The use of computer-based assessments in a field biology module

Formative computer-based assessments (CBAs) for self-instruction were introduced into a Year-2 field biology module. These CBAs were provided in ‘tutorial’ mode where each question had context-related diagnostic feedback and tutorial pages, and a self-test mode where the same CBA returned only a score. The summative assessments remained unchanged and consisted of an unseen CBA and written reports of field investigations. When compared with the previous three year-cohorts, the mean score for the summative CBA increased after the introduction of formative CBAs, whereas mean scores for written reports did not change. It is suggested that the increase in summative CBA mean score reflects the effectiveness of the formative CBAs in widening the students’ knowledge base. Evaluation of all assessments using an Assessment Experience Questionnaire indicated that they satisfied the ‘11 conditions under which assessment supports student learning’. Additionally, evidence is presented that the formative CBAs enhanced self-regulated student learning

Learning support for mature, part-time, evening students: providing feedback via frequent, computer-based assessments

A new module in our first year Biology curriculum was used as a vehicle to test strategies for improving learning support. To this end, we have administered frequent CBA, incorporating extensive feedback, both to pace the students’ study efforts and to pinpoint areas in which additional help from lecturers may be required. Three of the 7 CBA provided through the 15-week course were initially given as open-book summative tests, thus contributing to the overall mark for the module. Other CBA were formative: these included repeats of the summative CBA made available for revision purposes, as well as other CBA which focused mainly on aspects of the course that were summatively assessed by other means. A closedbook final exam, also computer-based, was given in the final week as a comprehensive assessment. We have evaluated the utility and effectiveness of our approach by surveying student opinion via questionnaires, examining patterns and extent of student use of formative assessments, and by analysing grades for the summative CBA. We have found the students’ perceptions of the approach to be largely positive and that the formative CBA were well-used, especially as revision aids for the final exam. Our analysis further indicates that the style of the assessments may have been especially helpful to students whose first language is not English

Student perceptions of computer-based formative assessments in a semi-distance module

The evaluation of student views of computer-based assessment is usually based on questionnaires that solicit opinions about this form of assessment in isolation, and often in a context where the computer-based assessment is a novel resource. Being concerned to avoid any possible ‘novelty’ effect on students’ views on the utility of computer-based assessment as a learning resource, we designed our evaluation to concentrate on the assessment experience as a whole

Myth and fact in the origins of cellular life on Earth

Research into the origins of the first protocell is of a multidisciplinary nature. It draws evidence from what we know about the Earth’s early atmosphere and environment, and about the most ancient features of the cell’s structure and composition. Such data provides the input for the hypothesis generation and experimental reconstruction necessary to mimic steps in the formation of the first protocell. While research into the origins of the first protocell is condemned to focus upon laboratory experiments, it should be guided by a detailed study of real evidence pertaining to the environment on Earth 4 billion years ago. In this review, we take stock of the research that has been performed to date across the main disciplines of earth sciences, biochemistry, and molecular biology. We seek to identify the progress made in laying down a sequence for the events that led up to the first protocell. We also assess the strengths and weaknesses of the experimental designs and suggest some future approaches. While the field has made many important advances, from the original Stanley Miller experiment establishing ‘life from chemistry’ products such as amino acids, through to Deamer’s findings on fatty acid membranes and Szostack’s work on lipids, there is still a long and challenging journey ahead to understand how cellular life began. The experiments required to make more rapid progress in the field will likely be more elaborate, costly, and time consuming

Computer-based and computer-assisted tests to assess procedural and conceptual knowledge

It is well known by those at the ‘chalk face’ that learning is not necessarily an outcome of teaching. “Students often fail to spontaneously use what they have learned in a new setting despite the fact that it is highly relevant” (Schwarz et al., 1999) a phenomenon Whitehead referred to as “inert knowledge” (Whitehead, 1929). However, judicious and appropriate use of assessment, as a way of checking for learning rather than to reveal what students don’t know (i.e. for ‘punishment’!) or simply to produce a rank order, can ameliorate this problem by promoting conceptual understanding and encouraging students to employ deep learning approaches

A cogestão no sistema penitenciário do Rio Grande do Norte: limites e contribuições

As discussões acerca da crise que permeia o Sistema Prisional norte rio-grandense só têm aumentado nos últimos anos. Aponta-se a ineficiência do Estado na gestão das prisões em contrapartida a eficiência da iniciativa privada. Associado a isso o Estado brasileiro vem delegando cada vez mais a provisão dos seus serviços à entes privados e na esteira desse novo modelo de gestão da coisa pública é cada vez mais frequente a gerência de serviços prisionais sob o comando de empresas particulares. Os defensores desse modelo de gestão compartilhada buscam fundamentação nos modelos norte-americanos e francês, países esses que investem milhões em sistema carcerário, enquanto o RN pouco injeta recursos que priorizem a (re)integração do interno ao convívio social. Assim como maneira de discutir sobre novas ferramentas para a elaboração de políticas públicas para o sistema prisional do RN, o trabalho de revisão bibliográfica em livros e artigos busca proporcionar reflexões a respeito da contribuição desse novo modelo de provisão de operação de serviços prisionais, bem como seus limites na construção da política carcerária no RN

Atmospheric chemistry regimes in intercontinental air traffic corridors:Ozone versus NOx sensitivity

