body are a relative phenomenon depending heavily on the subject’s dielectric properties. A new Body-Centric Signature Authorization (B-CSAI) approach based on time-frequency domain characteristics was proposed. This method utilizes multiple millimeter wave bands of 27-28 GHz, 29-30 GHz, and 31-32 GHz, thereby enhancing the security in body-centric communications exploiting benefits of subject specific channel signature. The proposed bornprint method is based on the intrinsic identity related time-frequency domain information, which generated by the user’s natural hand motion signature and resulting creeping waves and space waves. It can meet the unconditional keyless authorization requirements. A detailed measurement campaign considering radiation efficiency (η = -25.8, -24.7, -26.4), pathloss exponent, and shadowing factor in three millimeter wave bands, using six human subjects confirm the usability and efficiency of the proposed approach. This also shows that there is a wide space for realizing security from physical mechanisms