Effect of similar feeding regime on growth and body composition of Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) under mono and polyculture

Growth performance and body composition of yearling Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) was evaluated in semi-intensive (mono and polyculture) systems for 90 days. Prior to stocking, all ponds were fertilized with organic and inorganic manures. This application was repeated every two weeks throughout the study period. Supplementary feed containing 35% protein was applied daily at 3% of wet body weight. In trial 1, all the three species gained significantly higher weights with experimental feed (F1) versus control group (F0). There was non-significant difference observed among species. In trial 2, non-significant difference was observed for net weight gain among species and between feeds. The feed conversion ratio (FCR), protein efficiency ratio (PER), protein utilization (PU), gross nitrogen retention efficiency (GNRE%) and gross energy retention efficiency (GERE%) were found non-significantly different among species in both trials, except GNRE% in polyculture, where L. rohita showed significantly higher values than its counterparts. No significant difference was observed in body composition and mineral contents among species and between feeds in both trials. In conclusion, all the three fish species performed well under monoculture system with 35% protein diet and showed significantly higher growth than the control, compared to polyculture, without any significant effect on body composition.Keywords: Artificial feed, Indian major carps, growth, proximate composition, nutrient retention efficiency, cost effectivenes

Emergence of resistance to fluoroquinolones and third-generation cephalosporins in Salmonella Typhi in Lahore, Pakistan

Extensively drug-resistant (XDR) Salmonella Typhi has been reported in Sindh province of Pakistan since 2016. The potential for further spread is of serious concern as remaining treatment options are severely limited. We report the phenotypic and genotypic characterization of 27 XDR S. Typhi isolated from patients attending Jinnah Hospital, Lahore, Pakistan. Isolates were identified by biochemical profiling; antimicrobial susceptibility was determined by a modified Kirby-Bauer method. These findings were confirmed using Illumina whole genome nucleotide sequence data. All sequences were compared to the outbreak strain from Southern Pakistan and typed using the S. Typhi genotyping scheme. All isolates were confirmed by a sequence analysis to harbor an IncY plasmid and the CTX-M-15 ceftriaxone resistance determinant. All isolates were of the same genotypic background as the outbreak strain from Sindh province. We report the first emergence of XDR S. Typhi in Punjab province of Pakistan confirmed by whole genome sequencing

Trends in antimicrobial resistance amongst pathogens isolated from blood and cerebrospinal fluid cultures in Pakistan (2011-2015): A retrospective cross-sectional study

While antimicrobial resistance (AMR) continues to be a major public health problem in Pakistan, data regarding trends of resistance among pathogenic bacteria remains scarce, with few studies presenting long-term trends in AMR. This study was therefore designed to analyze long-term AMR trends at a national level in Pakistan. We report here results of a comprehensive analysis of resistance, among pathogens isolated from blood and cerebrospinal fluid (CSF), between 2011 and 2015. Susceptibility data was obtained from a local laboratory with collection points all across Pakistan (Chughtai Laboratory). Resistance proportions to most commonly used antimicrobials were calculated for each pathogen over a period of five years. While Acinetobacter species demonstrated highest resistance rates to all tested antimicrobials, a sharp increase in carbapenem resistance was the most noticeable (50%-95%) between 2011-2015. Our results also highlight the presence of third and fourth generation cephalosporins resistance in Salmonella enterica serovar Typhi in Pakistan. Interestingly, where rise in AMR was being observed in some major invasive pathogens, decreasing resistance trends were observed in Staphylococcus aureus, against commonly used antimicrobials. Overall pathogens isolated from blood and CSF between 2011-2015, showed an increase in resistance towards commonly used antimicrobials

Effect of Plant-Fishmeal and Plant By-Product Based Feed on Growth, Body Composition and Organoleptic Flesh Qualities of Labeo rohita

Abstract.-Present study was aimed at evaluating the effect of plant-fishmeal feed and/or plant by-product based feed on growth, body composition and flesh quality of Labeo rohita. Fish fed on rice polish alone served as control (T 0 ). Fish feed ingredients were grouped together with two ingredients in each test diet i.e. guar meal and canola meal (T 1 ), soybean meal and cotton seed meal (T 2 ), guar meal and cotton seed meal (T 3 ), soybean meal and canola meal (T 4 ) and fishmeal and canola meal (T 5 ). Each treatment including control had two replicates. Earthen ponds (12) with uniform area of 0.03 ha each, were randomly stocked with 100 fish (average weight 200 g) in each. All the ponds were then randomly allotted to individual treatment including control and were fed at 4% of their wet biomass twice a day. Significantly higher growth performance, specific growth rate (SGR) and better food conversion ratio (FCR) in T 3 and T 1 than rest of the treatments suggested that guar meal and cotton seed meal, and guar meal and canola meal are better option to include in future feed formulations for maximum performance and minimum feed wastage. Fish samples showed higher protein values in T 4 , fat in T 2 , moisture contents in control, dry matter in T 1 and ash in T 5. Nonsignificant differences in flavor, juiciness, and oiliness indicated that the sensory attributes of fish flesh were not affected by feeding fish with blend of various ingredients. Our study revealed that the cotton seed meal which is considered toxic to fish and is restricted in fish feeds perform equally good and can be incorporated in future feed formulations for Labeo rohita

RNAseq of diverse spring wheat cultivars released during last 110 years

Abstract Here, we performed RNA-seq based expression analysis of root and leaf tissues of a set of 24 historical spring wheat cultivars representing 110 years of temporal genetic variations. This huge 130 tissues RNAseq dataset was initially used to study expression pattern of 97 genes regulating root growth and development in wheat. Root system architecture (RSA) is an important target for breeding stress-resilient and high-yielding wheat cultivars under climatic fluctuations. However, root transcriptome analysis is usually obscured due to challenges in root research due to their below ground presence. We also validated the dataset by performing correlation analysis between expression of RSA related genes in roots and leaves with 25 root traits analyzed under varying moisture conditions and 10 yield-related traits. The Pearson’s correlation coefficients between root phenotypes and expression of root-specific genes varied from −0.72 to 0.78, and strong correlations with genes such as DRO1, TaMOR, ARF4, PIN1 was observed. The presented datasets have multiple uses such as a) studying the change in expression pattern of genes during time, b) differential expression of genes in two very important tissues of wheat i.e., leaf and roots, and c) studying customized expression of genes associated with important phenotypes in diverse wheat cultivars. The initial findings presented here provided key insights into understanding the transcriptomic basis of phenotypic variability of RSA in wheat cultivars