Synthesis of Silver Nanoparticles by a Bryophilous Rhizoctonia species

We demonstrate the synthesis of silver nanoparticles by a potentially benign species of bryophilous Rhizoctonia in two different media. The first medium supports fungal growth and the up‐regulation of nitrate reductase, while the second medium supports fungal growth and the repression of nitrate reductase. For both media, the resulting silver nanoparticles were ca. 25‐50nm and were subglobose to broadly ellipsoidal in shape. The optical analysis of the silver nanoparticles from both media demonstrated plasmon resonance at 415nm, confirming their metallic properties. The liquid colour change typically observed for extracellular silver nanoparticle formation was absent in both media. The silver nanoparticles in the two different media displayed different chemical associations; fewer associated chemicals were found with the media, which supported the up‐regulation of nitrate reductase. Another difference included plate‐like silver nanoparticle conglomerations, which were only encountered on hyphae from the medium that repressed nitrate reductase. There was also a noticeable difference in the capping agent formations between each media. The Rhizoctonia isolate examined in this study is suitable for large‐scale industrial applications because it does not produce spores and would have minimal impact on air quality

Preliminary Study of Cave Sample Storage Conditions on Fungal Community Diversity

We investigated the effect of varying storage time and storage temperature on fungal species’ isolation as part of a case study of Illinois cave sediment samples. A deeper understanding of cave fungal communities may influence eco-epidemiology studies of emerging or re-emerging cave fungal pathogens. Using culture-dependent techniques, we isolated geophilic fungi from homogeneous cave sediment samples from three Illinois caves. Each sample was stored under five different temperatures ranging from −80 °C to 22 °C. Cave sediment was periodically removed at five different time periods from 48 h to 1 year, serially diluted with distilled water, lawn plated onto two different media, and monitored for fungal colonies. We isolated colonies and confirmed identity through nrDNA sequence similarity. Our results suggest that storage time was more important than storage temperature for the isolation of a wide diversity of geophilic fungal taxa. Importantly, our results show that varying storage conditions will alter both the kind of taxa and abundance of those taxa, suggesting that comparative studies of fungal diversity across studies should employ similar storage conditions. Lastly, future investigations should utilize multiple genetic markers because the fungal barcode region lacked species-level resolution for many isolates within common Illinois geophilic fungal genera

Nutritional capability of and substrate suitability for Pseudogymnoascus destructans, the causal agent of bat white-nose syndrome.

Pseudogymnoascus destructans, the causal agent of bat white-nose syndrome, has caused nearly six million deaths in North American bats since its introduction into the United States in 2006. Current research has shown that caves can harbor P. destructans even after the infected bats are removed and bats no longer visit or inhabit previously infected caves. Our research focuses on elucidating reservoir requirements by investigating the nutritional capabilities of and substrate suitability requirements for six different P. destructans isolates from various localities including Illinois, Indiana, New York (Type specimen), and Pennsylvania. Enzyme assays implicate that both urease and b-glucosidase appear to be constitutive, lipase and esterase activity were more rapid than proteinase activity on 6% gelatin, gelatin degradation was accompanied by medium alkalinization, the reduction of thiosulfate generated hydrogen sulfide gas, chitinase and manganese dependent peroxidase activity were not visually demonstrated within eight weeks, and keratinase activity was not evident at pH 8 within eight weeks. We demonstrate that all P. destructans isolates are capable of growth and sporulation on dead fish, insect, and mushroom tissues. Sole nitrogen source assays demonstrated that all P. destructans isolates exhibit Class 2 nitrogen utilization and that growth-dependent interactions occur among different pH and nitrogen sources. Substrate suitability assays demonstrated that all isolates could grow and sporulate on media ranging from pH 5-11 and tolerated media supplemented with 2000 mg/L of calcium and 700 mg/L of three separated sulfur compounds: thiosulfate L-cysteine, and sulfite. All isolates were intolerant to PEG-induced matric potential with delayed germination and growth at -2.5 MPa with no visible germination at -5 MPa. Interestingly, decreasing the surface tension with Tween 80 permitted germination and growth of P. destructans in -5 MPa PEG medium within 14 days suggesting a link between substrate suitability and aqueous surface tension altering substances

