11 research outputs found
Identification of glucocorticoid-induced leucine zipper as a key regulator of tumor cell proliferation in epithelial ovarian cancer
<p>Abstract</p> <p>Background</p> <p>Little is known about the molecules that contribute to tumor progression of epithelial ovarian cancer (EOC), currently a leading cause of mortality from gynecological malignancies. Glucocorticoid-Induced Leucine Zipper (GILZ), an intracellular protein widely expressed in immune tissues, has been reported in epithelial tissues and controls some of key signaling pathways involved in tumorigenesis. However, there has been no report on GILZ in EOC up to now. The objectives of the current study were to examine the expression of GILZ in EOC and its effect on tumor cell proliferation.</p> <p>Results</p> <p>GILZ expression was measured by immunohistochemical staining in tissue sections from 3 normal ovaries, 7 benign EOC and 50 invasive EOC. GILZ was not detected on the surface epithelium of normal ovaries and benign tumors. In contrast, it was expressed in the cytoplasm of tumor cells in 80% EOC specimens. GILZ immunostaining scores correlated positively to the proliferation marker Ki-67 (Spearman test in univariate analysis, <it>P </it>< 0.00001, r = 0.56). They were also higher in tumor cells containing large amounts of phosphorylated protein kinase B (p-AKT) (unpaired t test, <it>P </it>< 0.0001). To assess the effect of GILZ on proliferation and AKT activation, we used the BG-1 cell line derived from ovarian tumor cells as a cellular model. GILZ expression was either enhanced by stable transfection or decreased by the use of small interfering (si) RNA targeting GILZ. We found that GILZ increased cell proliferation, phospho-AKT cellular content and AKT kinase activity. Further, GILZ upregulated cyclin D1 and phosphorylated retinoblastoma (p-Rb), downregulated cyclin-dependent kinase inhibitor p21, and promoted the entry into S phase of cell cycle.</p> <p>Conclusion</p> <p>The present study is the first to identify GILZ as a molecule produced by ovarian cancer cells that promotes cell cycle progression and proliferation. Our findings clearly indicate that GILZ activates AKT, a crucial signaling molecule in tumorigenesis. GILZ thus appears as a potential key molecule in EOC.</p
NY-ESO-1-Specific Circulating CD4+ T Cells in Ovarian Cancer Patients Are Prevalently TH1 Type Cells Undetectable in the CD25+FOXP3+Treg Compartment
Spontaneous CD4+ T-cell responses to the tumor-specific antigen NY-ESO-1 (ESO) are frequently found in patients with epithelial ovarian cancer (EOC). If these responses are of effector or/and Treg type, however, has remained unclear. Here, we have used functional approaches together with recently developed MHC class II/ESO tetramers to assess the frequency, phenotype and function of ESO-specific cells in circulating lymphocytes from EOC patients. We found that circulating ESO-specific CD4+ T cells in EOC patients with spontaneous immune responses to the antigen are prevalently TH1 type cells secreting IFN-Îł but no IL-17 or IL-10 and are not suppressive. We detected tetramer+ cells ex vivo, at an average frequency of 1â¶25000 memory cells, that is, significantly lower than in patients immunized with an ESO vaccine. ESO tetramer+ cells were mostly effector memory cells at advanced stages of differentiation and were not detected in circulating CD25+FOXP3+Treg. Thus, spontaneous CD4+ T-cell responses to ESO in cancer patients are prevalently of TH1 type and not Treg. Their relatively low frequency and advanced differentiation stage, however, may limit their efficacy, that may be boosted by immunogenic ESO vaccines
RÎle du Glucocorticoid-induced Leucine Zipper (GILZ) dans le cancer épithélial de l'ovaire
LE KREMLIN-B.- PARIS 11-BU MĂ©d (940432101) / SudocSudocFranceF
<i>Ex vivo</i> assessment of ESO-specific CD4<sup>+</sup> T cells using DR52b/ESO tetramers.
<p>CD4<sup>+</sup> T cells from DR52b<sup>+</sup> healthy donors (HD) and patients were stained <i>ex vivo</i> with DR52b/ESO<sub>119â143</sub> tetramers and mAb specific for CD45RA, CD25, CD127, CCR7 and CD27 and analyzed by flow cytometry. <b>A</b>. Dot plots for one HD and one EOC patient are shown. Numbers in dot plots correspond to the percentage of tetramer<sup>+</sup> cells among CD45RA<sup>â</sup> memory cells. Data for all EOC patients with spontaneous immune responses to ESO (S) are shown in comparison to the frequency (mean ± SD) of ESO tetramer<sup>+</sup> cells in post-vaccine samples from patients having received a recombinant ESO vaccine (V) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022845#pone.0022845-Ayyoub2" target="_blank">[17]</a>. <b>B</b>. Dot plots show the expression of CD25 and CD127 in total memory cells and in tetramer<sup>+</sup> cells of one EOC patient. Data corresponding to the proportion of conventional, CD25<sup>â</sup>CD127<sup>+</sup> and CD25<sup>â</sup>CD127<sup>â</sup>, and Treg, CD25<sup>+</sup>CD127<sup>â</sup>, populations within tetramer<sup>+</sup> cells for all EOC patients (S) are summarized and compared to the proportion (mean ± SD) of these populations in vaccine-induced tetramer<sup>+</sup> cells (V). <b>C</b>. Dot plots show the expression of CCR7 and CD27 in total memory cells and in tetramer<sup>+</sup> cells of one EOC patient. Data corresponding to the proportion of CCR7<sup>+</sup>, CCR7<sup>â</sup>CD27<sup>+</sup> and CCR7<sup>â</sup>CD27<sup>â</sup> populations within tetramer<sup>+</sup> cells for all EOC patients (S) are summarized and compared to the proportion (mean ± SD) of these populations in vaccine-induced tetramer<sup>+</sup> cells (V). Statistical analyses were performed using a standard two-tailed <i>t</i>-test.</p
Functional assessment of memory conventional and regulatory CD4<sup>+</sup> T-cell subsets in circulating lymphocytes of healthy donors and EOC patients.
