Gene duplication in an African cichlid adaptive radiation

Background Gene duplication is a source of evolutionary innovation and can contribute to the divergence of lineages; however, the relative importance of this process remains to be determined. The explosive divergence of the African cichlid adaptive radiations provides both a model for studying the general role of gene duplication in the divergence of lineages and also an exciting foray into the identification of genomic features that underlie the dramatic phenotypic and ecological diversification in this particular lineage. We present the first genome-wide study of gene duplication in African cichlid fishes, identifying gene duplicates in three species belonging to the Lake Malawi adaptive radiation (Metriaclima estherae, Protomelas similis, Rhamphochromis “chilingali”) and one closely related species from a non-radiated riverine lineage (Astatotilapia tweddlei). Results Using Astatotilapia burtoni as reference, microarray comparative genomic hybridization analysis of 5689 genes reveals 134 duplicated genes among the four cichlid species tested. Between 51 and 55 genes were identified as duplicated in each of the three species from the Lake Malawi radiation, representing a 38%–49% increase in number of duplicated genes relative to the non-radiated lineage (37 genes). Duplicated genes include several that are involved in immune response, ATP metabolism and detoxification. Conclusions These results contribute to our understanding of the abundance and type of gene duplicates present in cichlid fish lineages. The duplicated genes identified in this study provide candidates for the analysis of functional relevance with regard to phenotype and divergence. Comparative sequence analysis of gene duplicates can address the role of positive selection and adaptive evolution by gene duplication, while further study across the phylogenetic range of cichlid radiations (and more generally in other adaptive radiations) will determine whether the patterns of gene duplication seen in this study consistently accompany rapid radiation

Transduction Mechanisms and Power Density for MEMS Inertial Energy Scavengers

Accepted versio

L\'evy Distribution of Single Molecule Line Shape Cumulants in Low Temperature Glass

We investigate the distribution of single molecule line shape cumulants, $\kappa_1,\kappa_2,...$, in low temperature glasses based on the sudden jump, standard tunneling model. We find that the cumulants are described by L\'evy stable laws, thus generalized central limit theorem is applicable for this problem.Comment: 5 pages, 3 figure

European expectations of disaster information provided by critical infrastructure operators

Previous research into social media crisis communication has tended to focus on use by emergency managers rather than other key stakeholder, critical infrastructure (CI) operators. This article adds to the field by empirically investigating public expectations of information provided by CI operators during crisis situations and if CI operators currently meet such expectations. It draws on key themes that emerged from a review of the literature on public expectations of disaster related information shared via social media. Then, it presents the results of an online questionnaire and interview-based study of disaster-vulnerable communities in France, Norway, Portugal and Sweden. Results indicate that members of the public expect CI operators to provide disaster related information via traditional and social media, but not necessarily respond to their queries on social media. Operators appear to meet public expectations of traditional media use, but should expand their current practices to include digital media. Recommendations for CI operators on how to do use social media follow

Local origins impart conserved bone type-related differences in human osteoblast behaviour

Osteogenic behaviour of osteoblasts from trabecular, cortical and subchondral bone were examined to determine any bone type-selective differences in samples from both osteoarthritic (OA) and osteoporotic (OP) patients. Cell growth, differentiation; alkaline phosphatase (TNAP) mRNA and activity, Runt-related transcription factor-2 (RUNX2), SP7-transcription factor (SP7), bone sialoprotein-II (BSP-II), osteocalcin/bone gamma-carboxyglutamate (BGLAP), osteoprotegerin (OPG, TNFRSF11B), receptor activator of nuclear factor-κβ ligand (RANKL, TNFSF11) mRNA levels and proangiogenic vascular endothelial growth factor-A (VEGF-A) mRNA and protein release were assessed in osteoblasts from paired humeral head samples from age-matched, human OA/OP (n = 5/4) patients. Initial outgrowth and increase in cell number were significantly faster (p < 0.01) in subchondral and cortical than trabecular osteoblasts, in OA and OP, and this bone type-related differences were conserved despite consistently faster growth in OA. RUNX2/SP7 levels and TNAP mRNA and protein activity were, however, greater in trabecular than subchondral and cortical osteoblasts in OA and OP. BSP-II levels were significantly greater in trabecular and lowest in cortical osteoblasts in both OA and OP. In contrast, BGLAP levels showed divergent bone type-selective behaviour; highest in osteoblasts from subchondral origins in OA and trabecular origins in OP. We found virtually identical bone type-related differences, however, in TNFRSF11B:TNFSF11 in OA and OP, consistent with greater potential for paracrine effects on osteoclasts in trabecular osteoblasts. Subchondral osteoblasts (OA) exhibited highest VEGF-A mRNA levels and release. Our data indicate that human osteoblasts in trabecular, subchondral and cortical bone have inherent, programmed diversity, with specific bone type-related differences in growth, differentiation and pro-angiogenic potential in vitro

Selected Hydrogeologic and Water-quality Data from Jones Beach Island, Long Island, New York

A data-collection site was instrumented on Jones Beach Island, a barrier island south of Long Island, N.Y., to study local freshwater/ saltwater relations in the shallow ground-water system. A geologic test boring revealed about 88 feet of well-sorted glacial outwash sand above about 15 feet of Gardiners Clay, which directly overlies silty sand of the Magothy Formation. Tidal effects on water levels in Great South Bay, the upper glacial aquifer, and the Magothy aquifer were observed and quantified with a tidal gage in the bay and analog water-level recorders in the wells.Chloride concentrations in the upper Magothy aquifer were higher than expected--about 270 mg/L (milligrams per liter), and those in the upper glacial aquifer were 17,000 to 19,000 mg/L, about the same as in Great South Bay. Estimates of pressure and freshwater equivalent heads indicate that, at the data-collection site, freshwater is discharging upward from the Magothy aquifer into the salty upper glacial aquifer, but dilution by this freshwater is undetectable. The reason for the elevated chloride concentration in the Magothy aquifer cannot be determined from available hydrogeologic information

The Relationship between Emotion Dysregulation and Postnatal Attachment in Women Admitted to a Mother Baby Unit

Mothers with insecure attachment styles may have increased difficulty establishing secure attachment with their infant and may experience emotion regulation difficulties in the postpartum period. This study aimed to examine the mediating effect of emotion dysregulation (ED) on the relation-ship between maternal insecure attachment style and self-reported attachment to infant in women admitted to a mother-baby unit

Brachial plexopathy after prone positioning

Two cases of brachial plexus injury after prone position in the intensive care unit are described. Mechanisms of brachial plexus injury are described, as are methods for prevention of this unusual complication

Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger faeces. Immunomagnetic capture, immunofluorescence and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle