Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts

Abstract Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts. Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts. The fibroblasts were cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test. Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected to propolis were more viable when compared to calcium hydroxide (P < 0.05). Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament

In vitro evaluation of the antibacterial effects of AH26 combined with two different antibiotics on Enterococcus faecalis

Introduction: To prevent growth of residual bacteria, it has been proposed that sealers and filling materials should have antibacterial properties. The aim of this study was to evaluate the antibacterial effects of AH26 sealer combined with two different antibiotics on Enterococcus faecalis. Materials and methods: In this in vitro study, the antimicrobial effects of amoxicillin and minocycline which were added separately to AH26 sealer were evaluated on Enterococcus faecalis. In group 1, 25% amoxicillin with AH26 sealer, in group 2, 25% minocycline with AH26 sealer, in group 3, pure AH26 sealer, and in group 4, distilled water were used. The samples were placed in agar plates inoculated with Enterococcus faecalis. All the plates were incubated for 7 days at 37°C under anaerobic condition, and zones of growth inhibition were measured in millimeters at 24-, 48-, 72- and 7-day intervals. Data were analyzed using 2-way repeated measures ANOVA and multiple comparisons were made by Tukey tests (α=0.05). Results: The results showed that adding antibiotics at 25% concentrations to AH26 sealer created significantly larger zones of growth inhibition compared to the sealer alone (p value < 0.05). In groups 1 and 2, there were significant differences in zones of growth inhibition with an increase in incubation period from 14 to 72 hours; however, there were no significant differences between 72- hour and 1-week intervals. The maximum antibacterial activity was observed in the amoxicillin group. Conclusion: Mixing AH26 sealer with amoxicillin and minocycline significantly increases the antibacterial property of the sealer against Enterococcus faecalis

Comparison of Endodontic Medicaments on Bond Strength of Fiber Post to Root Dentin Using Resin Cement.

STATEMENT OF THE PROBLEM Endodontic irrigants and medicaments may affect the bond strength of intracanal posts to root dentin. PURPOSE The aim of this study was to compare the effect of calcium hydroxide (Ca(OH)2) and 2% chlorhexidine gel (CHX) on bond strength of fiber post cemented with resin cement to root dentin. MATERIALS AND METHOD This in vitro experimental study was conducted on 36 mandibular premolars. Canals were prepared using the step back technique. After root canal irrigation, the teeth were divided into three groups of 12. Ca(OH)2 paste and CHX gel were used as intracanal medicaments in the first and second groups respectively. No intracanal medicament was used in the third group (control group). Access cavities were then sealed and the teeth were incubated for one week. The root canals were then filled using gutta percha and AH26 sealer and the teeth were incubated for 72 hours. Tooth crowns were then cut at the level of the cementoenamel junction and intracanal posts were placed. The teeth were mounted in auto-polymerizing acrylic resin, and incubated for one week .They were then sectioned into 1.5mm thick slices from their coronal surface using a fully automated cutting machine, and subjected to push-out test until failure. The load at debonding was recorded and data were analyzed using one-way ANOVA, post-hoc test and t-test. The coronal margin of the root was at the level of the surface of acrylic resin in the mold. RESULTS The mean bond strength was 4.45 MPa in the Ca(OH)2, 2.45 MPa in the CHX and 2.48 MPa in the control group. The difference in this regard was statistically significant among groups (p= 0.04). The Ca(OH)2 group had significant differences with the CHX and control groups (p= 0.03 and p= 0.02, respectively). The difference between the CHX and control groups was not significant (p= 0.974). CONCLUSION Based on the results, Ca(OH)2 increased the bond strength of fiber post to root dentin but 2% CHX had no effect on bond strength

Effect of triple antibiotic paste and calcium hydroxide as intracanal medicaments on bond strength of composite to dentin

Objective: Considering the use of triple antibiotic paste (TAP) for root canal treatment of open apex teeth, this study aimed to assess the effect of TAP and calcium hydroxide (CH) on bond strength of composite to dentin. Methods: This in-vitro study was conducted on 32 extracted human premolar teeth. After disinfection with 2% thymol solution, the enamel on the buccal surface of specimens was removed to expose a smooth dentin surface parallel to the long axis of the teeth with approximately 19mm2 surface areas. Specimens were divided into three groups of 11, 10 and 11 specimens. In group one, TAP, in group two CH and in group three, saline solution were applied to dentin surfaces for 14 days. After removal of medicaments, composite cylinders were bonded to the dentin surfaces using a bonding agent. Shear bond strength was measured in an Instron machine at a crosshead speed of 1mm/min. Data were analyzed using one-way ANOVA. Results: The highest mean bond strength belonged to the control group (14.4760 MPa) and the lowest belonged to the TAP group (11.5808 MPa). The mean bond strength in CH group was less than that of the control and higher than that of the TAP group (11.7834 MPa). However, the difference among the three groups was not statistically significant (p=0.327). Conclusion: Use of medicaments such as CH and TAP has no effect on bond strength of composite to dentin

