131 research outputs found
Etching effects during the chemical vapor deposition of (100) diamond
Current theories of CVD growth on (100) diamond are unable to account for the numerous experimental observations of slow-growing, locally smooth (100)(2×1)(100)(2×1) films. In this paper we use quantum mechanical calculations of diamond surface thermochemistry and atomic-scale kinetic Monte Carlo simulations of deposition to investigate the efficacy of preferential etching as a mechanism that can help to reconcile this discrepancy. This etching mechanism allows for the removal of undercoordinated carbon atoms from the diamond surface. In the absence of etching, simulated growth on the (100)(2×1)(100)(2×1) surface is faster than growth on the (110) and (111) surfaces, and the (100) surface is atomically rough. When etching is included in the simulations, the (100) growth rates decrease to values near those observed experimentally, while the rates of growth on the other surfaces remain largely unaffected and similar to those observed experimentally. In addition, the etching mechanism promotes the growth of smooth (100) surface regions in agreement with numerous scanning probe studies. © 1999 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70606/2/JCPSA6-111-9-4291-1.pd
Expression and Function of Ccbe1 in the Chick Early Cardiogenic Regions Are Required for Correct Heart Development
During the course of a differential screen to identify transcripts specific for chick heart/hemangioblast precursor cells, we have identified Ccbe1 (Collagen and calcium-binding EGF-like domain 1). While the importance of Ccbe1 for the development of the lymphatic system is now well demonstrated, its role in cardiac formation remained unknown. Here we show by whole-mount in situ hybridization analysis that cCcbe1 mRNA is initially detected in early cardiac progenitors of the two bilateral cardiogenic fields (HH4), and at later stages on the second heart field (HH9-18). Furthermore, cCcbe1 is expressed in multipotent and highly proliferative cardiac progenitors. We characterized the role of cCcbe1 during early cardiogenesis by performing functional studies. Upon morpholino-induced cCcbe1 knockdown, the chick embryos displayed heart malformations, which include aberrant fusion of the heart fields, leading to incomplete terminal differentiation of the cardiomyocytes. cCcbe1 overexpression also resulted in severe heart defects, including cardia bifida. Altogether, our data demonstrate that although cardiac progenitors cells are specified in cCcbe1 morphants, the migration and proliferation of cardiac precursors cells are impaired, suggesting that cCcbe1 is a key gene during early heart development.FCT [SFRH/BD/65628/2009, SFRH/BPD/86497/2012, SFRH/BPD/41081/2007]; F.C.T.B.I. fellowship [PTDC/SAU-BID/114902/ 2009]; FCT; Institute for Biotechnology Bioengineering (Centro Biomedicina Molecular e Celular (IBB/CBME), Laboratorio Associado (LA) in the frame of Project [PestOE/EQB/LA0023/2013]info:eu-repo/semantics/publishedVersio
A strategy to discover new organizers identifies a putative heart organizer
Organizers are regions of the embryo that can both induce new fates and impart pattern on other regions. So far, surprisingly few organizers have been discovered, considering the number of patterned tissue types generated during development. This may be because their discovery has relied on transplantation and ablation experiments. Here we describe a new approach, using chick embryos, to discover organizers based on a common gene expression signature, and use it to uncover the anterior intestinal portal (AIP) endoderm as a putative heart organizer. We show that the AIP can induce cardiac identity from non-cardiac mesoderm and that it can pattern this by specifying ventricular and suppressing atrial regional identity. We also uncover some of the signals responsible. The method holds promise as a tool to discover other novel organizers acting during development
Recommended from our members
A Novel Method of Evaporation Suppression in a Water Harvesting System
From the Proceedings of the 1984 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona-Nevada Academy of Science - April 7, 1984, Flagstaff, ArizonaA demonstration agrisystem located in an area receiving less than 250 mm rainfall annually has been constructed through a cooperative program between the City of Tucson and the University of Arizona. Mondell pine, aleppo pine, jojoba, grapes, eucalyptus, olives, and other crops were cultivated in a 4 ha NaC1 treated catchment system designed to concentrate rainfall on plants and channel excess water into a system of storage reservoirs. Evaporation was reduced from an 80 foot diameter above ground reservoir by means of 225,000 plastic film cans, at a cost of approximately 50 cents /ft². Data acquired from evaporation pans indicates a 50 to 70 percent reduction in evaporation of the stored water. Additionally, this research has provided data that 1) demonstrates the economic potential for agriculture of currently retired farmland, 2) investigates the feasibility of applying water harvesting method for agricultgural purposes in a semiarid region, and 3) evaluates water harvesting as an alternative to meet the ever increasing demand for water.This article is part of the Hydrology and Water Resources in Arizona and the Southwest collections. Digital access to this material is made possible by the Arizona-Nevada Academy of Science and the University of Arizona Libraries. For more information about items in this collection, contact [email protected]
Recommended from our members
Water Harvesting: An Alternative Use for Retired Farmlands
From the Proceedings of the 1983 Meetings of the Arizona Section - American Water Resources Assn. and the Hydrology Section - Arizona-Nevada Academy of Science - April 16, 1983, Flagstaff, ArizonaThis article is part of the Hydrology and Water Resources in Arizona and the Southwest collections. Digital access to this material is made possible by the Arizona-Nevada Academy of Science and the University of Arizona Libraries. For more information about items in this collection, contact [email protected]
Biomass Potential in Arizona
Desert Plants is published by The University of Arizona for the Boyce Thompson Southwestern Arboretum. For more information about this unique botanical journal, please email the College of Agriculture and Life Sciences Publications Office at [email protected]
- …