2 research outputs found
Crossed Ga<sub>2</sub>O<sub>3</sub>/SnO<sub>2</sub> Multiwire Architecture: A Local Structure Study with Nanometer Resolution
Crossed nanowire structures are the basis for high-density integration
of a variety of nanodevices. Owing to the critical role of nanowires
intersections in creating hybrid architectures, it has become a challenge
to investigate the local structure in crossing points in metal oxide
nanowires. Thus, if intentionally grown crossed nanowires are well-patterned,
an ideal model to study the junction is formed. By combining electron
and synchrotron beam nanoprobes, we show here experimental evidence
of the role of impurities in the coupling formation, structural modifications,
and atomic site configuration based on crossed Ga<sub>2</sub>O<sub>3</sub>/SnO<sub>2</sub> nanowires. Our experiment opens new avenues
for further local structure studies with both nanometer resolution
and elemental sensitivity
Combined Computed Nanotomography and Nanoscopic X‑ray Fluorescence Imaging of Cobalt Nanoparticles in Caenorhabditis elegans
Synchrotron
radiation phase-contrast computed nanotomography (nano-CT)
and two- and three-dimensional (2D and 3D) nanoscopic X-ray fluorescence (nano-XRF) were used to
investigate the internal distribution of engineered cobalt nanoparticles
(Co NPs) in exposed individuals of the nematode Caenorhabditis
elegans. Whole nematodes and selected tissues and
organs were 3D-rendered: anatomical 3D renderings with 50 nm voxel
size enabled the visualization of spherical nanoparticle aggregates
with size up to 200 nm within intact C. elegans. A 20 × 37 nm<sup>2</sup> high-brilliance beam was employed
to obtain XRF elemental distribution maps of entire nematodes or anatomical
details such as embryos, which could be compared with the CT data.
These maps showed Co NPs to be predominantly present within the intestine
and the epithelium, and they were not colocalized with Zn granules
found in the lysosome-containing vesicles or Fe agglomerates in the
intestine. Iterated XRF scanning of a specimen at 0° and 90°
angles suggested that NP aggregates were translocated into tissues
outside of the intestinal lumen. Virtual slicing by means of 2D XRF
tomography, combined with holotomography, indicated presumable presence
of individual NP aggregates inside the uterus and within embryos