Цитопротекторная активность феновина в митоген-активированных клетках Jurkat

Department of Anatomy, Tbilisi State Medical UniversityThe aim of the study: to establish the cytoprotective ability of the application of Phenovine to mitogen-activated Jurkat cells’ modeling systems. Experiments were conducted on intact and PHA-stimulated Jurkat cells and in conditions of coincubation of intact and PHA-stimulated cells. Cytoprotectiv activity of Phenovine was established according to coefficients of viability of the cells and apoptosis intensity, calculated on the basis of the data from the MTT test. Based on the analysis of experimental data, it was concluded that the viability of Jurkat cells decreases (apoptosis intensity increases), while the rising ratio of activated and nonactivated cells in the incubation medium ([Jurkat]PHA/[Jurkat]intact). Therefore, Phenovin increases viability of PHA-stimulated Jurkat cells and prevents their apoptosis.Целью работы явилось исследование цитопротекторной активности феновина на митоген-активированных клетках Jurkat. Эксперименты проводились на интактных, PHA-стимулированных клетках Jurkat и в условиях совместной инкубации интактных и стимулированных клеток. Цитопротекторная активность феновина определялась по значению коэффициентов жизнеспособности клеток и интенсивности их апоптоза, которые рассчитывались из данных МТТ теста. На основании анализа результатов исследования сделано заключеиие, что жизнеспособность клеток Jurkat уменьшается, интенсивность апоптоза увеличивается, с увеличением соотношения PHA-стимулированных и не стимулированных клеток Jurkat в среде инкубации ([Jurkat]PHA/[Jurkat]интактные). Феновин увеличивает жизнеспособность PHA-стимулированных клеток Jurkat и предотвращает апоптоз

Some peculiarities of lipid and hormonal metabolism and mechanical properties of the blood in menopausal women

Department of Medical Physics and Biophysics, Department of Human Normal Anatomy, State Medical University of Tbilisi, GeorgiaBackground: Many aspects of age-related correlation and causal relationship between the mechanical properties of red blood cells (RBCs) and parameters of lipid and hormonal metabolism in women are unclear till now. Material and methods: 58 reproductive-aged (less than 45 years) and 32 menopausal (more than 45 years) women admitted to the Central Clinic of Tbilisi State Medical University, Georgia were investigated. In each group we studied blood estradiole, free nitric oxide (NO) content, density of inactive form of β2-adrenergic receptors (β2ARs) and mechanical property of RBCs membrane. Results: In the blood of menopausal women NO content and deviation of volume (DV) of RBCs, reflecting their deformability, with lower density of inactivated β2ARs on the surface of RBCs membranes was greater than the corresponding parameter in the reproductive-aged women. DV of RBCs rapidly decreased at low HDL (high-density lipoprotein) in reproductive-aged women and wasn’t sensitive to HDL content in menopausal women. Conclusions: Lack of blood estrogens content in menopausal women causes the development of dislipidemia, impairment of renewal of phospholipids in RBCs membranes, decreased activity of adrenergic structures and the subsequent decrease in the NO production. These factors contribute to reducing deformability of RBCs membrane and impairment of mechanical properties of the blood

Prevalence And Prognosis Of Candidiasis Among Covid-19 Patients: Data From ICU Department

Yeast species belonging to the Candida genus, including Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, and Candida krusei, are the most prevalent fungal species inhabiting various mucosal surfaces, such as the skin and the respiratory, digestive, and urinary tracts [1]. Although being commensal within the human host, Candida species are equipped with virulence attributes, enabling them to invade when opportunities arise and cause various infections in humans, especially when the immune system is impaired. We retrospectively studied total lymphocytes count and   Candida infection in 142 patients   who were admitted to TSMU The First university Clinic Intensive care unit department between 14.10.20 – 02.02.21 . Among  142 patients Candida  spp  were isolated in 15,4%(22/142) patients. In all this patients  total lymphocytes count were low.  Candidemia was  not dectected.  Candiduria was observed in 2 patients(9%, 2/22). Our data is close to the  White, P.L. et al studies from UK which have reported 12.6%(17/135) of Candidiasis

Several Data of Infection in COVID-19 Patients during March-June 2020 at the First University Clinic of Tbilisi State Medical University, Georgia

The emergence of disease COVID-19 in China at the end of 2019 is a major public health issue. Public health and infection control measures are urgently required to limit the spread of this global viral infection.160 patients were admitted to the First University Clinic of Tbilisi State Medical University (TSMU) during March-June 2020. We  retrospectively studied these cases according to gender and age of patients, lethal outcome rate, hospitalization days and  frequency of different comorbid diseases. Also, observations were carried out on patients taking angiotensin converting enzyme(ACE) inhibitors and angiotensin 1(AT1) receptor blockers due to heart failure or arterial hypertension. Among COVID-19 lethal outcome patients, frequency between female and male was not significantly different (44.4% vs. 55.6%). Frequency of infected patients was the most common in 30-59 of ages (45.3%). Average hospitalization days were 21. Mortality rate was 5.6%, mostly in age above 60 (88.8%). Among lethal outcome patients, 88.8% had comorbid diseases. Heart failure, acute kidney injury, sepsis, essential arterial hypertension III, mitral valve defect, nontoxic diffuse goiter and etc; were the most common underlying conditions. Covid-19 Convalescent patients with arterial hypertension (55%) which were not treated with angiotensin converting enzyme inhibitors and angiotensin 1 receptor blockers, required less hospitalization days, approximately 2-3 days (62 hours)

Comorbidity and Association of Posttraumatic Stress, Depression, Anxiety, and Somatic Complaints in COVID-19 Georgian Patients at the Beginning of Pandemic