<p><i>Ex vivo</i>-sorted memory conventional, CD25<sup>â</sup>CD127<sup>+</sup> and CD25<sup>â</sup>CD127<sup>â</sup>, and Treg, CD25<sup>+</sup>CD127<sup>â</sup>, populations from healthy donors (HD, nâ=â12) and patients (P, nâ=â12) were stimulated <i>in vitro</i> and day 12 cultures were assessed for IFN-Îł, IL-10 and IL-17 production, following stimulation with PMA and ionomycin, in a 4-h intracellular cytokine secretion assay and analyzed by flow cytometry. Dot plots for one donor are shown in <b>A</b> and data for all healthy donors and patients are summarized in <b>B</b>. Statistical analyses were performed using a standard two-tailed <i>t</i>-test.</p
Phenotypic assessment of memory conventional and regulatory CD4<sup>+</sup> T-cell subsets in circulating lymphocytes of healthy donors and EOC patients.
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodies and analyzed by flow cytometry. Expression of CD25 and CD127 defines 3 populations of memory (CD45RA<sup>â</sup>) CD4<sup>+</sup> T cells: conventional CD25<sup>â</sup>CD127<sup>+</sup> and CD25<sup>â</sup>CD127<sup>â</sup> and Treg CD25<sup>+</sup>CD127<sup>â</sup> (left dot plot, numbers correspond to the proportion of each subset among memory CD4<sup>+</sup> T cells). Histograms show the expression of FOXP3 in the defined memory CD4<sup>+</sup> T-cell subsets. <b>B</b>. The proportion of conventional CD25<sup>â</sup>CD127<sup>+</sup> and CD25<sup>â</sup>CD127<sup>â</sup> and Treg CD25<sup>+</sup>CD127<sup>â</sup> subsets, defined in <b>A</b>, among memory CD4<sup>+</sup> T cells of healthy donors (HD, nâ=â27) and patients (P, nâ=â18).</p
Assessment of the suppressive activity of ESO-specific CD4<sup>+</sup> T-cell polyclonal populations.
<p>ESO-specific cells were isolated from peptide-stimulated CD25<sup>â</sup>CD127<sup>+</sup> and CD25<sup>â</sup>CD127<sup>â</sup> CD4<sup>+</sup> T-cell cultures (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0022845#pone-0022845-g004" target="_blank">Figure 4</a>) by tetramer-guided (NA017) or IFN-Îł-guided (NA114) flow cytometry cell sorting and expanded <i>in vitro</i>. The resulting polyclonal cultures contained >80% ESO-specific cells. <b>A</b>. ESO-specific polyclonal cultures as well as polyclonal cultures from the ESO<sup>â</sup> fraction and control cultures of <i>in vitro</i>-expanded conventional memory CD4<sup>+</sup> T cells (M) and memory Treg (MTreg), isolated <i>ex vivo</i> from healthy individuals, were stained with FOXP3-specific mAb and analyzed by flow cytometry. Numbers in dot plots correspond to the mean fluorescence intensity (MFI) of FOXP3 staining. <b>B</b>. The suppressive activity of ESO-specific and control polyclonal populations was assessed by co-culture with CFSE-labeled conventional CD4<sup>+</sup> T cells, at a responder:suppressor ratio of 1â¶1, in the presence of irradiated monocytes and PHA. Dot plots show the CFSE-dilution profile in the absence of test population (left) and in the presence of the indicated test populations. Numbers in histograms correspond the percentage of undivided cells. Results corresponding to the calculated% suppression are shown for all tested populations.</p
Assessment of ESO-specific antibody responses in sera of EOC patients.
<p><b>A</b>. The presence of ESO-specific Ab in patients' sera was assessed by ELISA. Sera were initially assessed at a 1â¶100 dilution on rESO-coated or on control uncoated plates. Sera were scored as ESO Ab<sup>+</sup> if the optical density (OD) values obtained on rESO-coated plates were both at least 3 folds higher than those obtained for the same sample on uncoated plates and higher than the mean+6xSD of OD values obtained with sera from healthy individuals on rESO-coated plates (nâ=â53, mean+6xSD â=â750, data not shown). <b>B</b>. Serial dilutions of ESO Ab<sup>+</sup> sera were tested on rESO-coated plates. Serum titer was calculated as the serum dilution yielding 50% of maximal OD.</p