Investigation on the Effect of Chemical and Biological Control of Bacterial Soft Root Disease of Potato in Storage.

Potato soft rot, caused by Erwiniacarotovora subsp. carotovora, greatly affect potato tuber quality in storage of Egypt and indicated that it can affect all potato cultivars. Erwiniacarotovora subsp. carotovora was isolated from the infected potato tuber and was identified by pathological, morphological and biochemical studies.  One antimicrobialchemical compound chitosans (CS) with concentration (1, 3, 5 %) combine with three biocontrol agents (Bacillus subtilis, Pseudomonas fluorescens and Trichodermavirdi) each. These treatments were screened out in vitro and in storage against the growth of Erwinia carotovora subsp carotovora. All bio-control agents combined with Cs reduced the bacterial soft rot disease to various degrees. The stronger antagonistic activity against Erwinia carotovora was found in treatment Cs 5% with biocontrolagaints (T. virdi, P. fluorescens and B. subtilis respectively). All treatments reduced the soft rot infection to 20-week storage with two types of potatoes (Spunta and Cara) varieties by (91, 86, 83.6 and 77.3% respectively in Spunta c.v.) and (88.6, 86.4 and 79.8% in Cara c.v.). The lowest antagonistic activity against Erwinia carotovora was found in treatment CS 1% with biocontrol agents (T. virdi, P. fluorescens and B. subtilis respectively) varieties by (64.2, 58.6 and 43.7% respectively) compared with the treatments of biocontrol agents individually. Keywords: Potato - Erwiniacarotovora- Chitosan- T. virdi - P. fluorescens- Bacillus subtili

Some Properties of Chaotic Modified of Bogdanov Map

In this  research to the modified dynamics of Bogdanov's map  studied, and the  found sensitivity to the initial conditions of the modified map  found as well as the Lyapunov exponent .the general characteristics of the map  by the diffeomorpism. Finally we boosted my research  with matlab to find chaotic area

Influence of preharvest spraying with the chemical salts on grey mold disease and keeping quality of table grapes during storage

The efficacy of calcium chloride, calcium nitrate, potassium metabisulfate, calcium carbonate and sodium bicarbonate to protect grape bunches during harvesting was studied under storage conditions. This experiment was carried out in a private vineyard, El-Khatatba region, Alexandria desert road, Egypt during 2014 and 2015 growing seasons on Thompson seedless variety. The chemical used at 0.1 and 0.2% g /liter water. The healthy grape bunches of all treatments were harvested at proper ripeness, packed in perforated plastic bags, put inside carton boxes and stored at 0oC under 90-95% R.H. for 36 days. Other grape bunches either treated or untreated with the salts were artificially inoculated with spore suspension of Botrytis cinerea at a concentration of 3x106 spores/ml before packing and cold storage. Disease severity of grape bunches with B. cinerea was estimated after 36 days of cold storage. All treatments exhibited disease reduction under field conditions of Thompson seedless grape variety. The field applications of salts gave a significant reduction of botrytis rot during storage. In this respect, the grape rots were significantly reduced when sprayed by the salts three times

SYNTHESIS, ANTITUMOR ACTIVITY, PHARMACOPHORE MODELING AND QSAR STUDIES OF NOVEL PYRAZOLES AND PYRAZOLO [1, 5-A] PYRIMIDINES AGAINST BREAST ADENOCARCINOMA MCF-7 CELL LINE

Objective: The present work aimed to synthesize New series of pyrazoles 3 and pyrazolo[1,5-a]pyrimidines 5, 7, 9 in order to evaluate their antiproliferative activity against human breast adenocarcinoma MCF-7cell line and study the cell cycle progression of the most active compounds. In addition, Pharmacophore modeling and QSAR Studies of these new compounds were done.Methods: The diazonium salt of 4-aminoacetophenone 1 was coupled with malononitrile in ethanol using sodium acetate affords 2-[(4-acetylphenyl)diazenyl] malononitrile Cycloaddition of hydrazine hydrate, in molar ratios 1:1 or 1:2, on compound 2, furnished 3,5-diaminopyrazolederivatives 3a and 3b respectively. Moreover, new pyrazolo[1,5-a]pyrimidine derivatives 5a-f were obtained upon cyclocondensation of 3a, b with different chalcones 4a-c in EtOH/piperidine,while compounds 7a-f were prepared via cycloaddition of 3a, b with various arylidene malononitriles 6a-c in the same reaction condition. Finally, treatment of 3a, b with ethyl 2-cyano-3-ethoxyacrylate 8a or 2-(ethoxymethylene)malononitrile 8b in EtOH/TEA yielded the novel pyrazolo[1,5-a]pyrimidine derivatives 9a, b respectively. These target compounds were screened for their cytotoxic activity against MCF-7 (human breast Cell Line) followed by study cell cycle of 7a. Finally, Pharmacophore modeling and QSAR Studies was carried out.Results: The pyrazolopyrimidine 7a was the most active compound (IC50 = 3.25 µM), whereas, some of the tested compounds exploited moderate growth inhibitory activity. Its effect was further studied on cell cycle progression; results showed that compound 7a induced cell cycle arrest at S-phase verifying this compound as a promising selective anticancer agent.Conclusion: Compound 7a was found to be the most active member against MCF-7 breast cancer (IC50= 3.25 μM), Further biological assessment of 7a using flow-cytometric analysis, revealed that it induced cell cycle arrest at S phase.Keywords: Pyrazole, Pyrazolo[1,5-a]pyrimidine, MCF-7 breast cancer cell line, Cell cycle profile, 3D pharmacophore,1 QSAR stud

