Complete plant regeneration of Valeriana wallichii DC. on auxin enriched medium and phytochemical analysis

Valeriana wallichii DC. (Valerianaceae) is a well-known medicinal herb distributed in Northwest Himalayas. The herb is utilized in the treatment of numerous ailments and diseases such as diarrhoea, diabeties, gastrospans, ulcer and wound healing, etc. Overexploitation, especially collection of rhizomes on a large scale for the medicinal purpose has significantly declined the availability of the herb in natural stands. Hence, there is a requirement of development of cultivation practices and protocol for mass propagation to achieve sustained utilization along with conservation of the species. In the present study, in-vitro culture of nodal segments on MS+2iP+IAA+2,4-D and MS+2iP+IAA+NAA resulted in multiple shoot induction along with regeneration of in-vitro roots on the same medium making the protocol cost-effective, efficient and comparatively less time-consuming. Moreover, the regeneration of adventitious roots from regenerated shoots enhanced the total number of plants obtained per explant as shoots with adventitious roots were individually excised and were transferred to natural conditions through the process of acclimatization. GC-MS analysis of a methanolic extract of leaves of the mother plant and micropropagated plant revealed the presence of 37 and 36 phytocompounds respectively. Phytocompounds including eucalyptol, neophytidiene, hexadecenoic acid, dimethyl palmitamine were identified to be present in the leaves of both mother and micropropagated plants whereas other compounds such as eicosyne, lilial, behenic alcohol were confined to be present in extract prepared from leaves of mother plant. Similary some phytocompounds (phytol, vinicizer, retinol) were detected in methanolic extract prepared from leaves of micropropagated plants

First aid knowledge among health assigned teachers of primary schools

Background: Every parents of a child knows that it is hard enough to keep their child safe at home but when child goes off to school there is a whole new set up of challenges. Parents totally entrust their children to the schools. They rely on the teachers as their second parents in the institute for learning. They know for a fact that their educators will want no harm to come their way. While they are busy working, they fully believe that their children are well cared for. On the other hand, the teachers have the responsibility to keep an eye on their students. They should make sure that they are comfortable and secured within the four walls of their school. This then calls for their ability to handle even the most stressful situations. So much more, the school staff, especially the health teacher has to be equipped with the knowledge of first aid.Methods: Non-experimental survey study was conducted to assess the knowledge of first-aid among Health assigned teacher working in selected schools of Dehradun city. Using purposive sampling technique 40 samples of different schools of Dehradun city was adopted to select the area.Results: Result shows that the majority of health assign teachers were having average knowledge about first aid i.e. 29 (72.5%) and 10 (25%) were having good knowledge and 1 (2.5%) was having poor knowledge regarding first aid. Majority of participant were female 21 (52.5%). The educational status of most participants was post graduate (67.5%). Majority of the schools were private 25 (62.5%). There is no significant association between socio-demographic variables i.e. Gender, level of schools, year of experience as a health assigned teacher and education provided by teacher, but qualifications of teachers and type of schools was found to have a significant association.(p> 0.05).Conclusions: First aid is the assistance given to any person suffering a sudden illness or injury, with care it is provided to preserve life and to prevent the condition from worsening, and for promoting recovery. It is important that health assigned teachers are equipped with strong, professional competencies. In order for health promotion actions to be sustainable in schools, teachers must be capable, competent and skilled health educators. As they are at prime position to contribute to a nations health gain through the provision of health education for future adults. Based on findings we can say that the health assigned teachers are having average knowledge regarding first aid. Therefore, the health assigned teachers must be equipped with appropriate and upgraded knowledge

Substrate Binding Mode and Its Implication on Drug Design for Botulinum Neurotoxin A

The seven antigenically distinct serotypes of Clostridium botulinum neurotoxins, the causative agents of botulism, block the neurotransmitter release by specifically cleaving one of the three SNARE proteins and induce flaccid paralysis. The Centers for Disease Control and Prevention (CDC) has declared them as Category A biowarfare agents. The most potent among them, botulinum neurotoxin type A (BoNT/A), cleaves its substrate synaptosome-associated protein of 25 kDa (SNAP-25). An efficient drug for botulism can be developed only with the knowledge of interactions between the substrate and enzyme at the active site. Here, we report the crystal structures of the catalytic domain of BoNT/A with its uncleavable SNAP-25 peptide 197QRATKM202 and its variant 197RRATKM202 to 1.5 Å and 1.6 Å, respectively. This is the first time the structure of an uncleavable substrate bound to an active botulinum neurotoxin is reported and it has helped in unequivocally defining S1 to S5′ sites. These substrate peptides make interactions with the enzyme predominantly by the residues from 160, 200, 250 and 370 loops. Most notably, the amino nitrogen and carbonyl oxygen of P1 residue (Gln197) chelate the zinc ion and replace the nucleophilic water. The P1′-Arg198, occupies the S1′ site formed by Arg363, Thr220, Asp370, Thr215, Ile161, Phe163 and Phe194. The S2′ subsite is formed by Arg363, Asn368 and Asp370, while S3′ subsite is formed by Tyr251, Leu256, Val258, Tyr366, Phe369 and Asn388. P4′-Lys201 makes hydrogen bond with Gln162. P5′-Met202 binds in the hydrophobic pocket formed by the residues from the 250 and 200 loop. Knowledge of interactions between the enzyme and substrate peptide from these complex structures should form the basis for design of potent inhibitors for this neurotoxin

