Synthesis of Al and Ag nanoparticles through ultra-sonic dissociation of thermal evaporation deposited thin films for promising clinical applications as polymer nanocomposite

Nanoparticles (NPs) having well-defined shape, size and clean surface serve as ideal model system to investigate surface/interfacial reactions. Ag and Al NPs are receiving great interest due to their wide applications in bio-medical field, aerospace and space technology as combustible additives in propellants and hydrogen generation. Hence, in this study, we have synthesized Ag and Al NPs using an innovative approach of ultra-sonic dissociation of thin films. Phase and particle size distributions of the Ag and Al NPs have been determined by X-ray diffraction (XRD) and transmission electron microscopy (TEM). Thin film dissociation/dissolution mechanism, hence conversion into NPs has been characterized by SEM- scanning electron microscope. EDXA & ICPMS have been performed for chemical analysis of NPs. Optical properties have been characterized by UV-Vis and PL spectroscopy. These NPs have also been investigated for their anti-bacterial activity against Escherichia coli bacteria. To the best of our knowledge, this is the first time when NPs has been synthesized by ultra-sonic dissociation of thin films. As an application, these NPs were used further for synthesis of nanocomposite polymer membranes, which show excellent activity against bio film formation

Economics of BN Hybrid Grass Production Bundelkhand Region of India: A Case Study

Napier grass is also called as Elephant Grass due to its tallness and vigorous vegetative growth. The plants tiller freely and a single clump may produce more than 60 tillers under favorable climatic and soil conditions. Unfortunately, the grass coarse-textured, the leaf blade sheaths as well as sharply serrated, leaf margins. Therefore, cross was made between Bajra which is more succulent, leafy, fine-textured, palatable, fast growing and drought resistant and Napier to combine these qualities with its high yielding potential. The outcome Hybrid Napier is a perennial grass which can be retained on field for 2-3 years. Hybrid napier grass are mostly grown under assured water supply but cultivating under varying agroclimatic condition is also possible. Low grass production in dry land area is mainly due to the limited availability of soil moisture and plant nutrients. Agrawal et al., (2001) reported NB hybrid is superior to guinea grass or setaria grass. However the economy of production plays an important role in introduction/ adoption of a crop in an area, village or farm. Many earlier studies have been conducted on economics of BN Hybrid production at research farm, or other government farms. But the information on economics of BN hybrid production at farmer’s field and its integration livestock feeding as well rooted slips are limited. Therefore, the present study was conducted with the object to study the economics of BN hybrid at farmer’s field in Bundelkhand

Rejuvenation of Wild Ber (\u3cem\u3eZizyphus\u3c/em\u3e Species) through Budding at Farmer’s Field

Ber is mainly cultivated for its fresh fruits, which are rich in vitamins C, A and B complex. Due to its nutritional qualities it is called poor man’s apple (Gajbhiya et al., 2003). A large number of wild ber shrubs (Zizyphus mauritiana, Z. rotundifolia and Z. nummularia) were seen growing on the neglected lands, uncultivated lands, roadsides and farm boundaries in Kadesara Kala Village in Lalitpur district of Uttar Pradesh (India). Production potential of this species is very low because of poor upkeep and inferior genetic makeup. But they have extensive root system which can withstand prolonged drought and has capability to regenerate even if the shoot system is damaged completely (Batahr, 1990). They are also responsibly resistant to common insect and pest. Therefore, these species offer an excellent production potential from the degraded lands of Bundelkhand region of India (Sharma and Tiwari, 1994; Tiwari and Sharma, 1993) after in-situ budding with improved cultivars. It also provides sufficient leaf biomass (pala) which can be utilized as fodder in lean period and also suitable for hay and silage for goat as it contains 11-13 percent crude protein (Tewatia and Khirwar, 2002). Pruned twigs/stems of ber also meet, to some extent, the fuel wood requirement in rural areas (Kumar et al., 2004)