OPTIMUM OPERATION MANAGEMENT EFFECT OF MAIN SEWAGE PUMPING STATIONS ON TRUNK SEWER DETERIORATION

Sewage pumping stations are considered an important part of any sewerage system. Pumps failure in these stations means that the pumps are unable to work at the design requirement (flow capacity and head) and that may cause sewer overflow and flooding leading to sewer deterioration. In this paper, two main sewage pumping stations in Baghdad city were selected as case studies, AlHabibia and Al-Ghazali located on Zublin trunk sewer 3000 mm and Baghdad trunk sewer 1200-2100 respectively. This study focused mainly on the operation of main sewage pumping stations and their effect, both directly and indirectly, on changing hydraulic properties, which leads to an increase in the deterioration of sewage pipes. The hydraulic analysis was conducted to investigate the effect of the operational performance of these stations on the deterioration of the trunk sewers. In general, the sewage pumps are in good condition based on the completed evaluation of these stations but, it was observed that Al-Habibia sewage pumping station was unable to discharge high sewage flow (d/D = 0.75). Backup flow occurred in the inlet sewer and caused overflow and flooding. The hydraulic analysis for the effluent sewer showed that the actual flowing velocities of the operating conditions examined were greater than the minimum selfcleaning velocity. Al-Ghazali sewage pumping station was able to receive the influent sewage for all the cases of flow (low, medium and high). While the effluent sewer (Baghdad trunk sewer) that was 70% filled with sediments, that reduced its capacity, does not work with the high discharges of the pumps. Therefore, resulting in sewage flows from this overloaded sewer onto the streets and harms the environment

Distributions of Generalized Order Statistics and Parameters Estimation of Pareto Distribution in Statistical Explicit Forms

Abstract : We study some distributions of generalized order statistics (GOS) for Pareto distribution. In particular, we have derived the joint probability density function (pdf) for GOS from Pareto distribution in statistical explicit form. In addition, the joint pdf of both range and midrange for GOS from Pareto distribution is obtained. The estimation of Pareto distribution parameters based on GOS using the method of Maximum Likelihood Estimators (MLE) have been derived in explicit forms. Furthermore, some special cases have been discussed. Keywords Pareto Distribution, Generalized Order Statistics, Ordinary Order Statistics, Maximum Likelihood Estimation

A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

<p>Abstract</p> <p>Background</p> <p>Crimean-Congo hemorrhagic fever (CCHF), a tick-borne disease caused by Crimean-Congo hemorrhagic fever virus (CCHFV), is a member of the genus Nairovirus in the family Bunyaviridae. Recently, CCHFV has been reported as an important emerging infectious viral pathogen in Sudan. Sporadic cases and multiple CCHF outbreaks, associated with nosocomial chain of transmission, have been reported in the Kordufan region of Sudan.</p> <p>Aims</p> <p>To confirm CCHF in an index patient and attending physician in North Kordufan region, Sudan, and to provide some information on virus genetic lineages.</p> <p>Methods</p> <p>Antibody captured ELISA, reverse transcription PCR, partial S segment sequences of the virus and subsequent phylogenetic analysis were used to confirm the CCHFV infection and to determine the virus genetic lineages.</p> <p>Results</p> <p>CCHF was confirmed by monitoring specific IgM antibody and by detection of the viral genome using RT-PCR. Treatment with oral ribavirin, replacement with fluid therapy, blood transfusion and administration of platelets concentrate resulted in rapid improvement of the health condition of the female physician. Phylogenetic analysis of the partial S segment sequences of the 2 CCHFV indicates that both strains are identical and belong to Group III virus lineage, which includes viruses from Africa including, Sudan, Mauritania, South Africa and Nigeria.</p> <p>Conclusion</p> <p>Further epidemiologic studies including, CCHFV complete genome analysis and implementation of improved surveillance are urgently needed to better predict and respond to CCHF outbreaks in the Kordufan region, Sudan.</p

The impact of sequencing depth on the inferred taxonomic composition and AMR gene content of metagenomic samples

Shotgun metagenomics is increasingly used to characterise microbial communities, particularly for the investigation of antimicrobial resistance (AMR) in different animal and environmental contexts. There are many different approaches for inferring the taxonomic composition and AMR gene content of complex community samples from shotgun metagenomic data, but there has been little work establishing the optimum sequencing depth, data processing and analysis methods for these samples. In this study we used shotgun metagenomics and sequencing of cultured isolates from the same samples to address these issues. We sampled three potential environmental AMR gene reservoirs (pig caeca, river sediment, effluent) and sequenced samples with shotgun metagenomics at high depth (~ 200 million reads per sample). Alongside this, we cultured single-colony isolates of Enterobacteriaceae from the same samples and used hybrid sequencing (short- and long-reads) to create high- quality assemblies for comparison to the metagenomic data. To automate data processing, we developed an open- source software pipeline, ‘ResPipe’