Installation of New CSD (Carbonated Soft Drink) RGB (Returnable Glass Bottle) Line with Food Safety Comply and Final Validation

The basic of this project was installation of new CSD (carbonated soft drink) RGB (returnable glass bottle) line with comply food safety and final validation of line. New manufacturing line installation deals with installation of all equipment and machine require for smooth running and producing CSD for COCA-COLA company (Coca-Cola, Thums UP, Limca) such as conveyer belt, uncasing machine, light inspection station, bottle washer EBI (electronic bottle inspection ) machine, Paramix, Filler, Sealer, Date coding machine, caser etc. During installation of machine we have fulfill requirement related to machine, work space, hygienic condition so that machine can work smoothly and deliver safe food product The main concern of doing this project was deliver a safe product to the consumer by applying HACCP and ISO22K. I have done hazard analysis and validation of this new installed line and identify CCP and OPRP which need to control by applying control measure

Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis

Human papillomavirus induced (HPV+) cancer incidence is rapidly rising, comprising 60–80% of oropharyngeal squamous cell carcinomas (OPSCCs); while rare, recurrent/metastatic disease accounts for nearly all related deaths. An in vivo pre-clinical model for these invasive cancers is necessary for testing new therapies. We characterize an immune competent recurrent/metastatic HPV+ murine model of OPSSC which consists of four lung metastatic (MLM) cell lines isolated from an animal with HPV+ OPSCC that failed cisplatin/radiation treatment. These individual metastatic clonal cell lines were tested to verify their origin (parental transgene expression and define their physiological properties: proliferation, metastatic potential, heterogeneity and sensitivity/resistance to cisplatin and radiation. All MLMs retain expression of parental HPV16 E6 and E7 and degrade P53 yet are heterogeneous from one another and from the parental cell line as defined by Illumina expression microarray. Consistent with this, reverse phase protein array defines differences in protein expression/activation between MLMs as well as the parental line. While in vitro growth rates of MLMs are slower than the parental line, in vivo growth of MLM clones is greatly enhanced. Moreover, in vivo resistance to standard therapies is dramatically increased in 3 of the 4 MLMs. Lymphatic and/or lung metastasis occurs 100% of the time in one MLM line. This recurrent/metastatic model of HPV+ OPSCC retains the characteristics evident in refractory human disease (heterogeneity, resistance to therapy, metastasis in lymph nodes/lungs) thus serving as an ideal translational system to test novel therapeutics. Moreover, this system may provide insights into the molecular mechanisms of metastasis

Scaling Up Primary Health Services in Rural Tamil Nadu: Public Investment Requirements and Health Sector Reform

We attempt to address two key questions in this paper: 1) In terms of state-wide scaling up of rural services in the area of primary health, what will it cost financially and in terms of human resources to scale-up these services in all the rural areas of the state? And 2) what policy, institutional and governance reforms may be necessary so as to ensure proper service delivery? As is well known, merely setting up more health clinics, for instance, is not going to be enough; higher public investments in these areas needs to be accompanied by systemic reforms that will help overhaul the present service delivery system, including issues of control and oversight, for example

Scaling Up Primary Education Services in Rural Tamil Nadu: Public Investment Requirements and Reform

We attempt to address two key questions in this paper: 1) In terms of state-wide scaling up of rural services in the area of primary education, what will it cost financially and in terms of human resources to scale-up these services in all the rural areas of the state? And 2) What policy, institutional and governance reforms may be necessary so as to ensure proper service delivery? As is well known, merely constructing more schools, for instance, is not going to be enough; higher public investments in these areas needs to be accompanied by systemic reforms that will help overhaul the present service delivery system, including issues of control and oversight, for example

The propensity of molecules to spatially align in intense light fields

The propensity of molecules to spatially align along the polarization vector of intense, pulsed light fields is related to readily-accessible parameters (molecular polarizabilities, moment of inertia, peak intensity of the light and its pulse duration). Predictions can now be made of which molecules can be spatially aligned, and under what circumstances, upon irradiation by intense light. Accounting for both enhanced ionization and hyperpolarizability, it is shown that {\it all} molecules can be aligned, even those with the smallest static polarizability, when subjected to the shortest available laser pulses (of sufficient intensity).Comment: 8 pages, 4 figures, to be submitted to PR

Digitized archive of the Kodaikanal images: Representative results of solar cycle variation from sunspot area determination