Searching for Dark Matter with a Superconducting Qubit

Detection mechanisms for low mass bosonic dark matter candidates, such the axion or hidden photon, leverage potential interactions with electromagnetic fields, whereby the dark matter (of unknown mass) on rare occasion converts into a single photon. Current dark matter searches operating at microwave frequencies use a resonant cavity to coherently accumulate the field sourced by the dark matter and a near standard quantum limited (SQL) linear amplifier to read out the cavity signal. To further increase sensitivity to the dark matter signal, sub-SQL detection techniques are required. Here we report the development of a novel microwave photon counting technique and a new exclusion limit on hidden photon dark matter. We operate a superconducting qubit to make repeated quantum non-demolition measurements of cavity photons and apply a hidden Markov model analysis to reduce the noise to 15.7 dB below the quantum limit, with overall detector performance limited by a residual background of real photons. With the present device, we perform a hidden photon search and constrain the kinetic mixing angle to $\epsilon \leq 1.68 \times 10^{-15}$ in a band around 6.011 GHz (24.86 $\mu$eV) with an integration time of 8.33 s. This demonstrated noise reduction technique enables future dark matter searches to be sped up by a factor of 1300. By coupling a qubit to an arbitrary quantum sensor, more general sub-SQL metrology is possible with the techniques presented in this work.Comment: 15 pages, 14 figures, 2 table. Dark matter exclusion analysis modified to include experimental systematics. Discussion of background calibration and detector compatibility with tunable cavity added to conclusion. Future optimizations and integration into axion search sections moved to Supplemental Material. References update

Constraints for Grassland Development in Kadesara Kala Village, Lalitpur, Bundelkhand: A Case Study

India is maintaining a livestock population of over 500 million and has a grim situation of forage and feed deficit to the tune of 35.6% green fodder, 10.95% crop residues and 44% concentrate. In term of nutrients, the deficit is expected to the tune of 24.6 and 19.9% respectively by the year 2020. The gap is to be bridged up through development as well as extension of appropriate fodder production technologies suitable for different farming situation strategies. Fifty-eight % of the available fodder comes from grazing land and 42% from stall feeding with crop residues (30%) and leaf fodder (12%). Grasses constitute the majority (88%) of fodder available in Uttar Pradesh (GOUP, 1994). However, the role of grasslands cannot be under judged, as the large animal populations owned by small, marginal and landless farm family strive upon these. The livestock sector achieved an average growth rate of 4.8 per cent during the Eleventh Five Year Plan (Economic Survey, 2012-13) and it contributed 3.6 percent of national GDP during the Eleventh Plan. In the same tune, the contribution from this sector is expected to improve because increasing purchasing power is favoring the proportion of protein from animal source in the human diets. Further, The Indian livestock sector is becoming more competitive participant in the world market. All this largely depends, however, on improvement and sufficiency in the production of feeds and forages. Notwithstanding the above, the grasslands and pastureland in the country are continuously shrinking. The paper addresses important issues limiting the development of new grasslands in semiarid Bundelkhand region of Uttar Pradesh particularly the Lalitpur district

Expression profiling of genes regulated by TGF-beta: Differential regulation in normal and tumour cells

BACKGROUND: TGF-beta is one of the key cytokines implicated in various disease processes including cancer. TGF-beta inhibits growth and promotes apoptosis in normal epithelial cells and in contrast, acts as a pro-tumour cytokine by promoting tumour angiogenesis, immune-escape and metastasis. It is not clear if various actions of TGF-beta on normal and tumour cells are due to differential gene regulations. Hence we studied the regulation of gene expression by TGF-beta in normal and cancer cells. RESULTS: Using human 19 K cDNA microarrays, we show that 1757 genes are exclusively regulated by TGF-beta in A549 cells in contrast to 733 genes exclusively regulated in HPL1D cells. In addition, 267 genes are commonly regulated in both the cell-lines. Semi-quantitative and real-time qRT-PCR analysis of some genes agrees with the microarray data. In order to identify the signalling pathways that influence TGF-beta mediated gene regulation, we used specific inhibitors of p38 MAP kinase, ERK kinase, JNK kinase and integrin signalling pathways. The data suggest that regulation of majority of the selected genes is dependent on at least one of these pathways and this dependence is cell-type specific. Interestingly, an integrin pathway inhibitor, RGD peptide, significantly affected TGF-beta regulation of Thrombospondin 1 in A549 cells. CONCLUSION: These data suggest major differences with respect to TGF-beta mediated gene regulation in normal and transformed cells and significant role of non-canonical TGF-beta pathways in the regulation of many genes by TGF-beta