Effect of triple antibiotic paste and calcium hydroxide as intracanal medicaments on bond strength of composite to dentin

Objective: Considering the use of triple antibiotic paste (TAP) for root canal treatment of open apex teeth, this study aimed to assess the effect of TAP and calcium hydroxide (CH) on bond strength of composite to dentin.Methods: This in-vitro study was conducted on 32 extracted human premolar teeth. After disinfection with 2% thymol solution, the enamel on the buccal surface of specimens was removed  to expose a smooth dentin surface parallel to the long axis of the teeth with approximately 19mm2 surface areas. Specimens were divided into three groups of 11, 10 and 11 specimens. In group one, TAP, in group two CH and in group three, saline solution were applied to dentin surfaces for 14 days. After removal of medicaments, composite cylinders were bonded to the dentin surfaces using a bonding agent. Shear bond strength was measured in an Instron machine at a crosshead speed of 1mm/min. Data were analyzed using one-way ANOVA.Results: The highest mean bond strength belonged to the control group (14.4760 MPa) and the lowest belonged to the TAP group (11.5808 MPa). The mean bond strength in CH group was less than that of the control and higher than that of the TAP group (11.7834 MPa). However, the difference among the three groups was not statistically significant (p=0.327).Conclusion: Use of medicaments such as CH and TAP has no effect on bond strength of composite to dentin

The comparison of antibacterial effect of propolis , sodium hypochlorite 5.25%, and chlorhexidine 2% as intracanal irrigants against enterococcus faecalis: an ex vivo study

Introduction: Debridement of root canal using appropriately safe and effective irrigants is the key factor for long-term success. Purpose of this study was to compare the antibacterial effect of propolis with 5.25% sodium hypochlorite, and 2% chlorhexidine against enterococcus faecalis. Materials &Methods: In this study, 36 single-canal roots were used. The crown was removed and instrumentation was prepared by step-back technique, then teeth were sterilized and contaminated with E. Faecalis, and divided into four groups with 9 cases: group1: Propolis, group2: 5.25% sodium hypochlorite, group3: 2% chlorhexidine and group4: controls. Irrigants were injected by a 27-gauge syringe and roots were incubated in 37°C for one week. Sampling was done and inoculated to tryptone soy broth media, after 24 hours the turbidity was measured. Samples were also cultured on agar plates, and colony-forming units were counted as CFU/ml. Data were analysed using the Mann-Whitney test. Results: The difference between propolis with mean value of 246.77 colonies and chlorhexidine with mean value of zero colonies, was significant (P=.002). Similarly, the difference between chlorhexidine and sodium hypochlorite with mean value 203.55 of colonies was significant and they had significant difference in turbidity (P=.002), too. No significant difference was observed between propolis and sodium hypochlorite with regard to the induced colonies (P=0.781) and their turbidity (P=0.495). Conclusion: It can be concluded that antibacterial activity of 2% chlorhexidine against E. faecalis is more obvious than propolis or 5.25% sodium hypochlorite. But antibacterial activity of propolis over 5.25% sodium hypochlorite or vice versa was not confirmed

Survey of Undergraduate Dentistry Students Awareness about Pulp and Periodontal Lesions in Fourteen Cities of Iran

Inflammation and infection can spread from the dental pulp to the periodontal tissue and from the periodontium to the pulp. Given the challenging nature of diagnosing endodontic-periodontal lesions and the importance of appropriate treatment to improve dental prognosis, this study was performed to evaluate the knowledge of dental students about the diagnosis of pulp and periodontal lesions.Materials & Methods:In this analytical observational study, a questionnaire was designed to assess knowledge on pulp and periapical lesions in three areas of theoretical knowledge, knowledge in diagnosis and knowledge in treatment in the second semester of the academic year 2021 and filled out by 400 dental students representing 15 cities of Iran. Data analysis was performed by Friedman, Wilcoxon and Mann-Whitney tests (α = 0.05).Results:Percentage of correct answer of dental students to the questions related to theoretical knowledge 65.70 ± 18.39, percentage of correct answer to the questions of students' diagnostic knowledge 50.75 ± 33.28, percentage of correct answer to the questions of treatment knowledge 52.13 ± 23.93 and the percentage of correct answers to the questions of knowledge in total was 56.68 ± 16.35, which is statistically significant in terms of knowledge in the three areas studied (p value < 0.001).Conclusion:In the present study, it was shown that the highest level of appropriate knowledge in students is in the field of theoretical knowledge of endodontic-periodontal lesions and the least level of appropriate knowledge was in the field of diagnosis of these diseases. The overall knowledge of students in this study about endodontic-periodontal lesions was shown to be moderat