This study focusses on the environmental consequences of aircraft NOx emissions and their role and impact on ozone formation in the upper troposphere and lower stratosphere (UTLS). We use a global chemistry transport/box model approach to quantify the impacts of NOx on UTLS ozone over time scales of hours and over distance scales appropriate to air traffic corridors and aircraft flight paths. An important feature of our study has been to provide a marked contrast to the coarse spatial resolution of the global model studies typically employed to assess the impacts of aviation NOx on UTLS ozone. Real operational aviation routing data are used to quantify the NOx impacts on ozone at 235 locations on 21 flight paths. The NOx impact on ozone in the intercontinental air traffic corridors is strongest in the great circle routes from North America and Europe into Asia and weakest in the trans-polar routes. The NOx impacts identified with the CTM/box model combination are significantly smaller compared with those identified in the current global models typically used to assess aviation NOx impacts. Further research is required to confirm our assessment of those flight paths that appear to show greatest NOx – O3 impacts and those the least and extend our analyses into the tropics and southern hemisphere

Collaboration is key to strengthening surgical research capacity in sub-Saharan Africa

The paucity of research in areas of greatest clinical need must be addressed urgently. We propose a model of collaboration in an era of information systems and emerging mobile health technology that has had significant success across the UK and has shown early encouraging results in South Africa (SA). We foresee that recent examples of surgical research collaboratives in SA will continue to promote regional, national and international ‘hub-and-spoke’ models and ultimately increase the South-South collaboration that is urgently needed to diffuse the skills and knowledge required to address the unmet surgical need in sub-Saharan Africa

TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape.

Intracellular pathogens cause membrane distortion and damage as they enter host cells. Cells perceive these membrane alterations as danger signals and respond by activating autophagy. This response has primarily been studied during bacterial invasion, and only rarely in viral infections. Here, we investigate the cellular response to membrane damage during adenoviral entry. Adenoviruses and their vector derivatives, that are an important vaccine platform against SARS-CoV-2, enter the host cell by endocytosis followed by lysis of the endosomal membrane. We previously showed that cells mount a locally confined autophagy response at the site of endosomal membrane lysis. Here we describe the mechanism of autophagy induction: endosomal membrane damage activates the kinase TBK1 that accumulates in its phosphorylated form at the penetration site. Activation and recruitment of TBK1 require detection of membrane damage by galectin 8 but occur independently of classical autophagy receptors or functional autophagy. Instead, TBK1 itself promotes subsequent autophagy that adenoviruses need to take control of. Depletion of TBK1 reduces LC3 lipidation during adenovirus infection and restores the infectivity of an adenovirus mutant that is restricted by autophagy. By comparing adenovirus-induced membrane damage to sterile lysosomal damage, we implicate TBK1 in the response to a broader range of types of membrane damage. Our study thus highlights an important role for TBK1 in the cellular response to adenoviral endosome penetration and places TBK1 early in the pathway leading to autophagy in response to membrane damage

Temporal shifts in prokaryotic metabolism in response to organic carbon dynamics in the mesopelagic ocean during an export event in the Southern ocean

As the major term in downward organic carbon flux attenuation, determining prokaryotic metabolism over depth in the mesopelagic ocean is crucial for constraining the efficiency of the gravitational biological carbon pump (BCP). We hypothesize that the enhancement of particulate organic carbon (POC) concentrations in the mesopelagic twilight zone during export events leads to a temporally dynamic prokaryotic metabolic response, which likely has consequences for the efficiency of the BCP. We tested this hypothesis by making repeated measurements of leucine assimilation and leucine respiration at in situ concentrations over six depths throughout the upper 500 m of the water column during the collapse of a large-scale Southern Ocean spring diatom bloom. Rates of prokaryotic leucine assimilation were used to indicate levels of prokaryotic heterotrophic production, and leucine assimilation efficiency (LAE; the proportion of leucine used for growth versus respiration) was taken as an indicator of prokaryotic growth efficiency. Thus, relative shifts in LAE are indicative of shifts in rates of prokaryotic production relative to respiration. The flux of POC through the oceans’ interior led to a dynamic prokaryotic response, characterized by a temporary elevation in mesopelagic prokaryote leucine assimilation rates, LAE and prokaryotic abundance. By the final measurement these changes had already begun to revert, despite POC concentrations still being enriched. As hypothesized, our data revealed distinctions in the phases of the mesopelagic system, likely due to an evolution in bulk prokaryotic metabolic status and the amount and composition of organic matter available. This indicates that estimating ocean carbon sequestration during export events necessitates a time course of measurements throughout the period of POC downward flux. Our findings also revealed distinctions in the ecophysiological prokaryotic responses to substrate regimes between the surface mixed layer and the mesopelagic. Specifically, in the latter in situ leucine concentrations appeared more significant in controlling prokaryote metabolism than POC concentration, and were more closely related to per cell leucine assimilation, than respiration. Whereas, in the mixed layer, the concentration of in situ leucine did not seem to drive rates of its assimilation, rather POC concentration was a strong negative driver of cell specific leucine respiration. These findings are suggestive of stronger levels of energy limitation in the deeper ocean. We surmised that ocean regions with sporadic substrate supply to the mesopelagic are likely to experience stronger energy limitation which favors prokaryotic respiration over production