96 research outputs found
Morphological patterns of the heteropycnotic chromatin and nucleolar material in meiosis and spermiogenesis of some Pentatomidae (Heteroptera)
Meiotic chromosomes and nucleolar behavior in testicular cells of the grassland spittlebugs Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana (Hemiptera, Auchenorrhyncha)
Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a âbunch of grapesâ; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial; metaphases I were circular or linear and anaphase showed late migration of the sex chromosome. The chromosome complement had 2n = 19, except for N. entreriana (2n = 15); the spermatids were round with heteropycnotic material in the center and elongated with conspicuos chromatin. The analysis of testes after silver nitrate staining showed polyploid nuclei with three large and three smaller nucleolar bodies. Early prophase cells had an intensely stained nucleolar body located close to the chromatin and another less evident body located away from the chromatin. The nucleolar bodies disintegrated during diplotene. Silver staining occurred in two autosomes, in terminal and subterminal locations, the latter probably corresponding to the nucleolus organizer regions (NORs). The spermatids were round with a round nucleolar body and silver staining was observed in the medial and posterior region of the elongated part of the spermatid head
Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques
Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band, but at different locations, whereas this band is absent in P. nocens. Heterochromatic bands are neither AT nor GC rich as revealed by fluorescent banding. In situ hybridization with an 18S rDNA probe revealed a signal on chromosome II in a similar location to the C positive band in the three species. The apparent lack of morphologically distinct sex chromosomes is consistent with proposals of environmental sex determination in the genus. Small differences detected in chromosome length and morphology suggests that chromosomes have been highly conserved during the evolutionary radiation of Pseudacteon. Possible mechanisms of karyotype evolution in the three species are suggested
Nodal-Dependent Mesendoderm Specification Requires the Combinatorial Activities of FoxH1 and Eomesodermin
Vertebrate mesendoderm specification requires the Nodal signaling pathway and its transcriptional effector FoxH1. However, loss of FoxH1 in several species does not reliably cause the full range of loss-of-Nodal phenotypes, indicating that Nodal signals through additional transcription factors during early development. We investigated the FoxH1-dependent and -independent roles of Nodal signaling during mesendoderm patterning using a novel recessive zebrafish FoxH1 mutation called midway, which produces a C-terminally truncated FoxH1 protein lacking the Smad-interaction domain but retaining DNAâbinding capability. Using a combination of gel shift assays, Nodal overexpression experiments, and genetic epistasis analyses, we demonstrate that midway more accurately represents a complete loss of FoxH1-dependent Nodal signaling than the existing zebrafish FoxH1 mutant schmalspur. Maternal-zygotic midway mutants lack notochords, in agreement with FoxH1 loss in other organisms, but retain near wild-type expression of markers of endoderm and various nonaxial mesoderm fates, including paraxial and intermediate mesoderm and blood precursors. We found that the activity of the T-box transcription factor Eomesodermin accounts for specification of these tissues in midway embryos. Inhibition of Eomesodermin in midway mutants severely reduces the specification of these tissues and effectively phenocopies the defects seen upon complete loss of Nodal signaling. Our results indicate that the specific combinations of transcription factors available for signal transduction play critical and separable roles in determining Nodal pathway output during mesendoderm patterning. Our findings also offer novel insights into the co-evolution of the Nodal signaling pathway, the notochord specification program, and the chordate branch of the deuterostome family of animals
Cryptic speciation in the Triatoma sordida subcomplex (Hemiptera, Reduviidae) revealed by chromosomal markers
Friction forces position the neural anlage
During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo
Geochemistry, geochronology, and fluid inclusion study of the Late Cretaceous Newton epithermal gold deposit, British Columbia
Newton is an intermediate-sulfidation epithermal gold deposit related to Late Cretaceous continental arc magmatism in south-central British Columbia. Disseminated gold mineralization occurs in quartz-sericite-altered Late Cretaceous felsic volcanic rocks, and feldspar-quartz-hornblende porphyry and quartz-feldspar porphyry intrusions. The mineralization can be divided into 3 stages: (1) disseminated pyrite with microscopic gold inclusions, and sparse quartz-pyrite ± molybdenite veins; (2) disseminated marcasite with microscopic gold inclusions and minor base metal-sulfides; and (3) polymetallic veins of pyrite-chalcopyrite-sphalerite-arsenopyrite.
Re-Os dating of molybdenite from a stage 1 vein yielded an age of 72.1 ± 0.3 Ma (McClenaghan 2013). The age of the host rocks has been constrained by U-Pb dating of zircon: Late Cretaceous felsic volcanic rocks: 72.1 ± 0.6 Ma (Amarc Resources Ltd., unpublished data, reported in McClenaghan 2013); feldspar-quartz-hornblende porphyry: 72.1 ± 0.5 Ma; quartz-feldspar porphyry: 70.9 ± 0.5 Ma (Amarc Resources Ltd., unpublished data, reported in McClenaghan 2013). The mineralized rocks are intruded by a barren diorite, with an age of 69.3 ± 0.4 Ma.
Fluid inclusions in quartzâpyrite ± molybdenite ± gold veins yielded an average homogenization temperature of 313° ± 51°C (n = 82) and salinity of 4.8 ± 0.9 wt.% NaCl equiv. (n = 46), suggesting that a relatively hot and saline fluid likely of magmatic origin was responsible for the first stage of mineralization. Some evidence for boiling was also observed in the veins. However, the bulk of the gold mineralization occurs as disseminations in the wallrocks, suggesting that wallrock reactions were the main control on ore deposition.
Keywords: Newton, Intermediate-sulfidation, Epithermal, Gold, British ColumbiaThe accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Comparative meiotic studies in Triatoma sordida (Stal) and T. guasayana Wygodzinsky & Abalos (Reduviidae, Heteroptera)
Triatoma sordida and T. guasayana are competent Trypanosoma cruzi
vectors, with overlapping distribution areas in Argentina. Both species
are morphologically similar, and their immature stages are hard to
discriminate. Cytogenetic studies in the genus Triatoma reveal scarce
karyotypic variations, being 2n= 20 + XY the most frequent diploid
number in males. In the present work the meiotic behaviour of
different Argentinian populations of T. sordida and T. guasayana has
been analyzed; the meiotic karyotype of both species has also been
compared. The species differ in total chromosome area and in the
relative area of the sex chromosomes. These meiotic karyotypic
differences constitute an additional tool for the taxonomic
characterization of T. sordida and T. guasayana. The analysis of an
interpopulation hybrid of T. sordida (Brazil x Argentina) reveals a
regular meiotic behaviour, despite the presence of heteromorphic
bivalents. Our observations support the hypothesis that karyotype
variations through the gain or loss of heterochromatin can not be
considered as a primary mechanism of reproductive isolation in
Triatoma
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