Age-dependent associations between telomere length and environmental conditions in roe deer

Supplementary methods, tables & figure

Coexistence of two sympatric cryptic bat species in French Guiana: insights from genetic, acoustic and ecological data

International audienceBackground: The distinction between lineages of neotropical bats from the Pteronotus parnellii species complex has been previously made according to mitochondrial DNA, and especially morphology and acoustics, in order to separate them into two species. In these studies, either sample sizes were too low when genetic and acoustic or morphological data were gathered on the same individuals, or genetic and other data were collected on different individuals. In this study, we intensively sampled bats in 4 caves and combined all approaches in order to analyse genetic, morphologic, and acoustic divergence between these lineages that live in the same caves in French Guiana

Characterization of Macrophages and Osteoclasts in the Osteosarcoma Tumor Microenvironment at Diagnosis: New Perspective for Osteosarcoma Treatment?

Biological and histopathological techniques identified osteoclasts and macrophages as targets of zoledronic acid (ZA), a therapeutic agent that was detrimental for patients in the French OS2006 trial. Conventional and multiplex immunohistochemistry of microenvironmental and OS cells were performed on biopsies of 124 OS2006 patients and 17 surgical (“OSNew”) biopsies respectively. CSF-1R (common osteoclast/macrophage progenitor) and TRAP (osteoclast activity) levels in serum of 108 patients were correlated to response to chemotherapy and to prognosis. TRAP levels at surgery and at the end of the protocol were significantly lower in ZA+ than ZA− patients (padj = 0.0011; 0.0132). For ZA+-patients, an increase in the CSF-1R level between diagnosis and surgery and a high TRAP level in the serum at biopsy were associated with a better response to chemotherapy (p = 0.0091; p = 0.0251). At diagnosis, high CD163+ was associated with good prognosis, while low TRAP activity was associated with better overall survival in ZA− patients only. Multiplex immunohistochemistry demonstrated remarkable bipotent CD68+/CD163+ macrophages, homogeneously distributed throughout OS regions, aside osteoclasts (CD68+/CD163−) mostly residing in osteolytic territories and osteoid-matrix-associated CD68−/CD163+ macrophages. We demonstrate that ZA not only acts on harmful osteoclasts but also on protective macrophages, and hypothesize that the bipotent CD68+/CD163+ macrophages might present novel therapeutic targets

Spatial Multicomponent Agent-Based Simulation of a Fishery

International audienc

Spatial Multicomponent Agent-Based Simulation of a Fishery

International audienc

Agent-Based Modelling of the spread of COVID-19 in Corsica

International audienc

Leaf microbiome data for European beech (Fagus sylvatica) at the leaf and canopy scales collected in a gallery forest in South-West France

Key message The datasets describe bacterial and fungal communities of European beech ( Fagus sylvatica ) leaves collected along a vertical gradient in a gallery forest throughout the growing season. They also describe communities in the surrounding environment of beech trees. Dataset access is at https://doi.org/10.7910/DVN/FFHAQU , and associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/f17fe848-fc3e-4297-be11-9871b35a1be4 . Both can be used to uncover the dynamics and assembly processes of phyllosphere microbial communities in forest ecosystems.CEnter of the study of Biodiversity in AmazoniaCOntinental To coastal Ecosystems: evolution, adaptability and governanceBiosurveillance Next-Gen des changements dans la structure et le fonctionnement des écosystème

Initial bacterial retention on polydimethylsiloxane of various stiffnesses: The relevance of modulus (mis)match

International audienceInitial retention of the bacterium Escherichia coli on model poly(dimethylsiloxane) (PDMS) surfaces was studied as a function of substrate bulk and surface mechanical stiffness values. Our reference PDMS system was designed such that out of the parameters that govern bacterial adhesion only the mechanical stiffness was systematically varied. This was achieved by varying the crosslinking density of PDMS. Following crosslinking, we performed Soxhlet extraction of non-crosslinked, free chains to rule out their effect on bacterial response. Bulk moduli were assessed by dynamic mechanical analysis at 1 rad sec−1 frequency and the values obtained ranged between 0.03 and 1.8 MPa. The increase in crosslink density resulted in increasing surface modulus, as measured by atomic force microscopy, with values ranging between 0.7 and 9 MPa. The number of bacteria retained was then assessed. We observed a decreasing trend with the increase of both bulk and surface mechanical stiffnesses down to a limit corresponding to the Young's modulus of the bacterial cell surface. For higher values than this threshold, the number of retained bacteria remained constant. We tentatively explain this observation by considering conformal overlay of bacterial and material surfaces

Effect of carbon content on structural, mechanical and tribological properties of Cr-V-C-N coatings

Cr-V-C-N thin films were deposited on XC100 steel and Si(100) wafers by a radio frequency magnetron sputtering technique using chromium and vanadium targets in an Ar/N2/CH4 mixture atmosphere. The microstructure, mechanical and tribological properties of coatings were investigated as a function of carbon content. It has been found that the quaternary Cr-V-C-N coatings containing a low percentage of carbon (≤ 12.4 at.%) exhibited a mixture of chromium and vanadium nitrides nano-sized crystallite phases. The coatings containing a high carbon content (> 25 at.%) were consisted of nitride and carbide phases, where the large carbon atoms inserted through CrN and VN. Mechanical properties of the Cr-V-C-N coatings were influenced by the carbon addition. The maximum hardness value of 28.3 GPa was obtained for the coating containing 28 at.% of carbon which is related to the adhesion strength enhanced by the formation of carbide and nitride mixture. Addition of carbon into the Cr-V-N coating led to significantly decrease its friction coefficient from 0.63 to 0.47. The formation of carbides through the dispersion of carbon in the grains effectively improved the density of the Cr-V-C-N coatings so that the coating deposited under a high CH4 flow rate exhibited a better wear resistance than the other Cr-V-N and Cr-V-C coatings

Tumor necrosis factor receptor associated factor 4 (TRAF4) expression pattern during mouse development

International audienceThis is the first in situ hybridization analysis of expression of a tumor necrosis factor (TNF) receptor associated factor (TRAF) during development. TRAF4 is observed throughout mouse embryogenesis, most notably during ontogenesis of the central (CNS) and peripheral (PNS) nervous system, and of nervous tissues of sensory organs. TRAF4 is preferentially expressed by post-mitotic undifferentiated neurons. Interestingly, TRAF4 remains expressed in the adult hippocampus and olfactory bulb, known to contain multipotential cells responsible for neoneurogenesis