Background: The global pandemic which the world has been facing for the past two years has demonstrated the need to study the effects of this virus on mental health. Various studies showed that COVID-19 could be a threat to people's mental health and physical health, yet the findings are still very limited. The purpose of the study was to fill an existing gap in the corresponding literature by analyzing Post Traumatic Stress Disorder (PTSD) symptoms, somatic complaints, depression, and anxiety in COVID-19 patients and studying their comorbidity to determine the impact of the virus on the patients' mental well- being.Methods: Patients diagnosed with COVID-19 took part in the study one month after their discharge from the hospital, accounting for 10% of all COVID-19 patients across Georgia during the research. PTSD Checklist (PCL-5) was used. Depression, somatic symptoms, and anxiety were assessed using the Patient Health Questionnaire (PHQ).Results: The results have shown that COVID-19, as a traumatic event, presents an association with PTSD, depression, anxiety, and somatic complaints. A high prevalence of depression (38,6%), anxiety (34,9%), and somatic symptoms (47%) was displayed. The overall indirect effect of PTSD on somatic symptoms was significant through depression and anxiety: 0.16, 95% CI [0.08, 0.26]. According to the report, the indirect effect of PTSD on somatic symptoms of depression was 0.12, 95% CI [0.05, 0.20].Conclusion: The study presents important findings on the relations between COVID-19 and patients mental health. Somatic complaints, depression, anxiety, and PTSD symptoms were prevalent in participants even after a month since they had COVID-19. Correlations between somatic complaints, anxiety, depression, and PTSD were also demonstrated. Even though there are various limitations to this study, how COVID-19 could affect mental health warrants further, more detailed research, which is necessary.Stress-related psychiatric disorders across the life spa

Large Hematoma Of Rectus Abdominis Muscle As A Rare Surgical Case In A COVID-19 Patient

Presented article discusses the case of spontaneous   large hematoma of rectus abdominis muscle, developed in a 63-year-old woman during standard in-hospital treatment of COVID-19.Diagnosis has been confirmed on the basis of clinical, ultrasound and computed tomography findings. Patient underwent conservative treatment, but due to the increase in size of hematoma and patient’s worsened general condition, emergency surgical hematoma evacuation and ligation of damaged lower epigastric artery was performed.Postoperative period passed without complications. Patient discharged at home under ambulatory follow-up by surgeon.According to this case could be concluded that conservative treatment of rectus abdominis muscle large hematoma is not always successful and careful monitoring and proper surgical treatment is needed to prevent complication and save the patient’s life

COVID-19 Vaccination and Pregnancy: Single Hospital Case Report

Reported case has been taken under consideration because of its close relation to the fact of vaccination against COVID-19 (Pfizer BionTech)  first dose.Multiparous woman at age of 40 years with normal progressive 15 weeks of gestation, has been admitted to the University Clinic on the 10-th day after COVID -19 vaccination with general weakness, joint pain, severe to moderate head ache, fever, at the admission COVID-19 PCR testing occurs to be positive.Following the protocol of COVID-19 pregnancy management anticoagulant treatment has been started (D-dimer 0.9 mg/dl). The   patient were under observation of infectious disease specialist.  Almost all other protocol based test results within normal ranges, on the 14-th day of vaccination gingival bleeding of moderate severity has been started together with progressive thrombocytopenia (63-103 m/L later 57-103 m/L), test of thromb agglutination was positive and the diagnose of idiopathic thrombocytopenia has been announced by hematologist.  Dexamethasone treatment has been initiated with following progressive positive results of thrombocyte count.  On the 12-th day of the admission with normal clinical and laboratory data’s patient has been discharged from the hospital with biweekly follow up for 8 weeks. All follow up laboratory values and clinical data were normal during this period. Based on literature, vaccination induced thrombocytopenia is a rare complication although has been documented and only several of them has proven to be vaccination induced complication. The reported case after assessment of patient’s history, clinical, lab data and VITT adapted scoring system revealed intermediate probability of VITT, coincidence of COVID-19 made difficulties for differentiation of thrombocytopenia reason which needs more accurate laboratory assessment, but unremarkable medical history and short time between vaccination and clinically revealed thrombocytopenia highly suspicious for VITT

COVID-19 Pregnancy and Psycho-neurological Disturbance: Single Hospital Case Report

31 years old pregnant woman at 38 1/7 week of gestation with fever has been admitted to the TSMU   First University Clinic Emergency, with positive COVID-19 PCR test. With characteristic complains of COVID-19 infection.  Family history not significant. Personal history reveals childhood seizure as a result of fever. Vital signs at the admission within normal ranges. All protocol based laboratory tests has been done and protocol based treatment initiated. On the seventh day of Covid-19 infection because of episodes of desaturation and termed gestation, pregnancy termination by induction has been done successfully. At the end of early puerperal period because of hypoxemia resistant to oxygen therapy and CT scan diagnosed severe viral induced pneumonia, with symptoms of encephalopathy has been documented.  Later patient transferred to the mechanical ventilation, protocol based lab tests, diagnostic procedures and treatment initiated. After 1 month with improved condition patient has been transferred to the ob/gyn department for ongoing treatment and rehabilitation at this time severely expressed symptoms of encephalopathy were documented.The underlying mechanisms of neurologic complications in patients with COVID-19 are diverse and, in some cases, multifactorial. Neurologic complications may arise from direct effects of the virus as well as systemic response to the infection or as a result of long lasting inadequate oxygenation of all tissues. Although mechanical ventilation is highly complicated by brain damage, covid-19 induced encephalopathies are as well documented and need more scientifically proved facts of the viral role in this complication