Nutrient Intake and Utilization in Jalauni Lambs Fed \u3cem\u3e Azolla \u3c/em\u3e Meal Supplemented Diet

Azolla is an important aquatic fungi due to the occurrence of both photosynthesis and nitrogen fixation in the leaves and also because of its profuse growth habbit, it appears as a potential source of protein, minerals and vitamins for livestock feeding. Keeping in view of the increasing cost and heavy deficit of concentrate ingredients in the country, an attempt was made to replace mustard cake protein @ 25% and 50% levels with Azolla (Microphylla) meal protein in the ration of sheep to investigate the effect of supplementation of azolla meal on nutrient intake and utilization in growing Jalauni lambs fed green chaffed MP Chari based rations

Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

Multiple enzymes are required for efficient hydrolysis of lignocellulosic biomass and no wild type organism is capable of producing all enzymes in desired levels. In this study, steam explosion of wheat straw was carried out at pilot scale and a synthetic enzyme mixture (EnzMix) was developed by partially replacing the cellulase with critical dosages of commercially available accessory enzymes (β-glucosidase, xylanase and laccase) through central composite design. Highest degree of synergism (DS) was observed with β-glucosidase (1.68) followed by xylanase (1.36). Finally, benchmarking of EnzMix (Celluclast, β-glucosidase and xylanase in a protein ratio of 20.40: 38.43: 41.16, respectively) and other leading commercial enzymes was carried out. Interestingly, hydrolysis improved by 75% at 6 h and 30% at 24 h, respectively in comparison of control. By this approach, 25% reduction in enzyme dosage was observed for obtaining the same hydrolysis yield with opitimized enzyme cocktail. Thus, development of enzyme cocktail is an effective and sustainable approach for high hydrolysis efficiency

Influence of cysteamine supplementation during in vitro culture of early stage caprine embryos on blastocyst production

The objective of this study was to evaluate the effect of cysteamine supplementation on embryo development in mCR2 media. The COCs (1251) were matured in TCM–199 medium containing FSH (5µg/ml), LH (10µg/ml), follicular fluid (10%), FBS (10%) with 3 mg/ml BSA for 27h at 38.5°C and 5% CO2 in an incubator. Matured oocytes were co-cultured with 1×106 spermatozoa/ml collected from a Sirohi buck in fertTALP (10% FBS+ 4mg/ml BSA and 10 µg/ml heparin) for 18 h in incubation. After 18 h of sperm-oocytes, co-incubation of oocytes with sperms were washed in embryo development medium to remove sperm cells adhered to zonapellucida. Presumptive zygotes (1,171) were selected and randomly divided into 2 groups. Group 1, the presumptive zygotes (610) were cultured in mCR2aa as a control for 12 days. Group 2, the presumptive zygotes (561) were culture in mCR2aa medium supplemented with 100 µM cysteamine for 10 days. The percentage of cleavage, morula, and blastocyst production in groups 1 and 2 were 36.39% and 31.71% (cleavage); 21.62% and 30.89% (morula); 4.95% and 8.98% (blastocyst) respectively. In conclusion, results indicated that the addition of cysteamine to the IVC medium stimulates caprine embryo development and blastocyst production