Kinetic tetrazolium microtiter assay

A method for conducting an in vitro cell assay using a tetrazolium indicator is disclosed. The indicator includes a nonionic detergent which solubilizes a tetrazolium reduction product in vitro and has low toxicity for the cells. The incubation of test cells in the presence of zolium bromide and octoxynol (TRITON X-100) permits kinetics of the cell metabolism to be determined

Shear flow in bends

October 1955Errata sheet inserted in frontAt head of title: Shear flow in bendsIncludes bibliographical references (page 12)Turbulent end wall boundary layers in the hub region of axial compressor blade rows have been studied in the Gas Turbine Laboratory. Both analytical and experimental work are presented. An attempt was made to predict the growth of the boundary layer momentum thickness as it passes through a cascade of blades using the momentum integral equation and several assumptions This calculation was compared with measurements taken along an assumed free streamline in a stationary cascade of blades supplied with an artificially skewed inlet boundary layer. From this comparison, the relative influence of individual terms in the momentum integral equation is deduced. The effects of inlet skewing and cross flow in the boundary layer in the region between blades is described. Two methods of representing main and cross flow velocity profiles are compared with experimental data.Under the sponsorship of: Office of Naval Research Contract N5ori 0784

Laboratory studies in ultraviolet solar physics

The research activity comprised the measurement of basic atomic processes and parameters which relate directly to the interpretation of solar ultraviolet observations and to the development of comprehensive models of the component structures of the solar atmosphere. The research was specifically directed towards providing the relevant atomic data needed to perform and to improve solar diagnostic techniques which probe active and quiet portions of the solar chromosphere, the transition zone, the inner corona, and the solar wind acceleration regions of the extended corona. The accuracy with which the physical conditions in these structures can be determined depends directly on the accuracy and completeness of the atomic and molecular data. These laboratory data are used to support the analysis programs of past and current solar observations (e.g., the Orbiting solar Observatories, the Solar Maximum Mission, the Skylab Apollo Telescope Mount, and the Naval Research Laboratory's rocket-borne High Resolution Telescope and Spectrograph). In addition, we attempted to anticipate the needs of future space-borne solar studies such as from the joint ESA/NASA Solar and Heliospheric Observatory (SOHO) spacecraft. Our laboratory activities stressed two categories of study: (1) the measurement of absolute rate coefficients for dielectronic recombination and electron impact excitation; and (2) the measurement of atomic transition probabilities for solar density diagnostics. A brief summary of the research activity is provided

Testable two-loop radiative neutrino mass model based on an LLQdcQdcLLQd^cQd^c effective operator

A new two-loop radiative Majorana neutrino mass model is constructed from the gauge-invariant effective operator $L^i L^j Q^k d^c Q^l d^c \epsilon_{ik} \epsilon_{jl}$ that violates lepton number conservation by two units. The ultraviolet completion features two scalar leptoquark flavors and a color-octet Majorana fermion. We show that there exists a region of parameter space where the neutrino oscillation data can be fitted while simultaneously meeting flavor-violation and collider bounds. The model is testable through lepton flavor-violating processes such as ${\mu} \to e{\gamma}$, $\mu \to eee$, and $\mu N \to eN$ conversion, as well as collider searches for the scalar leptoquarks and color-octet fermion. We computed and compiled a list of necessary Passarino-Veltman integrals up to boxes in the approximation of vanishing external momenta and made them available as a Mathematica package, denoted as ANT.Comment: 42 pages, 11 figures, typo in Eq. (4.9) as well as wrong chirality structures in Secs. 4.5 and 5.2 corrected, final results unchange

Pre-operational baseline studies of selected nearshore marine biota at the Diablo Canyon power plant site: 1979-1982

