Lung cancer in never smokers (LCINS): development of a UK national research strategy

Introduction Lung cancer in never smokers (LCINS) accounts for 15% of lung cancers diagnosed in the UK, making it the 8th most common cancer. There are few robust studies specific to the LCINS population making data surrounding the incidence and mortality of LCINS incomplete, leaving many gaps in our understanding of the needs of this population. Methods To address a lack of research in this important area, the UK National Cancer Research Institute Lung Study Group (NCRI-LSG) undertook a national survey and hosted a research strategy day to define key research priorities. A wide cross section of stakeholders, including patient advocates, the charitable sector, basic and translational researchers, and multi-disciplinary healthcare professionals contributed highlighting their research priorities. Results One-hundred twenty-seven surveys were completed (52 by patients/patient advocates) prior to the strategy day. These identified themes for expert review presentations and subsequent workshop discussions at the national research strategy day, which registered 190 attendees (50 patients/patient advocates). The four key themes that emerged to form the basis of a research strategy for LCINS are (1) Raising awareness, (2) Risk assessment and early detection, (3) Disease biology, (4) Living with and beyond. Conclusion This paper summarises current evidence and important gaps in our knowledge related to LCINS. We present recommendations for a national research strategy aimed at improving outcomes for patients

Comorbidities and the referral pathway to access joint replacement surgery: an exploratory qualitative study

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The research was funded by the National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care North Thames (CLAHRC) at Barts Health NHS Trust

Untargeted gas chromatography–mass spectrometry-based metabolomics analysis of kidney and liver tissue from the Lewis Polycystic Kidney rat

Polycystic kidney disease (PKD) encompasses a spectrum of inherited disorders that lead to end-stage renal disease (ESRD). There is no cure for PKD and current treatment options are limited to renal replacement therapy and transplantation. A better understanding of the pathobiology of PKD is needed for the development of new, less invasive treatments. The Lewis Polycystic Kidney (LPK) rat phenotype has been characterized and classified as a model of nephronophthisis (NPHP9, caused by mutation of the Nek8 gene) for which polycystic kidneys are one of the main pathologic features. The aim of this study was to use a GC–MS-based untargeted metabolomics approach to determine key biochemical changes in kidney and liver tissue of the LPK rat. Tissues from 16-week old LPK (n = 10) and Lewis age- and sex-matched control animals (n = 11) were used. Principal component analysis (PCA) distinguished signal corrected metabolite profiles from Lewis and LPK rats for kidney (PC-1 77%) and liver (PC-1 46%) tissue. There were marked differences in the metabolite profiles of the kidney tissues with 122 deconvoluted features significantly different between the LPK and Lewis strains. The metabolite profiles were less marked between strains for liver samples with 30 features significantly different. Five biochemical pathways showed three or more significantly altered metabolites: transcription/translation, arginine and proline metabolism, alpha-linolenic and linoleic acid metabolism, the citric acid cycle, and the urea cycle. The results of this study validate and complement the current literature and are consistent with the understood pathobiology of PKD

Caracterização do solo de cobertura de aterros encerrados com ferramentas (geo)estatísticas Characterization of soil covers in closed landfill sites with (geo)statistical tools

Inúmeros trabalhos abordam a elaboração de estratégias amostrais e a aplicação de ferramentas (geo)estatísticas no estudo de atributos do solo. Entretanto, são escassos os trabalhos envolvendo a aplicação desta abordagem no monitoramento de solos construídos sobre aterros encerrados de resíduos sólidos urbanos. Este estudo mostra que a densidade amostral necessária para tornar possível o uso da geoestatística em tais casos, elevaria os custos operacionais. A melhor alternativa é a utilização dos métodos de estatística multivariada (análise de componentes principais e de agrupamento) para definição de zonas homogêneas de manejo. Os atributos que melhor explicam a estrutura da variabilidade do solo construído são o teor de areia (ou argila), a saturação por bases e o pH, todos relacionados com a contaminação do solo com chorume e o adequado desenvolvimento da vegetação.<br>Several studies address the development of sampling strategies and implementation of (geo)statistical tools in the study of soil properties. However, there is a lack of studies in the application of such approach to monitor soil covers in closed landfill sites of urban solid waste. This study shows that the sampling density needed to make possible the use of geostatistics in such cases would raise operational costs. The best alternative is the use of multivariate statistics methods (principal components and cluster analysis) to define homogeneous management zones. The soil attributes that best explain the structure of soil variability are sand (or clay) content, base saturation and pH, all related with soil contamination by leachate and with the proper development of vegetation