The EBV-Encoded Oncoprotein, LMP1, Recruits and Transforms Fibroblasts via an ERK-MAPK-Dependent Mechanism

open access articleLatent membrane protein 1 (LMP1), the major oncoprotein encoded by Epstein–Barr virus (EBV), is expressed at widely variable levels in undifferentiated nasopharyngeal carcinoma (NPC) biopsies, fueling intense debate in the field as to the importance of this oncogenic protein in disease pathogenesis. LMP1-positive NPCs are reportedly more aggressive, and in a similar vein, the presence of cancer-associated fibroblasts (CAFs) surrounding “nests” of tumour cells in NPC serve as indicators of poor prognosis. However, there is currently no evidence linking LMP1 expression and the presence of CAFs in NPC. In this study, we demonstrate the ability of LMP1 to recruit fibroblasts in vitro in an ERK-MAPK-dependent mechanism, along with enhanced viability, invasiveness and transformation to a myofibroblast-like phenotype. Taken together, these findings support a putative role for LMP1 in recruiting CAFs to the tumour microenvironment in NPC, ultimately contributing to metastatic disease

Agency, ‘good motherhood’ and ‘a load of mush’: Constructions of baby-led weaning in the press

In this age of ‘intensive motherhood’, new mothers are flooded with information on the best ways in which to raise their children. One of the key issues is infant feeding, in particular, the timing and method of weaning their children onto solid food. This paper examines a new approach called ‘baby-led weaning’ (BLW) in which the child feeds themselves instead of being spoon-fed, that came into popular parenting culture in recent years, considering the ways in which it is represented in National and International newspapers. The media search database Proquest International Newsstand, was searched for ‘baby-led weaning’, producing an eventual sample of 78 articles from a number of countries. The articles were subjected to a critical discursive psychological analysis. The key themes that emerged from the newspapers focused around two main areas; the infant as agentive in their eating behaviours; and, constructions of maternal identities and resisting ‘good motherhood’

The EBV-encoded Oncoprotein, LMP1, recruits and transforms fibroblasts via an ERK-MAPK-dependent mechanism

Latent membrane protein 1 (LMP1), the major oncoprotein encoded by Epstein–Barr virus (EBV), is expressed at widely variable levels in undifferentiated nasopharyngeal carcinoma (NPC) biopsies, fueling intense debate in the field as to the importance of this oncogenic protein in disease pathogenesis. LMP1-positive NPCs are reportedly more aggressive, and in a similar vein, the presence of cancer-associated fibroblasts (CAFs) surrounding “nests” of tumour cells in NPC serve as indicators of poor prognosis. However, there is currently no evidence linking LMP1 expression and the presence of CAFs in NPC. In this study, we demonstrate the ability of LMP1 to recruit fibroblasts in vitro in an ERK-MAPK-dependent mechanism, along with enhanced viability, invasiveness and transformation to a myofibroblast-like phenotype. Taken together, these findings support a putative role for LMP1 in recruiting CAFs to the tumour microenvironment in NPC, ultimately contributing to metastatic disease