This is the final report of the California Department of Fish and Games intertidal and subtidal surveys of plants and animals in the vicinity of the Diablo Canyon Power Plant. These studies cover the period from 1979 through 1982. Our previous report (Gotshall, et al. 1984) covered the period from 1973 through 1978. The report includes abundances and statistical analyses of comparisons of abundances between years and study areas for selected intertidal and subtidal plants and animals. A total of 556 random subtidal stations, 540 intertidal stations and 67 permanent abalone transects were completed during this report period. Trends in abundances of most species observed during our 1973 through 1978 studies continued, i.e. the population of giant red sea urchins remained at a very low level, bull kelp Nereocystis leutkeana densities continued to decline in Diablo Cove and North Control. These two trends are probably due to the effects of continued sea otter foraging in the study area. Our observations of the presence or absence of fishes at subtidal 30m stations indicate a continued decline in the abundances of lingcod, Ophiodon elongatus and a decline in the abundance of blue rockfish since the 1973 through 1978 study period. A new study was begun during this study period, the use of baited stations to obtain relative abundance indices for those species of fishes attracted to the bait. Black-and-yellow rockfish were the most frequently observed fishes at Diablo Cove stations, while blue rockfish were the most frequently observed fish at North control baited stations. (Document has 393 pages

The off-axis channel macroplate

High-gain microchannel plates (MCPs) which utilize curvature of the channel to inhibit ion feedback (C-plate MCRs) have demonstrated excellent performance characteristics. However, C-plate MCPs are at present costly to fabricate, and the shearing process used to curve the channels produces a low device yield. Described here is a totally new type of high-gain MCP structure in which each channel has an axially symmetric curvature. Initial tests of proof-of-concept units of these MCPs with 75-micron-diameter channels (macroplates) suggest that their performance characteristics have the potential to be equal to those of a C-plate MCP while the fabrication process is no more complex than that of a conventional straight-channel MCP

Automatic estimation of flux distributions of astrophysical source populations

In astrophysics a common goal is to infer the flux distribution of populations of scientifically interesting objects such as pulsars or supernovae. In practice, inference for the flux distribution is often conducted using the cumulative distribution of the number of sources detected at a given sensitivity. The resulting "$\log(N>S)$-$\log (S)$" relationship can be used to compare and evaluate theoretical models for source populations and their evolution. Under restrictive assumptions the relationship should be linear. In practice, however, when simple theoretical models fail, it is common for astrophysicists to use prespecified piecewise linear models. This paper proposes a methodology for estimating both the number and locations of "breakpoints" in astrophysical source populations that extends beyond existing work in this field. An important component of the proposed methodology is a new interwoven EM algorithm that computes parameter estimates. It is shown that in simple settings such estimates are asymptotically consistent despite the complex nature of the parameter space. Through simulation studies it is demonstrated that the proposed methodology is capable of accurately detecting structural breaks in a variety of parameter configurations. This paper concludes with an application of our methodology to the Chandra Deep Field North (CDFN) data set.Comment: Published in at http://dx.doi.org/10.1214/14-AOAS750 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Longitudinal Monitoring of SARS-CoV-2 IgM and IgG Seropositivity to Detect COVID-19.

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a novel beta-coronavirus that has recently emerged as the cause of the 2019 coronavirus pandemic (COVID-19). Polymerase chain reaction (PCR) based tests are optimal and recommended for the diagnosis of an acute SARS-CoV-2 infection. Serology tests for viral antibodies provide an important tool to diagnose previous exposure to the virus. Here we evaluate the analytical performance parameters of the Diazyme SARS-CoV-2 IgM/IgG serology assays and describe the kinetics of IgM and IgG seroconversion observed in patients with PCR-confirmed COVID-19 who were admitted to our hospital.MethodsWe validated the performance of the Diazyme assay in 235 presumed SARS-CoV-2 negative subjects to determine specificity. Subsequently, we evaluated the SARS-CoV-2 IgM and IgG seroconversion of 54 PCR-confirmed COVID-19 patients and determined sensitivity of the assay at three different timeframes.ResultSensitivity and specificity for detecting seropositivity at ≥15 days following a positive SARS-CoV-2 PCR result, was 100.0% and 98.7% when assaying for the panel of IgM and IgG. The median time to seropositivity observed for a reactive IgM and IgG result from the date of a positive PCR was 5 days (IQR: 2.75-9 days) and 4 days (IQR: 2.75-6.75 days), respectively.ConclusionsOur data demonstrate that the Diazyme IgM/IgG assays are suited for the purpose of detecting SARS-CoV-2 IgG and IgM in patients with suspected SARS-CoV-2 infections. For the first time, we report longitudinal data showing the evolution of seroconversion for both IgG and IgM in a cohort of acutely ill patients in the United States. We also demonstrate a low false positive rate in patients who were presumed to be disease free