Host Nectin-1 Promotes Chlamydial Infection in the Female Mouse Genital Tract, But is Not Required for Infection in a Novel Male Murine Rectal Infection Model

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen, but more than 70% of patients fail to seek treatment due to the asymptomatic nature of these infections. Women suffer from numerous complications from chronic chlamydial infections, which include pelvic inflammatory disease and infertility. We previously demonstrated in culture that host cell nectin-1 knockdown significantly reduced chlamydial titers and inclusion size. Here, we sought to determine whether nectin-1 was required for chlamydial development in vivo by intravaginally infecting nectin-1-/- mice with Chlamydia muridarum and monitoring chlamydial shedding by chlamydial titer assay. We observed a significant reduction in chlamydial shedding in female nectin-1-/- mice compared to nectin-1+/+ control mice, an observation that was confirmed by PCR. Immunohistochemical staining in mouse cervical tissue confirmed that there are fewer chlamydial inclusions in Chlamydia-infected nectin-1-/- mice. Notably, anorectal chlamydial infections are becoming a substantial health burden, though little is known regarding the pathogenesis of these infections. We therefore established a novel male murine model of rectal chlamydial infection, which we used to determine whether nectin-1 is required for anorectal chlamydial infection in male mice. In contrast to the data from vaginal infection, no difference in rectal chlamydial shedding was observed when male nectin-1+/+ and nectin-1-/- mice were compared. Through the use of these two models, we have demonstrated that nectin-1 promotes chlamydial infection in the female genital tract but does not appear to contribute to rectal infection in male mice. These models could be used to further characterize tissue and sex related differences in chlamydial infection

Arrested and Aberrant: Effects of Amoxicillin in a Murine Model of Chlamydial Infection

Chlamydia trachomatis is the most common sexually transmitted bacterial disease agent worldwide, and, though frequently asymptomatic, can cause extreme pathology including infertility. Chlamydial species exhibit a unique biphasic developmental cycle. Once attached to a cell surface, infectious elementary bodies (EB) are internalized within an inclusion, the membrane-bound structure in which EB transform to noninfectious, replicable reticulate bodies (RB). After multiple rounds of division, RB condense to form EB, which are released and can infect new host cells. In culture, exposure to stressors, such as beta-lactam antibiotics, induce chlamydiae to reversibly detour from normal development into a noninfectious, viable state termed persistence. Cell culture data suggest that persistent forms are resistant to azithromycin (AZM), a front-line antibiotic, and are able to alter the host transcriptome. Though persistence has been described in culture for over 50 years, whether or not it: i) occurs in vivo; and ii) influences chlamydial pathogenesis, transmission and therapy has remained unresolved. To address these questions, we developed an animal model of persistent chlamydial infection using amoxicillin (AMX) treatment. AMX exposure decreased shedding of infectious chlamydiae in C. muridarum-infected mice without affecting chlamydial viability, demonstrating the presence of persistent chlamydiae. Shedding of infectious EB resumed following AMX cessation. Shedding data and microarray analyses suggested that host immunity might limit chlamydia’s exit from persistence in our model. Thus, we hypothesized that cyclophosphamide (CTX) treatment would increase the magnitude of chlamydial shedding observed after AMX-treatment cessation. CTX treatment increased post-AMX shedding by more than 10-fold compared to AMX-only controls. To determine whether persistent chlamydiae are resistant to antibiotic eradication in vivo, we induced persistence by administering AMX and treated mice with various AZM dosing regimes. Persistently infected mice demonstrated increased treatment failure following AZM therapy compared to productively infected controls. These data suggest that persistent chlamydiae are refractory to treatment in vivo and provide an explanation for the observation that treatment fails in some patients. In addition to creating the first fully characterized, experimentally tractable, in vivo model of chlamydial persistence, these experiments provide evidence that persistent/stressed chlamydial forms may serve as a long-term reservoir of infectious organisms in vivo

Host Nectin-1 Promotes Chlamydial Infection in the Female Mouse Genital Tract, but Is Not Required for Infection in a Novel Male Murine Rectal Infection Model.

Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen, but more than 70% of patients fail to seek treatment due to the asymptomatic nature of these infections. Women suffer from numerous complications from chronic chlamydial infections, which include pelvic inflammatory disease and infertility. We previously demonstrated in culture that host cell nectin-1 knockdown significantly reduced chlamydial titers and inclusion size. Here, we sought to determine whether nectin-1 was required for chlamydial development in vivo by intravaginally infecting nectin-1-/- mice with Chlamydia muridarum and monitoring chlamydial shedding by chlamydial titer assay. We observed a significant reduction in chlamydial shedding in female nectin-1-/- mice compared to nectin-1+/+ control mice, an observation that was confirmed by PCR. Immunohistochemical staining in mouse cervical tissue confirmed that there are fewer chlamydial inclusions in Chlamydia-infected nectin-1-/- mice. Notably, anorectal chlamydial infections are becoming a substantial health burden, though little is known regarding the pathogenesis of these infections. We therefore established a novel male murine model of rectal chlamydial infection, which we used to determine whether nectin-1 is required for anorectal chlamydial infection in male mice. In contrast to the data from vaginal infection, no difference in rectal chlamydial shedding was observed when male nectin-1+/+ and nectin-1-/- mice were compared. Through the use of these two models, we have demonstrated that nectin-1 promotes chlamydial infection in the female genital tract but does not appear to contribute to rectal infection in male mice. These models could be used to further characterize tissue and sex related differences in chlamydial infection

Aberrant Fecal Flora Observed in Guinea Pigs With Pressure Overload Is Mitigated in Animals Receiving Vagus Nerve Stimulation Therapy

Altered gut microbial diversity has been associated with several chronic disease states, including heart failure. Stimulation of the vagus nerve, which innervates the heart and abdominal organs, is proving to be an effective therapeutic in heart failure. We hypothesized that cervical vagus nerve stimulation (VNS) could alter fecal flora and prevent aberrations observed in fecal samples from heart failure animals. To determine whether microbial abundances were altered by pressure overload (PO), leading to heart failure and VNS therapy, a VNS pulse generator was implanted with a stimulus lead on either the left or right vagus nerve before creation of PO by aortic constriction. Animals received intermittent, open-loop stimulation or sham treatment, and their heart function was monitored by echocardiography. Left ventricular end-systolic and diastolic volumes, as well as cardiac output, were impaired in PO animals compared with baseline. VNS mitigated these effects. Metagenetic analysis was then performed using 16S rRNA sequencing to identify bacterial genera present in fecal samples. The abundance of 10 genera was significantly altered by PO, 8 of which were mitigated in animals receiving either left- or right-sided VNS. Metatranscriptomics analyses indicate that the abundance of genera that express genes associated with ATP-binding cassette transport and amino sugar/nitrogen metabolism was significantly changed following PO. These gut flora changes were not observed in PO animals subjected to VNS. These data suggest that VNS prevents aberrant gut flora following PO, which could contribute to its beneficial effects in heart failure patients

Nectin-1 is not required for male mouse rectal chlamydial infection.

<p>Male mice were rectally infected with 1x10⁶ IFU <i>C</i>. <i>muridarum</i> as described in the methods. A) Swab samples every 3 days from day 3 to 24 pi were used in chlamydial titer assays to determine chlamydial shedding. N = 16 and n = 18 for nectin-1^+/+ and nectin-1^-/- groups, respectively. Shedding is reported as the average IFU/mouse +/- SEM at each day pi. Combined data from two independent experiments are shown. B) Immunohistochemical staining of male wild type colon tissue at 430x (left panels) and 630x (right panels). Yellow arrows indicate chlamydial inclusions. Colon tissue was harvested from <i>C</i>. <i>muridarum</i> rectally infected mice at day 24 pi; n = 3. Two representative inclusions are depicted from one wild type mouse. Data represent a single experiment.</p

Nectin-1^-/- female mice have fewer detectable chlamydiae in the lower genital tract.

<p>A) Day 3 pi PCR semi-quantification of chlamydial genomes using 16s DNA normalized to host β-actin. Each group n = 4 and data are representative of 2 independent experiments. B) Day 3 pi PCR semi-quantification of chlamydial viability determined by amplification of chlamydial 16s rRNA normalized to chlamydial 16s DNA and host β-actin. A single data set was analyzed and n = 4 per group. Panels A and B are reported as average integrated intensity +/-SEM. Differences between groups were determined with the unpaired Student’s t-test with p<0.05 considered significant, as indicated by an asterisk (*). Non-significant comparisons are designated NS. C) Representative gel electrophoresis of chlamydial 16s DNA, chlamydial pre-16s RNA, and host β-actin PCR bands from one nectin-1^+/+ and one nectin-1^-/- female mouse.</p

Host nectin-1 is required for chlamydial shedding in intravaginally infected mice.