A Survey of Bacteria and Fungi Associated with Leaves, Rhizophylls, and Vesicles of the Carnivorous Plant <i>Genlisea hispidula</i> (Lentibulariaceae)

Carnivorous plants capture, digest, and absorb prey via specialized structures such as bladders, pitchers, and other modified leaf traps. Studies have shown that not all carnivorous plants produce digestive enzymes; instead, some species rely on microbes living within their traps to produce the necessary enzymes required for prey digestion. Therefore, this study investigated the microbial community (bacteria and fungi) associated with Genlisea hispidula, a rare carnivorous species. Photosynthetic leaves, rhizophylls, and vesicles were processed after either being cleaned and rinsed in sterile water or after being surface sterilized. Tissues were ground in sterile water, serially diluted, lawn plated onto potato dextrose agar, and incubated in darkness for 24 h at 18–23 °C. Axenic cultures were obtained. Identity was determined via molecular sequence similarity of the full bacterial 16S rDNA gene or fungal ITS barcode regions. In total, 48 bacterial species and 29 fungal species were isolated, with Acidocella facilis and Burkholderia spp. being the most dominant isolated bacteria, and Trichomonascus vanleenenianus and Saitozyma spp. being the most dominant isolated fungi. Microbial diversity was greatest on photosynthetic leaves, while the vesicles had the lowest microbial diversity. This study is important because microbial communities play vital roles in maintaining host health and may be required when considering conservation

Nutritional Capability of and Substrate Suitability for Pseudogymnoascus destructans, the Causal Agent of Bat White-Nose Syndrome

Pseudogymnoascus destructans, the causal agent of bat white-nose syndrome, has caused nearly six million deaths in North American bats since its introduction into the United States in 2006. Current research has shown that caves can harbor P. destructans even after the infected bats are removed and bats no longer visit or inhabit previously infected caves. Our research focuses on elucidating reservoir requirements by investigating the nutritional capabilities of and substrate suitability requirements for six different P. destructans isolates from various localities including Illinois, Indiana, New York (Type specimen), and Pennsylvania. Enzyme assays implicate that both urease and b-glucosidase appear to be constitutive, lipase and esterase activity were more rapid than proteinase activity on 6% gelatin, gelatin degradation was accompanied by medium alkalinization, the reduction of thiosulfate generated hydrogen sulfide gas, chitinase and manganese dependent peroxidase activity were not visually demonstrated within eight weeks, and keratinase activity was not evident at pH 8 within eight weeks. We demonstrate that all P. destructans isolates are capable of growth and sporulation on dead fish, insect, and mushroom tissues. Sole nitrogen source assays demonstrated that all P. destructans isolates exhibit Class 2 nitrogen utilization and that growth-dependent interactions occur among different pH and nitrogen sources. Substrate suitability assays demonstrated that all isolates could grow and sporulate on media ranging from pH 5–11 and tolerated media supplemented with 2000 mg/L of calcium and 700 mg/L of three separated sulfur compounds: thiosulfate L-cysteine, and sulfite. All isolates were intolerant to PEG-induced matric potential with delayed germination and growth at −2.5 MPa with no visible germination at −5 MPa. Interestingly, decreasing the surface tension with Tween 80 permitted germination and growth of P. destructans in −5 MPa PEG medium within 14 days suggesting a link between substrate suitability and a

In Vitro Observations of the Interactions between Pholiota carbonaria and Polytrichum commune and Its Potential Environmental Relevance

