Nowe stanowiska Pyrrhocoris marginatus (Kolenati, 1845) (Heteroptera: Pyrrhocoridae) w Polsce

[Nowe lokalizacje Pyrrhocoris marginatus (Kolenati, 1845) (Heteroptera: Pyrrhocoridae) w Polsce]. Pyrrhocoris marginatus do tej pory znany był w Polsce tylko z dwóch dokładnych lokalizacji. Notatka przedstawia trzy nowe stanowiska tego gatunku z Górny i Dolny Śląsk

Przyczynek do rozmieszczenia pluskwiaków różnoskrzydłych (Hemiptera: Heteroptera) w Polsce - III

[A contribution to the distribution of true bugs (Hemiptera: Heteroptera) in Poland – III]. This paper is a continuation of a series of publications on the distribution of true bugs in Poland and includes new faunistic data for 306 species of true bugs. In total, data on 62 species previously unreported in 21 zoogeographical regions of Poland is presented, including some rarely collected: Brachyarthrum limitatum, Eurydema fieberi, Peritrechus gracilicornis, Stephanitis pyri, Tingis crispata. It is also noteworthy that this paper was largely prepared using citizen science, where many people (non-specialists in Heteroptera) collected data constituting almost 25% of the presented records. Importantly, the true bugs recorded in this way include species very rarely collected in Poland, and species alien to Polish fauna (e.g. Oxycarenus lavaterae, Nezara viridula and Halyomorpha halys). Due to the lack of funding being a significant obstacle to biodiversity studies in Poland, citizen science seems to be the only way to effectively monitor all the dynamic changes taking place in national entomofauna

Przyczynek do rozmieszczenia pluskwiaków różnoskrzydłych (Hemiptera: Heteroptera) w Polsce

This paper presents almost 1600 new faunistic records for 271 species of Polish Heteroptera. List of species reported for the first time from each zoogeograph-ical region is also provided

Nowe stanowiska rzadkich i ciekawych gatunków pluskwiaków różnoskrzydłych (Hemiptera: Heteroptera) w Polsce

New records of true-bugs interesting and rarely collected in Poland are presented, i.e. Actinonotus pulcher (Herrich-Schaeffer, 1835) – the third record in Western Beskidy, Aradus truncatus Fieber, 1860 - new to Upper Silesia, Ceraleptus gracilicornis (Herrich-Schaeffer, 1835) – the second record in Eastern Beskidy, Chilacis typhae (Perris, 1857) - new to Kraków-Wieluń Upland, Derae-ocoris flavilinea (A. Costa, 1862) - new to Lower Silesia, Deraeocoris olivaceus (Fabricius, 1777) - new to Western Beskidy, Empicoris culiciformis (De Geer, 1773) – the second record in Wielkopolsko-Kujawska Lowland, Heterogaster urticae (Fab-ricius, 1775) - new to Western Beskidy and Baltic Coast, Menaccarus arenicola (Scholtz, 1847) – the second locality in Małopol-ska Upland, Orsillus depressus (Mulsant & Rey, 1852) - new to Western Beskidy, Kraków-Wieluń Upland, and Małopolska Up-land, Oxycarenus lavaterae (Fabricius, 1787) – the second and third records in Poland, Oxycarenus pallens (Herrich-Schaeffer, 1850) – the third record in Western Beskidy, Pinthaeus sanguinipes (Fabricius, 1781), Psallus assimilis Stichel, 1956 – the sec-ond locality in Poland, Pyrrhocoris marginatus (Kolenati, 1845) - new to Małopolska Upland, Tropidothorax leucopterus (Goeze, 1778) – the sixth record in Poland

Identification of Yersinia enterocolitica isolates from humans, pigs and wild boars by MALDI TOF MS

Abstract Background Yersinia enterocolitica is widespread within the humans, pigs and wild boars. The low isolation rate of Y. enterocolitica from food or environmental and clinical samples may be caused by limited sensitivity of culture methods. The main goal of present study was identification of presumptive Y. enterocolitica isolates using MALDI TOF MS. The identification of isolates may be difficult due to variability of bacterial strains in terms of biochemical characteristics. This work emphasizes the necessity of use of multiple methods for zoonotic Y. enterocolitica identification. Results Identification of Y. enterocolitica isolates was based on MALDI TOF MS, and verified by VITEK® 2 Compact and PCR. There were no discrepancies in identification of all human’ and pig’ isolates using MALDI TOF MS and VITEK® 2 Compact. However three isolates from wild boars were not decisively confirmed as Y. enterocolitica. MALDI TOF MS has identified the wild boar’ isolates designated as 3dz, 4dz, 8dz as Y. enterocolitica with a high score of matching with the reference spectra of MALDI Biotyper. In turn, VITEK® 2 Compact identified 3dz and 8dz as Y. kristensenii, and isolate 4dz as Y. enterocolitica. The PCR for Y. enterocolitica 16S rDNA for these three isolates was negative, but the 16S rDNA sequence analysis identified these isolates as Y. kristensenii (3dz, 4dz) and Y. pekkanenii (8dz). The wild boar’ isolates 3dz, 4dz and 8dz could not be classified using biotyping. The main bioserotype present within pigs and human faeces was 4/O:3. It has been shown that Y. enterocolitica 1B/O:8 can be isolated from human faeces using ITC/CIN culturing. Conclusion The results of our study indicate wild boars as a reservoir of new and atypical strains of Yersinia, for which protein and biochemical profiles are not included in the MALDI Biotyper or VITEK® 2 Compact databases. Pigs in the south-west Poland are the reservoir for pathogenic Y. enterocolitica strains. Four biochemical features included in VITEK® 2 Compact known to be common with Wauters scheme were shown to produce incompatible results, thus VITEK® 2 Compact cannot be applied in biotyping of Y. enterocolitica