Cloud Forensics : Isolating Cloud Instance

Cloud computing has been the trending model for storing, accessing and modifying the data over the Internet in the recent years. Rising use of the cloud has generated a new concept related to the cloud which is cloud forensics. Cloud forensics can be defined as investigating for evidence over the cloud, so it can be viewed as a combination of both cloud computing and digital forensics. Many issues of applying forensics in the cloud have been addressed. Isolating the location of the incident has become an essential part of forensic process. This is done to ensure that evidence will not be modified or changed. Isolating an instant in the cloud computing has become even more challenging, due to the nature of the cloud environment. In the cloud, the same storage or virtual machine have been used by many users. Hence, the evidence is most likely will be overwritten and lost. The proposed solution in this paper is to isolate a cloud instance. This can be achieved by marking the instant that reside in the servers as "Under Investigation". To do so, cloud file system must be studied. One of the well-known file systems used in the cloud is Apache Hadoop Distributed File System (HDFS). Thus, in this paper the methodology used for isolating a cloud instance would be based on the HDFS architecture

Diagnostic impact of intracranial vessel wall MRI in 205 patients with ischemic stroke or TIA

BACKGROUND AND PURPOSE: Secondary prevention of ischemic stroke depends on determining the cause of the initial ischemic event, but standard investigations often fail to identify a cause or identify multiple potential causes. The purpose of this study was to characterize the impact of intracranial vessel wall MR imaging on the etiologic classification of ischemic stroke. MATERIALS AND METHODS: This was a single-center, retrospective study of 205 consecutive patients who were referred for vessel wall MR imaging to clarify the etiology of an ischemic stroke or TIA. An expert panel classified stroke etiology before and after incorporating vessel wall MR imaging results using a modified Trial of Org 10172 in Acute Stroke Treatment system. We measured the proportion of patients with an altered etiologic classification after vessel wall MR imaging. RESULTS: The median age was 56 years (interquartile range = 44–67 years), and 51% (106/205) of patients were men. Vessel wall MR imaging altered the etiologic classification in 55% (112/205) of patients. The proportion of patients classified as having intracranial arteriopathy not otherwise specified decreased from 31% to 4% (64/205 versus 9/205; P < .001) and the proportion classified as having intracranial atherosclerotic disease increased from 23% to 57% (48/205 versus 116/205; P < .001). Conventional work-up classification as intracranial arteriopathy not otherwise specified was an independent predictor of vessel wall MR imaging impact (OR = 8.9; 95% CI, 3.0–27.2). The time between symptom onset and vessel wall MR imaging was not a predictor of impact. CONCLUSIONS: When vessel wall MR imaging is performed to clarify the etiology of a stroke or TIA, it frequently alters the etiologic classification. This is important because the etiologic classification is the basis for therapeutic decision-making

Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration

HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are proximal to super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results provide evidence of the relevance of the spatial compartmentalization of the genome for HIV-1 integration, thus further strengthening the role of nuclear architecture in viral infection.This work was supported by German Center for Infection Research (DZIF) Thematic Translational Unit HIV-1 04.704 Infrastructural Measure to M.L. and by the Hector Grant M70 “HiPNose: HiV Positioning in the Nuclear Space” to M.L. and M.S. We acknowledge the financial support of the Spanish Ministry of Economy and Competitiveness (“Centro de Excelencia Severo Ochoa 2013–2017,” Plan Nacional BFU2012–37168), of the CERCA (Centres de Recerca de Catalunya) Programme/Generalitat de Catalunya, and of the European Research Council (Synergy Grant 609989). K.V. and M.K. are supported by the European Structural and Investment Funds grant for the Croatian National Centre of Research Excellence in Personalized Healthcare (contract #KK.01.1.1.01.0010), Croatian National Centre of Research Excellence for Data Science and Advanced Cooperative Systems (contract KK.01.1.1.01.0009), and Croatian Science Foundation (grant IP-2014–09–6400)