Photographic images are valuable data resources for studying long term changes in the solar magnetic field and its influence on the Earth's climate and weather. We digitized more than 100 years of white light images stored in photographic plates and films that are available at Kodaikanal observatory starting from 1904. The digitized images were calibrated for relative plate density and aligned in such a way that the solar north is in upward direction. A semi-automated sunspot detection technique was used to identify the sunspots on the digitized images. In addition to describing the calibration procedure and availability of the data, we here present preliminary results on the sunspot area measurements and their variation with time. The results show that the white-light images have a uniform spatial resolution throughout the 90 years of observations. However, the contrast of the images decreases from 1968 onwards. The images are circular and do not show any major geometrical distortions. The measured monthly averaged sunspot areas closely match the Greenwich sunspot area over the four solar cycles studied here. The yearly averaged sunspot area shows a high degree of correlation with the Greenwich sunspot area. Though the monthly averaged sunspot number shows a good correlation with the monthly averaged sunspot areas, there is a slight anti-correlation between the two during solar maximum The Kodaikanal data archive is hosted at http://kso.iiap.res.in. The long time sequence of the Kodaikanal white light images provides a consistent data set for sunspot areas and other proxies. Many studies can be performed using Kodaikanal data alone without requiring intercalibration between different data sources.Comment: 9 pages, A&A(accepted

Physisorption of Nucleobases on Graphene

We report the results of our first-principles investigation on the interaction of the nucleobases adenine (A), cytosine (C), guanine (G), thymine (T), and uracil (U) with graphene, carried out within the density functional theory framework, with additional calculations utilizing Hartree--Fock plus second-order Moeller-Plesset perturbation theory. The calculated binding energy of the nucleobases shows the following hierarchy: G > T ~ C ~ A > U, with the equilibrium configuration being very similar for all five of them. Our results clearly demonstrate that the nucleobases exhibit significantly different interaction strengths when physisorbed on graphene. The stabilizing factor in the interaction between the base molecule and graphene sheet is dominated by the molecular polarizability that allows a weakly attractive dispersion force to be induced between them. The present study represents a significant step towards a first-principles understanding of how the base sequence of DNA can affect its interaction with carbon nanotubes, as observed experimentally.Comment: 7 pages, 3 figure

Polycistronic Delivery of IL-10 and NT-3 Promotes Oligodendrocyte Myelination and Functional Recovery in a Mouse Spinal Cord Injury Model.

One million estimated cases of spinal cord injury (SCI) have been reported in the United States and repairing an injury has constituted a difficult clinical challenge. The complex, dynamic, inhibitory microenvironment postinjury, which is characterized by proinflammatory signaling from invading leukocytes and lack of sufficient factors that promote axonal survival and elongation, limits regeneration. Herein, we investigated the delivery of polycistronic vectors, which have the potential to coexpress factors that target distinct barriers to regeneration, from a multiple channel poly(lactide-co-glycolide) (PLG) bridge to enhance spinal cord regeneration. In this study, we investigated polycistronic delivery of IL-10 that targets proinflammatory signaling, and NT-3 that targets axonal survival and elongation. A significant increase was observed in the density of regenerative macrophages for IL-10+NT-3 condition relative to conditions without IL-10. Furthermore, combined delivery of IL-10+NT-3 produced a significant increase of axonal density and notably myelinated axons compared with all other conditions. A significant increase in functional recovery was observed for IL-10+NT-3 delivery at 12 weeks postinjury that was positively correlated to oligodendrocyte myelinated axon density, suggesting oligodendrocyte-mediated myelination as an important target to improve functional recovery. These results further support the use of multiple channel PLG bridges as a growth supportive substrate and platform to deliver bioactive agents to modulate the SCI microenvironment and promote regeneration and functional recovery. Impact statement Spinal cord injury (SCI) results in a complex microenvironment that contains multiple barriers to regeneration and functional recovery. Multiple factors are necessary to address these barriers to regeneration, and polycistronic lentiviral gene therapy represents a strategy to locally express multiple factors simultaneously. A bicistronic vector encoding IL-10 and NT-3 was delivered from a poly(lactide-co-glycolide) bridge, which provides structural support that guides regeneration, resulting in increased axonal growth, myelination, and subsequent functional recovery. These results demonstrate the opportunity of targeting multiple barriers to SCI regeneration for additive effects

Starter culture technology: fermented foods

Practice of starter culture is an age old practice but without any scientific basis. With the awareness of need of microbial inoculations for regulation of fermentation process for quality production of desired quality foods innovative development have taken place. Development of new strains for elite strain which can improve the quality, stability, flavor, texture and phage resistant starter cultures are raised on a variety of medium depending organism as well as product to be developed. The starter culture are classified based on their composition growth requirement and methods of propagation. These starter cultures are preserved by different methods they can be available all the time. Though commercial strains are available their isolation and subsequent improvement through different genetical methods are desirable to get a novel strains with unique properties of commercial importance. Among different microorganisms such as lactic acid bacteria, yeast (Saccharomyce cerevisae, Penicillium camembertii, P. roquefortii) and Rhyzopus (R. oryzae, R. sojae) are extensively used for this purpose. Perfection in the preparation, storage and propagation need to be improved. Mixed starters with symbiotic activity have to be formulated