Wildfires play a critical role in maintaining biodiversity and shaping ecosystem structure in fire-prone regions, and successional patterns involving numerous plant and fungal species in post-fire events have been elucidated. Evidence is growing to support the idea that some post-fire fungi can form endophytic/endolichenic relationships with plants and lichens. However, no direct observations of fire-associated fungal–moss interactions have been visualized to date. Therefore, physical interactions between a post-fire fungus, Pholiota carbonaria, and a moss, Polytrichum commune, were visually examined under laboratory conditions. Fungal appressoria were visualized on germinating spores and living protonemata within two weeks of inoculation in most growth chambers. Appressoria were pigmented, reddish gold to braun, and with a penetration peg. Pigmented, reddish gold to braun fungal hyphae were associated with living tissue, and numerous mature rhizoids contained fungal hyphae at six months. Inter-rhizoidal hyphae were pigmented and reddish gold to braun, but no structures were visualized on mature gametophyte leaf or stem tissues. Based on our visual evidence and previous work, we provide additional support for P. carbonaria having multiple strategies in how it obtains nutrients from the environment, and provide the first visual documentation of these structures in vitro

Effect of competitive terminal electron acceptor processes on dechlorination of cis-1,2-dichloroethene and 1,2-dichloroethane in constructed wetland soils

Thermodynamic calculations were coupled with time-series measurements of chemical species (parent and daughter chlorinated solvents, H(2), sulfite, sulfate and methane) to predict the anaerobic transformation of cis-1,2-dichloroethene (cis-1,2-DCE) and 1,2-dichloroethane (1,2-DCA) in constructed wetland soil microcosms inoculated with a dehalorespiring culture. For cis-1,2-DCE, dechlorination occurred simultaneously with sulfite and sulfate reduction but competitive exclusion of methanogenesis was observed due to the rapid H(2) drawdown by the dehalorespiring bacteria. Rates of cis-1,2-DCE dechlorination decreased proportionally to the free energy yield of the competing electron acceptor and proportionally to the rate of H(2) drawdown, suggesting that H(2) competition between dehalorespirers and other populations was occurring, affecting the dechlorination rate. For 1,2-DCA, dechlorination occurred simultaneously with methanogenesis and sulfate reduction but occurred only after sulfite was completely depleted. Rates of 1,2-DCA dechlorination were unaffected by the presence of competing electron-accepting processes. The absence of a low H(2) threshold suggests that 1,2-DCA dechlorination is a cometabolic transformation, occurring at a higher H(2) threshold, despite the high free energy yields available for dehalorespiration of 1,2-DCA. We demonstrate the utility of kinetic and thermodynamic calculations to understand the complex, H(2)-utilizing reactions occurring in the wetland bed and their effect on rates of dechlorination of priority pollutants

The oldest fossil mushroom.

A new fossil mushroom is described and illustrated from the Lower Cretaceous Crato Formation of northeast Brazil. Gondwanagaricites magnificus gen. et sp. nov. is remarkable for its exceptional preservation as a mineralized replacement in laminated limestone, as all other fossil mushrooms are known from amber inclusions. Gondwanagaricites represents the oldest fossil mushroom to date and the first fossil mushroom from Gondwana

The frequency of the diplotypes found in hunter-harvest white-tailed deer samples from Jo Daviess, LaSalle, and Winnebago Counties, Illinois.

nd = not detected; past = 2007–2012; JD = Jo Daviess; LS = LaSalle; Win = Winnebago; total unique diplotypes = 67; Diplotypes based on Haley et al. 2021 primers; deer with the haplotypes AA are highly vulnerable to CWD; that deer with a CC or an FF are considered to have a lower vulnerability to CWD; that deer with an AC, AF are less vulnerable than AA alone; Total is the frequency of each diplotype in the entire sample pool; Frequencies are based on Haley primers. (DOCX)</p

The number of confirmed CWD cases per hunter harvest samples tested from all sources for fiscal years 2003 to 2020 in the Illinois counties examined.

The number of confirmed CWD cases per hunter harvest samples tested from all sources for fiscal years 2003 to 2020 in the Illinois counties examined.</p