Muscular Lesions Induced By A Hemorrhagic Factor From Bothrops Neuwiedi Snake Venom

The local tissue effects of crude Bothrops neuwiedi snake venom and of its hemorrhagic factor (NHF) were studied on mouse tibialis anterior muscle in vivo. After 6 h, 8 days and 6 weeks the muscles were examined in paraffin sections stained with hematoxylin and eosin. Both NHF and crude venom produced hemorrhage and myonecrosis, later followed by muscle fiber regeneration. Intramuscular arteries also suffered necrosis. The minimal dose of NHF necessary to produce detectable hemorrhage and myonecrosis was 50 ng, while the minimal venom dose needed to produce the same effect was 20 times higher. The results indicate that NHF is one of the major factors responsible for the local effects of B. neuwiedi venom.18333734

Pathological Changes In Muscle Caused By Haemorrhagic And Proteolytic Factors From Bothrops Jararaca Snake Venom

Haemorrhagic factor HF2 and bothropasin, two metalloproteins isolated from the venom of Bothrops jararaca, caused haemorrhage followed by myonecrosis and arterial necrosis after i.m. injection in mice. The effects of HF2 were qualitatively similar to those of bothropasin and crude B. jararaca venom, but its potency was about 20 times higher. The haemorrhagic and necrotizing actions of these components are unrelated to their proteolytic activity on casein. © 1985.232341345NSERC; Natural Sciences and Engineering Research Council of CanadaBrazil, (1914) La Défense Contre l'Ophidisme, , 2nd Edn., Pocai & Weiss, São PauloHoussay, Acción local de los venenos de serpientes (1923) Revta Asoc. méd. Argent., 36, p. 39de Lacerda, (1881) Provas Experimentaes de que a Peçonha das Cobras é um Succo Digestivo, , Museu Nacional, Rio de JaneiroMandelbaum, Reichl, Assakura, Some physical and biochemical characteristics of HF2, one of the hemorrhagic factors in the venom of Bothrops jararaca (1976) Animal, Plant and Microbial Toxins, 1, p. 111. , A. Ohsaka, K. Hayashi, Y. Saway, Plenum Press, LondonMandelbaum, Reichl, Assakura, Isolation and characterization of a proteolytic enzyme from the venom of the snake Bothrops jararaca (jararaca) (1982) Toxicon, 20, p. 955Ownby, Bjarnason, Tu, Hemorrhagic toxins from rattlesnake (Crotalus atrox) venom (1978) Pathogenesis of hemorrhage induced by three purified toxins, 93, p. 201. , Am. J. PathQueiroz, Santo Neto, Rodrigues-Simioni, Prado-Franceschi, Muscle necrosis and regeneration after envenomation by Bothrops jararacussu snake venom (1984) Toxicon, 22, p. 339Queiroz, Santo Neto, Brandeburgo, Simioni, Giglio, Histopathological changes caused by a myotoxin from Bothrops jararacussu snake venom (1985) Comparison with the effects of whole venom. Abstract, 23, p. 17. , Toxico