The influence of uterine abnormalities on uterine peristalsis in the non-pregnant uterus:A systematic review

Uterine peristalsis is the rhythmic wave-like motion of the subendometrial layer of the uterus. These contractions change throughout the menstrual cycle in terms of direction, frequency and amplitude, and can be analysed with various methods. Not much is known about uterine peristalsis in patients with uterine abnormalities. To that end, we decided to systematically review the available studies for evidence on the influence of uterine abnormalities, including leiomyomas, endometriosis, adenomyosis and congenital uterine anomalies, on uterine peristalsis. After a systematic search of relevant databases, sixteen eligible studies were included in this review; eight case-control studies and eight controlled prospective cohort studies. The sample sizes ranged from twelve to 205 participants. Various methods of analysing uterine contractions were used, including transvaginal ultrasound, hysterosalpingo-radionuclide scintigraphy, cine MRI and intrauterine pressure measurement. Studies varied in their design, uterine contraction measurement method and patient groups. Generally however, uterine abnormalities do seem to have an influence on uterine peristalsis. Compared to healthy controls, the specific phase of the menstrual cycle (namely the periovulatory and luteal phases) seems to play a major role in the observed effect on uterine contractions. The included studies were difficult to compare directly due to heterogeneity however, and sample sizes were relatively small. Despite these limitations, it can be concluded that uterine abnormalities likely have a menstrual phase-dependent effect on uterine peristalsis and contraction features. These aberrant contractions potentially play a role in the relationship between (benign) uterine abnormalities and infertility, along with other associated symptoms (i.e., dysmenorrhea, abnormal uterine bleeding). It is not yet possible to make a definite conclusion on the nature of this effect however. Further research is needed on objective measurement tools, treatment and clinical consequences of abnormal uterine peristalsis in patients with uterine abnormalities

Diffusiophoresis of latex driven by anionic nanoparticles and their counterions

Hypothesis Diffusiophoresis of colloidal latex particles has been reported for molecular anions and cations of comparable size. In the present study, this phenomenon is observed for two types of charged colloids acting as multivalent electrolyte: (i) anionic charge-stabilised silica nanoparticles or (ii) minimally-charged sterically-stabilised diblock copolymer nanoparticles. Experiments Using a Hele-Shaw cell, a thin layer of relatively large latex particles is established within a sharp concentration gradient of nanoparticles by sequential filling with water, latex particles and nanoparticles. Asymmetric diffusion is observed, which provides strong evidence for diffusiophoresis. Quantification involves turbidity measurements from backlit images. Findings The latex particles diffuse across a concentration gradient of charged nanoparticles and the latex concentration front scales approximately with time1/2. Moreover, the latex particle flux is inversely proportional to the concentration of background salt, confirming electrostatically-driven motion. These observations are consistent with theory recently developed to account for diffusiophoretic motion driven by multivalent ions

Numerical Solutions of ideal two-fluid equations very closed to the event horizon of Schwarzschild black hole

The 3+1 formalism of Thorne, Price and Macdonald has been used to derive the linear two-fluid equations describing transverse and longitudinal waves propagating in the two-fluid ideal collisionless plasmas surrounding a Schwarzschild black hole. The plasma is assumed to be falling in radial direction toward the event horizon. The relativistic two-fluid equations have been reformulate, in analogy with the special relativistic formulation as explained in an earlier paper, to take account of relativistic effects due to the event horizon. Here a WKB approximation is used to derive the local dispersion relation for these waves and solved numerically for the wave number k.Comment: 16 pages, 15 figures. arXiv admin note: text overlap with arXiv:0902.3766, arXiv:0807.459

