Exploring deep microbial life in coal-bearing sediment down to ~2.5 km below the ocean floor

Microbial life inhabits deeply buried marine sediments, but the extent of this vast ecosystem remains poorly constrained. Here we provide evidence for the existence of microbial communities in ~40° to 60°C sediment associated with lignite coal beds at ~1.5 to 2.5 km below the seafloor in the Pacific Ocean off Japan. Microbial methanogenesis was indicated by the isotopic compositions of methane and carbon dioxide, biomarkers, cultivation data, and gas compositions. Concentrations of indigenous microbial cells below 1.5 km ranged from <10 to ~10^4 cells cm^(−3). Peak concentrations occurred in lignite layers, where communities differed markedly from shallower subseafloor communities and instead resembled organotrophic communities in forest soils. This suggests that terrigenous sediments retain indigenous community members tens of millions of years after burial in the seabed

Repellency Assessment of Nepeta cataria Essential Oils and Isolated Nepetalactones on Aedes aegypti.

There is an increased need for improved and affordable insect repellents to reduce transmission of rapidly spreading diseases with high mortality rates. Natural products are often used when DEET cannot be afforded or accessed and when consumers choose not to use a synthetic repellent. The essential oils from two newly bred Nepeta cataria (catnip) plants representing two different chemotypes and their respective isolated nepetalactone isomers were evaluated as mosquito repellents against Aedes aegypti mosquitoes that transmit the Zika and Dengue virus in a one choice landing rate inhibition assay. A dose response curve was generated for each treatment and a time course analysis of repellency was performed over 24 hours with a N. cataria essential oil sample. The results indicate that all essential oil samples and their respective purified nepetalactone isomers were able to achieve greater than 95% repellency. Between two and four hours, the ability to repel more than 95% of the mosquitoes diminished. At the lowest concentrations tested, the nepetalactones and crude essential oil samples were more effective than DEET at reducing the number of mosquito landings

A search for X-ray emission from rich clusters, extended halos around clusters, and superclusters

The all-sky data base acquired with the HEAO A-2 experiment was searched for X-ray emission on a variety of metagalactic size scales which were either predicted or previously detected. Results in the 0.2-60 keV energy range are presented. The optically richest clusters, including those from which a microwave decrement were observed, appear to be relatively underluminous in X-rays. Observations of Abell 576 show its luminosity to be less than earlier estimates, and moreover less than the luminosity predicted from its microwave decrement, unless the intracluster gas is a factor of approximately 10 hotter than in typical clusters. Near SC0627 there are two X-ray sources, and the identification of the dominant source with SCO627 is probably incorrect. New spectral observations of Abell 401 and 2147, possible superclusters, reveal that they have typical cluster spectra with iron line emission

How Does Peer Honesty Affect Focal Manager Honesty in a Budget Reporting Setting?

We examine the effect of peer honesty on focal manager honesty in a budget reporting setting. We disclose peer honesty to the focal manager at three levels: no, partial, and full disclosure of the reporting behavior of the other managers in the focal managers’ cohort. In partial disclosure, only the reports of the least honest peers are disclosed to the focal manager. In full disclosure, all managers’ reports in the cohort are disclosed to the focal manager. We predict and find that disclosure of other managers’ reports leads to less honesty compared to the absence of disclosure. We show that disclosure changes the focal manager’s perceptions of what constitutes acceptable reporting behavior, such that reporting more dishonestly becomes more acceptable. Our results have implications for understanding fraud dynamics and have practical implications for the design of control systems, as they suggest that managers will use peer dishonesty to justify their own dishonesty, even when they know that only some of their peers report dishonestly

Eliciting density ratio classes

AbstractThe probability distributions of uncertain quantities needed for predictive modelling and decision support are frequently elicited from subject matter experts. However, experts are often uncertain about quantifying their beliefs using precise probability distributions. Therefore, it seems natural to describe their uncertain beliefs using sets of probability distributions. There are various possible structures, or classes, for defining set membership of continuous random variables. The Density Ratio Class has desirable properties, but there is no established procedure for eliciting this class. Thus, we propose a method for constructing Density Ratio Classes that builds on conventional quantile or probability elicitation, but allows the expert to state intervals for these quantities. Parametric shape functions, ideally also suggested by the expert, are then used to bound the nonparametric set of shapes of densities that belong to the class and are compatible with the stated intervals. This leads to a natural metric for the size of the class based on the ratio of the total areas under upper and lower bounding shape functions. This ratio will be determined by the characteristics of the shape functions, the scatter of the elicited values, and the explicit expert imprecision, as characterized by the width of the stated intervals. We provide some examples, both didactic and real, and conclude with recommendations for the further development and application of the Density Ratio Class

Challenges in Exosome Isolation and Analysis in Health and Disease

A growing body of evidence emphasizes the important role exosomes in different physiological and pathological conditions. Exosomes, virus-size extracellular vesicles (EVs), carry a complex molecular cargo, which is actively processed in the endocytic compartment of parental cells. Exosomes carry and deliver this cargo to recipient cells, serving as an intercellular communication system. The methods for recovery of exosomes from supernatants of cell lines or body fluids are not uniformly established. Yet, studies of the quality and quantity of exosome cargos underlie the concept of “liquid biopsy.” Exosomes are emerging as a potentially useful diagnostic tool and a predictor of disease progression, response to therapy and overall survival. Although many novel approaches to exosome isolation and analysis of their cargos have been introduced, the role of exosomes as diagnostic or prognostic biomarkers of disease remains unconfirmed. This review considers existing challenges to exosome validation as disease biomarkers. Focusing on advantages and limitations of methods for exosome isolation and characterization, approaches are proposed to facilitate further progress in the development of exosomes as biomarkers in human disease

Pan-European grading scales: lessons from national systems and the ECTS

This article assesses the impact of the Bologna Process on the grading schemes of EU member countries. In light of some problems regarding the implementation of the European Credit Transfer system (ECTS), the author proposes further reforms and offers some elements of a unified grading system for European higher education. The author explores the variation among Europe’s grading systems and the resulting lessons learned are shared here. Lastly, this article also argues that principles of justice and fairness, deemed central to academic freedom, are best upheld by the use of a unified grading system at national and European levels