Expression of the <i>Pina</i> and <i>Pinb</i> genes in developing seeds of several wheat genotypes.

<p>a,b gene expression data at 14 and 30 days post anthesis (dpa), respectively; RPKM, reads per kilo base per million mapped reads. Details in brackets after genotype names on the X-axis indicates grain hardness index and endosperm texture of genotypes classified as hard (H) or soft (S) wheats. Genotypes with HI above 50 were classified as Hard. Wheat genotypes in each group are arranged left to right with respect to increasing hardness index. Boxes on the top indicate <i>Pin</i> alleles present in the genotypes. cDNA prepared from RNA extracted from developing wheat seeds at 14 DPA, was subjected to next generation sequencing. RNA-seq analysis was undertaken using CLC Genomic Workbench V8 to determine gene expression of <i>Pina</i> and <i>Pinb</i>. The star symbol indicates data not available.</p

Expression of the <i>Pinb-2</i>A, -2B, -2D genes in developing seeds of several wheat genotypes.

<p>a, b gene expression data at 14 and 30 days post anthesis (dpa) respectively; RPKM, reads per kilo base per million mapped reads. Boxes on the top indicate <i>Pin</i> alleles present in the genotypes. Details in brackets after genotype names on the X-axis indicates grain hardness index and endosperm texture of genotypes classified as hard (H) or soft (S) wheats. The asterisk indicates data not available.</p

Top down-regulated and up-regulated differentially expressed genes identified in HPAM (3a) and HPBM (3b) when compared to HNM group.

<p>Top down-regulated and up-regulated differentially expressed genes identified in HPAM (3a) and HPBM (3b) when compared to HNM group.</p

Total expression of <i>Pin</i> genes (<i>Pina</i> + <i>Pinb</i>) in developing wheat seeds of several wheat genotypes at 14- and 30-days post anthesis.

<p>Details in brackets after genotype names on the X-axis indicates grain hardness index and endosperm texture of genotypes classified as hard (H) or soft (S) wheats. Grain hardness was measured by SKCS analysis and genotypes with HI above 50 were labelled as hard. Details in brackets after genotype names on the X-axis indicates grain hardness index and endosperm texture of genotypes classified as hard (H) or soft (S) wheats. Boxes on the top indicate <i>Pin</i> alleles present in the genotypes. Transcriptome analysis by next generation sequencing data was undertaken to determine total <i>Pin</i> gene expression which was then normalised and expressed as reads per kilo base per million mapped reads (RPKM). The asterisk indicates data not available.</p

Grain hardness Index (HI) based on presence of Pin alleles genotypes were grouped into three different groups.

<p>SD—Standard deviation.</p

Differentially expressed genes identified in the hard wheats when compared to the soft wheats.

<p>2a. Soft vs HNM+HPAM+HPBM, 2b. Soft vs HPAM, 2c. Soft vs HPBM, 2d Soft vs HNM.</p

Expression of the <i>Pina</i> and <i>Pinb</i> genes in developing seeds of several wheat genotypes.

<p>a,b gene expression data at 14 and 30 days post anthesis (dpa), respectively; RPKM, reads per kilo base per million mapped reads. Details in brackets after genotype names on the X-axis indicates grain hardness index and endosperm texture of genotypes classified as hard (H) or soft (S) wheats. Genotypes with HI above 50 were classified as Hard. Wheat genotypes in each group are arranged left to right with respect to increasing hardness index. Boxes on the top indicate <i>Pin</i> alleles present in the genotypes. cDNA prepared from RNA extracted from developing wheat seeds at 14 DPA, was subjected to next generation sequencing. RNA-seq analysis was undertaken using CLC Genomic Workbench V8 to determine gene expression of <i>Pina</i> and <i>Pinb</i>. The star symbol indicates data not available.</p