Genetic and Phenotypic Features of Schizophrenia in the UK Biobank

IMPORTANCE Large-scale biobanks provide important opportunities for mental health research, but selection biases raise questions regarding the comparability of individuals with those in clinical research settings. OBJECTIVE To compare the genetic liability to psychiatric disorders in individuals with schizophrenia in the UK Biobank with individuals in the Psychiatric Genomics Consortium (PGC) and to compare genetic liability and phenotypic features with participants recruited from clinical settings. DESIGN, SETTING, AND PARTICIPANTS This cross-sectional study included participants from the population-based UK Biobank and schizophrenia samples recruited from clinical settings (CLOZUK, CardiffCOGS, Cardiff F-Series, and Cardiff Affected Sib-Pairs). Data were collected between January 1993 and July 2021. Data analysis was conducted between July 2021 and June 2023. MAIN OUTCOMES AND MEASURES A genome-wide association study of UK Biobank schizophrenia case-control status was conducted, and the results were compared with those from the PGC via genetic correlations. To test for differences with the clinical samples, polygenic risk scores (PRS) were calculated for schizophrenia, bipolar disorder, depression, and intelligence using PRS-CS. PRS and phenotypic comparisons were conducted using pairwise logistic regressions. The proportions of individuals with copy number variants associated with schizophrenia were compared using Firth logistic regression. RESULTS The sample of 517 375 participants included 1438 UK Biobank participants with schizophrenia (550 [38.2%] female; mean [SD] age, 54.7 [8.3] years), 499 475 UK Biobank controls (271 884 [54.4%] female; mean [SD] age, 56.5 [8.1] years), and 4 schizophrenia research samples (4758 [28.9%] female; mean [SD] age, 38.2 [21.0] years). Liability to schizophrenia in UK Biobank was highly correlated with the latest genome-wide association study from the PGC (genetic correlation, 0.98; SE, 0.18) and showed the expected patterns of correlations with other psychiatric disorders. The schizophrenia PRS explained 6.8% of the variance in liability for schizophrenia case status in UK Biobank. UK Biobank participants with schizophrenia had significantly lower schizophrenia PRS than 3 of the clinically ascertained samples and significantly lower rates of schizophrenia-associated copy number variants than the CLOZUK sample. UK Biobank participants with schizophrenia had higher educational attainment and employment rates than the clinically ascertained schizophrenia samples, lower rates of smoking, and a later age of onset of psychosis. CONCLUSIONS AND RELEVANCE Individuals with schizophrenia in the UK Biobank, and likely other volunteer-based biobanks, represent those less severely affected. Their inclusion in wider studies should enhance the representation of the full spectrum of illness severity

Investigating differences in population recovery rates of two sympatrically nesting sea turtle species

This is the final version. Available on open access from Wiley via the DOI in this recordData Availability Statement: Data are available from corresponding author upon reasonable request.Estimating life‐history traits and understanding their variation underpins the management of long‐lived, migratory animals, while knowledge of recovery dynamics can inform the management of conservation‐dependent species. Using a combination of nest counts and individual‐based life‐history data collected since 1993, we explore the drivers underlying contrasting population recovery rates of sympatrically nesting loggerhead (Caretta caretta) and green (Chelonia mydas) turtles in North Cyprus. We found that nest counts of loggerhead and green turtles from 28 beaches across the island increased by 46% and 162%, respectively over the past 27 years. A Bayesian state‐space model revealed that, at our individual‐based monitoring site, nesting of green turtles increased annually at four times the rate of that of loggerhead turtles. Furthermore, we found that loggerhead turtles nesting at the individual‐based monitoring site had stable reproductive parameters and average adult survival for the species and are the smallest breeding adults globally. Based on results from multiple matrix model scenarios, we propose that higher mortality rates of individuals in all age classes (likely driven by differences in life history and interaction with fisheries), rather than low reproductive output, are impeding the recovery of this species. While the increase in green turtles is encouraging, the Mediterranean population is estimated to have around 3,400 adults and is restricted to the Eastern Basin. The recovery of loggerhead turtles is likely to be compromised until mortality rates in the region are adequately quantified and mitigated. As survival of immature individuals is a powerful driver for sea turtle population numbers, additional efforts should target management at pelagic and neritic foraging areas. Understanding threats faced by immature life stages is crucial to accurately parameterise population models and to target conservation actions for long‐lived marine vertebrates

Counting the bodies: Estimating the numbers and spatial variation of Australian reptiles, birds and mammals killed by two invasive mesopredators

Aim Introduced predators negatively impact biodiversity globally, with insular fauna often most severely affected. Here, we assess spatial variation in the number of terrestrial vertebrates (excluding amphibians) killed by two mammalian mesopredators introduced to Australia, the red fox (Vulpes vulpes) and feral cat (Felis catus). We aim to identify prey groups that suffer especially high rates of predation, and regions where losses to foxes and/or cats are most substantial. Location Australia. Methods We draw information on the spatial variation in tallies of reptiles, birds and mammals killed by cats in Australia from published studies. We derive tallies for fox predation by (i) modelling continental-scale spatial variation in fox density, (ii) modelling spatial variation in the frequency of occurrence of prey groups in fox diet, (iii) analysing the number of prey individuals within dietary samples and (iv) discounting animals taken as carrion. We derive point estimates of the numbers of individuals killed annually by foxes and by cats and map spatial variation in these tallies. Results Foxes kill more reptiles, birds and mammals (peaking at 1071 km−2 year−1) than cats (55 km−2 year−1) across most of the unmodified temperate and forested areas of mainland Australia, reflecting the generally higher density of foxes than cats in these environments. However, across most of the continent – mainly the arid central and tropical northern regions (and on most Australian islands) – cats kill more animals than foxes. We estimate that foxes and cats together kill 697 million reptiles annually in Australia, 510 million birds and 1435 million mammals. Main conclusions This continental-scale analysis demonstrates that predation by two introduced species takes a substantial and ongoing toll on Australian reptiles, birds and mammals. Continuing population declines and potential extinctions of some of these species threatens to further compound Australia's poor contemporary conservation record