<p>A) Example of genotypic characterization of nectin-1 heterozygous mice (lanes 1 and 2), nectin-1^+/+ mice (lanes 3 and 4), and nectin-1^-/- mice (lanes 5 and 6). Molecular size ladders are represented by lanes labeled “L”. Nectin-1^+/+ mice exhibit a single band at 639bp, nectin-1^-/- mice exhibit a single band at 459bp and heterozygotes exhibit bands at 639bp and 459bp. B and C) Mice were infected with either 1 x 10³ IFU (B) or 1 x 10⁶ IFU <i>C</i>. <i>muridarum</i> (C) on day 0. Swab samples from days 3 through 21 pi were used in chlamydial titer assays to determine chlamydial shedding. For panel B, n = 10 for the nectin-1^+/+ group and n = 13 for the nectin-1^-/- group. For panel C, n = 19 per group. Shedding data are reported as the average IFU/mouse +/- SEM at each day pi. Shedding data are depicted as the combined data from 2 independent experiments each. Differences in shedding between groups at each day post shedding were determined with the unpaired Student’s t-test with p<0.05 considered significant, as indicated by an asterisk (*).</p

Nectin-1^-/- female mice have fewer detectable chlamydial inclusions in cervical tissue.

<p>A) Immunohistochemical staining of nectin-1^+/+ (upper panels) and nectin-1^-/- (lower panels) <i>C</i>. <i>muridarum</i> infected cervical tissue harvested day 6 pi; n = 5 for each group. Yellow arrows indicate chlamydial inclusions. All mouse cervical samples were stained and cervical samples shown are from two individual mice per experimental group. Data are representative of two independent experiments.</p

Vagus Nerve Stimulation Mitigates Cardiac Symptoms and Alters Inflammatory Markers in Heart Failure Rats

Chronic heart failure (HF) is estimated to affect 23 million people worldwide, and many patients show minimal improvement after treatment with high-potency medications. HF with reduced left ventricular ejection fraction makes up approximately half of cases and is associated with high mortality: a 5-year survival rate of only 25% after hospitalization. This disease is marked by autonomic and cardiac dysfunction, as well as increased inflammatory markers both in the brain and microbiota of the gastrointestinal tract. As a main component of the autonomic nervous system, the vagus nerve has been identified as a potential treatment target for HF. Vagus nerve stimulation (VNS) is thought to help re-balance the autonomic system and has shown promising results in clinical trials for treatment of HF. Although the mechanism of action for VNS remains partially understood, anti-inflammatory pathways have been shown to play a significant role, and these pathways may be enhanced by microbiota signaling via the vagus nerve. The goal of the current study is to provide insight into VNS treatment for HF with reduced ejection fraction via a pressure overload (PO) model. Male Sprague-Dawley rats were randomly divided into age-matched control (n=7), PO (n=6), and PO+VNS (n=11). PO rats underwent aortic constriction (~40%) to induce HF, and a subset of these had VNS leads implanted around the left cervical vagus nerve. Treatment was initiated for PO+VNS rats after reaching a 20% drop in left ventricular relative ejection fraction (EF, p\u3c0.001). VNS was delivered using 1.0 mA pulses at 20 Hz, with 14 sec on-time followed by 66 sec off-time for 2 months to model settings used in successful clinical studies. Echocardiography to image the heart and fecal samples to assess microbiota were collected at regular intervals for all rats. Hearts were weighed at termination for a final heart to body weight ratio, and brains were processed to assess neuroinflammation. Findings indicate that while PO reduced EF ~40% at termination (p\u3c0.05), VNS treatment restored EF back to control levels (p\u3c0.0001 compared to study midpoint). Further, the heart/body weight ratio was increased for PO rats (p\u3c0.05) compared to controls and PO+VNS rats. These data demonstrate that physiological markers of heart failure can be mitigated using these VNS settings. Notably, 66% of microbiota populations altered by PO were prevented with VNS treatment. Further, prolonged VNS significantly affected microbiota populations involved in inflammatory processes. Neuroinflammation was assessed in two key autonomic nuclei: paraventricular nucleus of the hypothalamus and locus coeruleus. PO displayed increased neuroinflammation as measured by microglial density in both regions, and VNS attenuated this effect (p\u3c0.001). These findings indicate relevant contributions of inflammatory mechanisms and microbiome alterations for beneficial VNS effects leading to improved cardiac function in HF