3 research outputs found
Endophytic fungi associated with four endemic wild coffee species (Mascarocoffea) in Madagascar
Mascarocoffea are wild coffee plants endemic to Madagascar. These
plants produce diverse, often specific biomolecules that are not found
in cultivated coffee plants. Production of these compounds could be due
to interactions between the endophytes and the host plant. Few studies
have been carried out on the richness and diversity of microorganisms
associated with these coffee plants. The objective of this study was to
identify endophytic fungi isolated from the leaves of species of
Mascarocoffea by morphological and molecular methods. Fifteen taxa were
morphologically identified among the 30 isolated. These included
Phyllosticta sp., Colletotrichum sp., Daldinia sp., Diaporthe
sp., Cladosporium sp., Fusarium sp.01, Fusarium sp. 02, Fusarium
sp. 03, Monilinia sp., Trichoderma sp., Alternaria sp,
Penicillium sp., Aspergillus niger , Rhizopus sp. and Nigrospora
sp. The phylum Ascomycota was the most represented, with 14 taxa and 1
taxon (Rhizopus sp.) belonging to the phylum of Zygomycota. Molecular
characterisation confirmed of the identity of these 15 taxa and those
of the morphologically Unidentified (NI) mycotaxa including
Colletotrichum karstii , Colletotrichum siamense , Neofusicoccum
parvum , Colletotrichum siamense, Punctularia strigosozonata ,
Stemphylium solani , Phoma multirostrata , Calophoma complonata,
Daldinia vanderguchtiae , Phoma exigua and Boremia exigua . This
study allowed us to identify the endophytic fungi isolated from
Mascarocoffea leaves from Madagascar.Les Mascarocoffea sont des caf\ue9iers sauvages end\ue9miques de
Madagascar. Ces plantes produisent des biomol\ue9cules
diversifi\ue9es, souvent sp\ue9cifiques et inexistantes chez les
caf\ue9iers cultiv\ue9s. La production de ces compos\ue9s
pourrait \ueatre due aux interactions des microorganismes endophytes
et la plante h\uf4te. Peu d\u2019\ue9tude a \ue9t\ue9
r\ue9alis\ue9e sur la richesse et la diversit\ue9 des
microorganismes associ\ue9s \ue0 ces caf\ue9iers.
L\u2019objectif de cette \ue9tude est d\u2019identifier les
champignons endophytes isol\ue9s \ue0 partir des feuilles de quatre
esp\ue8ces de Mascarocoffea par la m\ue9thode morphologique et
mol\ue9culaire. Quinze taxons ont pu \ueatre identifi\ue9s
morphologiquement parmi les 30 isol\ue9s. Ce sont\ua0: Phyllosticta
sp., Colletotrichum sp., Daldinia sp., Diaporthe sp.,
Cladosporium sp., Fusarium sp.01, Fusarium sp.02, Fusarium sp. 03,
Monilinia sp., Trichoderma sp., Alternaria sp., Penicillium
sp., Aspergillus niger , Rhizopus sp. et Nigrospora sp. Le phylum
des Ascomycota est le mieux repr\ue9sent\ue9 avec 14 taxons et 1
taxon (Rhizopus sp.) appartient au phylum de Zygomycota. La
caract\ue9risation mol\ue9culaire a permis de confirmer
l\u2019identit\ue9 de ces 15 taxons et celles des mycotaxons
morphologiquement Non Identifi\ue9es (NI) dont Colletotrichum karstii
, Colletotrichum siamense , Neofusicoccum parvum , Colletotrichum
siamense, Punctularia strigosozonata , Stemphylium solani , Phoma
multirostrata , Calophoma complonata, Daldinia vanderguchtiae , Phoma
exigua et Boremia exigua . Cette \ue9tude nous a permis
d\u2019identifier les champignons endophytes des feuilles de
Mascarocoffea de Madagascar