235 research outputs found
La introducción del euro y las estabilizaciones jurÃdicas de las expectativas de los consumidores
La protección de los consumidores en los contratos de servicios según el derecho comunitario
Recent Trends in European Legal Education: The Place of the European Law Faculties Association
Recent Trends in European Legal Education: The Place of the European Law Faculties Association
Transformation of Contract Law and Civil Justice in the New EU Member Countries - The Example of the Baltic States, Hungary and Poland
Transformation of Contract Law and Civil Justice in the New EU Member Countries - The Example of the Baltic States, Hungary and Poland
Differential stabilization of reaction intermediates: specificity checkpoints for M.EcoRI revealed by transient fluorescence and fluorescence lifetime studies
M.EcoRI, a bacterial sequence-specific S-adenosyl-l-methionine-dependent DNA methyltransferase, relies on a complex conformational mechanism to achieve its remarkable specificity, including DNA bending, base flipping and intercalation into the DNA. Using transient fluorescence and fluorescence lifetime studies with cognate and noncognate DNA, we have characterized several reaction intermediates involving the WT enzyme. Similar studies with a bending-impaired, enhanced-specificity M.EcoRI mutant show minimal differences with the cognate DNA, but significant differences with noncognate DNA. These results provide a plausible explanation of the way in which destabilization of reaction intermediates can lead to changes in substrate specificity
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