Novel realization of GIC based wave digital filters using Al-Alaoui transform

By using the concepts of wave characterization, Al-Alaoui transform and generalized immittance converter (GIC), a new digital GIC structure is proposed which is capable of realizing all the standard second-order digital filter sections. The Al-Alaoui transform is utilized for reference circuit discretization, leading to development of second-order digital filter sections which possess better noise properties than the conventional GIC and GIC discretized using the fractional bilinear transform. The proposed second-order digital filter sections are used as building blocks in cascade synthesis of filter. Two design examples are considered to analyze the effectiveness of the proposed filter sections and their comparison with the other filter sections is performed in terms of quantization noise and approximation to analog filter response. The proposed synthesis yields lowpass and highpass filters that have a better signal to noise ratio and better approximation of the analog filter frequency response than the conventional GIC digital filters and fractional GIC digital filters. Keywords: Digital filter, Al-Alaoui transform, Generalized immittance converter, Wave equation

Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis

Abstract Background Cyclopropane fatty acids (CPA) have been found in certain gymnosperms, Malvales, Litchi and other Sapindales. The presence of their unique strained ring structures confers physical and chemical properties characteristic of unsaturated fatty acids with the oxidative stability displayed by saturated fatty acids making them of considerable industrial interest. While cyclopropenoid fatty acids (CPE) are well-known inhibitors of fatty acid desaturation in animals, CPE can also inhibit the stearoyl-CoA desaturase and interfere with the maturation and reproduction of some insect species suggesting that in addition to their traditional role as storage lipids, CPE can contribute to the protection of plants from herbivory. Results Three genes encoding cyclopropane synthase homologues GhCPS1, GhCPS2 and GhCPS3 were identified in cotton. Determination of gene transcript abundance revealed differences among the expression of GhCPS1, 2 and 3 showing high, intermediate and low levels, respectively, of transcripts in roots and stems; whereas GhCPS1 and 2 are both expressed at low levels in seeds. Analyses of fatty acid composition in different tissues indicate that the expression patterns of GhCPS1 and 2 correlate with cyclic fatty acid (CFA) distribution. Deletion of the N-terminal oxidase domain lowered GhCPS's ability to produce cyclopropane fatty acid by approximately 70%. GhCPS1 and 2, but not 3 resulted in the production of cyclopropane fatty acids upon heterologous expression in yeast, tobacco BY2 cell and Arabidopsis seed. Conclusions In cotton GhCPS1 and 2 gene expression correlates with the total CFA content in roots, stems and seeds. That GhCPS1 and 2 are expressed at a similar level in seed suggests both of them can be considered potential targets for gene silencing to reduce undesirable seed CPE accumulation. Because GhCPS1 is more active in yeast than the published Sterculia CPS and shows similar activity when expressed in model plant systems, it represents a strong candidate gene for CFA accumulation via heterologous expression in production plants.</p

Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis

BACKGROUND: Cyclopropane fatty acids (CPA) have been found in certain gymnosperms, Malvales, Litchi and other Sapindales. The presence of their unique strained ring structures confers physical and chemical properties characteristic of unsaturated fatty acids with the oxidative stability displayed by saturated fatty acids making them of considerable industrial interest. While cyclopropenoid fatty acids (CPE) are well-known inhibitors of fatty acid desaturation in animals, CPE can also inhibit the stearoyl-CoA desaturase and interfere with the maturation and reproduction of some insect species suggesting that in addition to their traditional role as storage lipids, CPE can contribute to the protection of plants from herbivory. RESULTS: Three genes encoding cyclopropane synthase homologues GhCPS1, GhCPS2 and GhCPS3 were identified in cotton. Determination of gene transcript abundance revealed differences among the expression of GhCPS1, 2 and 3 showing high, intermediate and low levels, respectively, of transcripts in roots and stems; whereas GhCPS1 and 2 are both expressed at low levels in seeds. Analyses of fatty acid composition in different tissues indicate that the expression patterns of GhCPS1 and 2 correlate with cyclic fatty acid (CFA) distribution. Deletion of the N-terminal oxidase domain lowered GhCPS's ability to produce cyclopropane fatty acid by approximately 70%. GhCPS1 and 2, but not 3 resulted in the production of cyclopropane fatty acids upon heterologous expression in yeast, tobacco BY2 cell and Arabidopsis seed. CONCLUSIONS: In cotton GhCPS1 and 2 gene expression correlates with the total CFA content in roots, stems and seeds. That GhCPS1 and 2 are expressed at a similar level in seed suggests both of them can be considered potential targets for gene silencing to reduce undesirable seed CPE accumulation. Because GhCPS1 is more active in yeast than the published Sterculia CPS and shows similar activity when expressed in model plant systems, it represents a strong candidate gene for CFA accumulation via heterologous expression in production plants

<span style="font-size:11.0pt;font-family: "Times New Roman","serif";mso-fareast-font-family:"Times New Roman";mso-bidi-font-family: Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language: HI" lang="EN-GB">Botanical and phytochemical studies on roots of <i>Berberis umbellata</i> Wall. ex G. Don</span>

55-60<i style="mso-bidi-font-style: normal">Berberis umbellata Wall. ex G. Don (Family—Berberidaceae) commonly known as Himalayan Barberry is an important medicinal plant used in Ayurvedic System of Medicine for treating eye disorders, fever, jaundice, skin diseases, etc. The present study provides macro- and microscopical characters, physico-chemical details such as total ash, acid-insoluble ash, hexane, alcohol and water soluble extractive, tannins, sugar and starch percentages. The percentage of berberine as berberine chloride was calculated through HPTLC densitometric method is 3.05%. Study has been undertaken to develop pharmacopoeial standards for B. umbellata, this will be utilized by researchers for exploring the possibility as a substitute to B. aristata DC., an official species in the name of Daruharidra reported in Ayurvedic Pharmacopoeia of India for herbal drug industry