Characterization of Antibodies against Receptor Activity-Modifying Protein 1 (RAMP1): A Cautionary Tale

Calcitonin gene-related peptide (CGRP) is a key component of migraine pathophysiology, yielding effective migraine therapeutics. CGRP receptors contain a core accessory protein subunit: receptor activity-modifying protein 1 (RAMP1). Understanding of RAMP1 expression is incomplete, partly due to the challenges in identifying specific and validated antibody tools. We profiled antibodies for immunodetection of RAMP1 using Western blotting, immunocytochemistry and immunohistochemistry, including using RAMP1 knockout mouse tissue. Most antibodies could detect RAMP1 in Western blotting and immunocytochemistry using transfected cells. Two antibodies (844, ab256575) could detect a RAMP1-like band in Western blots of rodent brain but not RAMP1 knockout mice. However, cross-reactivity with other proteins was evident for all antibodies. This cross-reactivity prevented clear conclusions about RAMP1 anatomical localization, as each antibody detected a distinct pattern of immunoreactivity in rodent brain. We cannot confidently attribute immunoreactivity produced by RAMP1 antibodies (including 844) to the presence of RAMP1 protein in immunohistochemical applications in brain tissue. RAMP1 expression in brain and other tissues therefore needs to be revisited using RAMP1 antibodies that have been comprehensively validated using multiple strategies to establish multiple lines of convincing evidence. As RAMP1 is important for other GPCR/ligand pairings, our results have broader significance beyond the CGRP field

Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants

Coordinated programs of gene expression drive brain development. It is unclear which transcriptional programs, in which cell-types, are affected in neuropsychiatric disorders such as schizophrenia. Here we integrate human genetics with transcriptomic data from differentiation of human embryonic stem cells into cortical excitatory neurons. We identify transcriptional programs expressed during early neurogenesis in vitro and in human foetal cortex that are down-regulated in DLG2−/− lines. Down-regulation impacted neuronal differentiation and maturation, impairing migration, morphology and action potential generation. Genetic variation in these programs is associated with neuropsychiatric disorders and cognitive function, with associated variants predominantly concentrated in loss-of-function intolerant genes. Neurogenic programs also overlap schizophrenia GWAS enrichment previously identified in mature excitatory neurons, suggesting that pathways active during prenatal cortical development may also be associated with mature neuronal dysfunction. Our data from human embryonic stem cells, when combined with analysis of available foetal cortical gene expression data, de novo rare variants and GWAS statistics for neuropsychiatric disorders and cognition, reveal a convergence on transcriptional programs regulating excitatory cortical neurogenesis

Search for Gravitational Waves Associated with Gamma-Ray Bursts Detected by Fermi and Swift during the LIGO-Virgo Run O3b

We search for gravitational-wave signals associated with gamma-ray bursts (GRBs) detected by the Fermi and Swift satellites during the second half of the third observing run of Advanced LIGO and Advanced Virgo (2019 November 1 15:00 UTC-2020 March 27 17:00 UTC). We conduct two independent searches: A generic gravitational-wave transients search to analyze 86 GRBs and an analysis to target binary mergers with at least one neutron star as short GRB progenitors for 17 events. We find no significant evidence for gravitational-wave signals associated with any of these GRBs. A weighted binomial test of the combined results finds no evidence for subthreshold gravitational-wave signals associated with this GRB ensemble either. We use several source types and signal morphologies during the searches, resulting in lower bounds on the estimated distance to each GRB. Finally, we constrain the population of low-luminosity short GRBs using results from the first to the third observing runs of Advanced LIGO and Advanced Virgo. The resulting population is in accordance with the local binary neutron star merger rate. © 2022. The Author(s). Published by the American Astronomical Society