Nitrogen transfer between clover and wheat in an intercropping experiment

A novel approach to the problem of improving nitrogen supply in organic farming is to use intercropping of cereals with a legume to provide nitrogen transfer within a season and/or to following crops. The affects of intercropping were studied in a column experiment using mixtures of winter wheat (Triticum aestivum cv. Claire), with white clover (w.c.) (Trifolium repens cv. Barblanca) and with red clover (r.c.) (Trifolium pratense cv. Britta). The effects of cutting and removal above ground clover material with and without additional soil disturbance were compared to leaving clover plants in situ and intercropped with wheat in a split root design. Wheat and clover plants, as monocultures, were used for the controls. 15N ammonium nitrate solution was applied. The wheat seeds were sown into the column without nitrogen. We found that the cutting treatment produced the highest yield of wheat. Available ammonium-N in the soil was greatest in the clover control treatment for the column with only red clover roots and in the cutting+soil disturbance treatment for the column with only white clover roots. Available nitrate-N was greatest in the soil disturbance treatment in the column with clover and wheat roots for both red and white clover. The cutting treatment produced the highest yield of wheat

Steep Slopes and Preferred Breaks in GRB Spectra: the Role of Photospheres and Comptonization

The role of a photospheric component and of pair breakdown is examined in the internal shock model of gamma-ray bursts. We discuss some of the mechanisms by which they would produce anomalously steep low energy slopes, X-ray excesses and preferred energy breaks. Sub-relativistic comptonization should dominate in high comoving luminosity bursts with high baryon load, while synchrotron radiation dominates the power law component in bursts which have lower comoving luminosity or have moderate to low baryon loads. A photosphere leading to steep low energy spectral slopes should be prominent in the lowest baryon loadComment: ApJ'00, in press; minor revs. 10/5/99; (uses aaspp4.sty), 15 pages, 3 figure

Improving supply and phosphorous use efficiency in organic farming systems

Phosphorus (P) is an essential plant nutrient that needs to be managed carefully in organic systems so that crop yield and quality remain sustainable without contributing to environmental damage, particularly that associated with eutrophication. Under organic regulations, minimally processed rock phosphate (PR) can be used to amend low P fertility soils, although the solubility is extremely low at optimum soil pH for most crop growth (pH 6.5). This paper describes a project (PLINK) which aims to develop methods of improving P efficiency on organic farms, although the same approaches may also be applicable on conventional and low-input farms. The methodologies that the project is developing include the fermentation and composting of crop waste material with PR in order to solubilise P and make it more available to the crop. Some initial results are described here. In addition, the project will investigate the alteration of the rotation to include crops or varieties with high P uptake efficiency, or roots that possess acidifying properties which improve P availability for following crops

Indicator systems - resource use in organic systems

A balanced use of resources within organic farming systems is required to maintain sustainable systems. Hence, it is essential to have tools that can assess the use of resources within the farming system and their impact on the environment. The range of tools that have been developed include those assessing local farm-scale issues together with those that assess impacts at the global scale. At the global scale assessments are usually made on the basis of a unit of product whereas at the local scale assessments can also be made on an area basis. In addition, the tools also assess a variety of issues, e.g. biodiversity, pollution potential, energy and water use. The level of detail required for the different assessment tools differs substantially; nevertheless it is essential that the indicator systems developed are based on sound knowledge, are acceptable to the farmers and can guide their future actions

Specific Enhancement of Transplantation Immunity with Heat-killed Mycobacterium Butyricum and Immunizing Extracts from Adenovirus 12-Induced Tumour Cells

Transplant immunity to adenovirus 12-induced tumour cells was demonstrated in CBA mice which had been previously immunized with extracts of homologous tumour cells. Immunization of mice with tumour cell extract together with heat-killed Mycobacterium butyricum gave better transplant immunity to tumour cell challenge than tumour extracts alone. Mice immunized with Mycobacterium butyricum alone prior to challenge with tumour cells, did not show any significant difference in the incidence of tumours from control mice

Bi-Directional Relativistic Jets of the Radio Galaxy 1946+708: Constraints on the Hubble Constant

We present measurements of bi-directional motions in the jets of the radio galaxy 1946+708 at z=0.101. This is a Compact Symmetric Object with striking S-symmetry. Sensitive 15 GHz observations reveal a compact component at the center of symmetry with a strongly inverted spectrum, that we identify as the core. From five 4.9 GHz observations spread over 4 years we have determined the velocities of four compact jet components. If simple kinematic models can be applied then the inclination of the source and the bulk jet velocity can be directly determined for any assumed value of the Hubble constant. Conversely, the measurements already place constraints on the Hubble constant, and we show how further observations of 1946+708 can yield an increasingly accurate determination of H_0.Comment: in press at ApJ Letters, 12 page LaTex document includes 5 postscript figure

Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site

The quinol-fumarate reductase (QFR) respiratory complex of Escherichia coli is a four-subunit integral-membrane complex that catalyzes the final step of anaerobic respiration when fumarate is the terminal electron acceptor. The membrane-soluble redox-active molecule menaquinol (MQH(2)) transfers electrons to QFR by binding directly to the membrane-spanning region. The crystal structure of QFR contains two quinone species, presumably MQH(2), bound to the transmembrane-spanning region. The binding sites for the two quinone molecules are termed Q(P) and Q(D), indicating their positions proximal Q(P)) or distal (Q(D)) to the site of fumarate reduction in the hydrophilic flavoprotein and iron-sulfur protein subunits. It has not been established whether both of these sites are mechanistically significant. Co-crystallization studies of the E. coli QFR with the known quinol-binding site inhibitors 2-heptyl-4-hydroxyquinoline-N-oxide and 2-[1-(p-chlorophenyl)ethyl] 4,6-dinitrophenol establish that both inhibitors block the binding of MQH(2) at the Q(P) site. In the structures with the inhibitor bound at Q(P), no density is observed at Q(D), which suggests that the occupancy of this site can vary and argues against a structurally obligatory role for quinol binding to Q(D). A comparison of the Q(P) site of the E. coli enzyme with quinone-binding sites in other respiratory enzymes shows that an acidic residue is structurally conserved. This acidic residue, Glu-C29, in the E. coli enzyme may act as a proton shuttle from the quinol during enzyme turnover