Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene

Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied

Phénotypes des isozymes érythrocytaires, phosphatase acide, phosphoglucomutase, adélynate kinase, adénosine désaminase, dans un échantillon de population de Dakar (Sénégal)

Summary. Red cell isozyme phenotypes were studied on a sample of 270 Negro blood donors living in the district of Dakar (Senegal). The gene frequencies were : — acid phosphatase : Pa 0.1963 ; Pb 0.7796 ; Pc 0.0037 ; Pr 0.0204 ; — Phosphoglucomutase : PGM\ 0.1944 ; — adenylate kinase : AK2 absent ; — adenosine deaminase : ADA2 absent (200 blood samples tested). Heterogeneity is suggested between ethnic groups, in the acid phospatase system.Résumé. Nous avons examiné un échantillon de 270 sujets sénégalais, non apparentés. Les fréquences observées pour les gènes des quatre systèmes étudiés sont : — phosphatase acide : Pa 0,1963 ; Pb 0,7796 ; Pc 0,0037 ; Pr 0,0204 ; — phosphoglucomutase : PGM2 0,1944 ; — adénylate kinase : absence de AK2 ; — adénosine désaminase : absence de ADA2 (200 échantillons). Une hétérogénéité tend à se manifester entre les divers groupes ethniques, particulièrement dans le système de phosphatase acide. Elle devra être confirmée sur un effectif plus important.Raymondjean M., Raymondjean Noémie, Diebolt Georgette, Linhard J., Moullec J. Phénotypes des isozymes érythrocytaires, phosphatase acide, phosphoglucomutase, adélynate kinase, adénosine désaminase, dans un échantillon de population de Dakar (Sénégal). In: Bulletins et Mémoires de la Société d'anthropologie de Paris, XIII° Série. Tome 2 fascicule 1, 1975. pp. 17-21