Evaluation of Oral Health-Related Quality of Life after Implant Treatment versus Root Canal Treatment

Preserving the natural tooth by endodontic treatment or tooth extraction and implantation is an important dilemma that requires careful evaluation of the factors that affect the final treatment and patients' quality of life. The aim of this study was to evaluate the quality of life related to oral health after single tooth implant treatment in comparison with endodontic treatment.Materials & Methods:This study is a cross-sectional analytical study to evaluate the quality of life of 80 patients referred to Isfahan Azad Dental School in 1399-1400 in two groups (endo and implant) whose anterior teeth have been endodontically treated and had also a history of anterior single tooth implant with functional crown in a recent year, with using Oral health-related quality of life questionnaire (OHIP-12). The collected data were analyzed using Shapiro-Wilk test and independent T-test with an error level of 0.05% (p value < 0.05).Results:Mean patient's oral health-related quality of life after single tooth implant and after endodontic treatment was respectively 16.95 and 16.83 and all the studied parameters were shown to be above average. There was no significant difference between the type of treatment and patient's oral health-related quality of life (p value = 0.885). There was a significant difference between patient's gender and oral health-related quality of life in implant treatment (p value = 0.036).Conclusion:The oral health-related quality of life after single tooth implant treatment was comparable to endodontic treatment in patients and it was higher than average in both treatment

Comparison of Clinical and Radiographic Results of Single Tooth Implants against Endodontic Treatment with Post and Crown Repair

The choice between the two modes of treatment, endodontic treatment and single tooth implants are very different depending on the dentist, design, definition of successful outcomes, evaluation methods, and sample size. The aim of this study was to compare the clinical and radiographic results of single tooth implants versus endodontic treatment with post and crown restoration in patients referred to the School of Dentistry of Islamic Azad University of Isfahan.Materials & Methods:In this cross-sectional analytical study in the academicyear 2021, 90 (46 females and 44 males) patients after recording demographic information and medical status, during three different time intervals post treatment (under 3 years, 3 to 5 years and Over 5 years) were examined clinically and radiographically (periapical). Outcome of treatment was classified based on clinical and radiographic findings into three categories: successful, surviving and unsuccessful. Data were analyzed using Chi-squared test (α = 0.05).Results:In this study, 60% of women and 40%of men had endodontic treatment and 42.2% of women and 57.8% of men had single tooth implants. There was no significant difference between the treatment outcomes in the studied groups (p value > 0.521). There was no statistically significant relationship between gender and type of treatment as well as gender and treatment satisfaction (p value > 0.05).Conclusion:Considering the preservation of natural teeth, if possible, root canal treatment is the main treatment of choice, otherwise a single-tooth implant can be a good alternative to missing or extracted teeth in terms of successful outcome

Injection of lidocaine alone versus lidocaine plus dexmedetomidine in impacted third molar extraction surgery, a double-blind randomized control trial for postoperative pain evaluation

[[abstract]]Objectives: Administration of medications such as dexmedetomidine as a topical anesthetic has been suggested in the pain control in dentistry. This double-blind randomized control trial study evaluated postoperative pain and associated factors following impacted third molar extraction surgery. Lidocaine alone was taken as the control and lidocaine plus dexmedetomidine as the intervention. Materials and methods: Forty patients undergoing mandibular third molar extraction entered the study and were randomly allocated to the control and interventional groups. 0.15 ml of dexmedetomidine was added to each lidocaine cartridge and the drug concentration was adjusted to 15 μg for the intervention group while only lidocaine was used in the control group. A visual analog scale was used to measure and record pain levels at the end of the surgery and 6, 12, and 24 hours after the surgery and number of painkillers taken by the patients after the surgery was also recorded. Results: Pain scores of the intervention group decreased significantly during the surgery and also 6, 12, and 24 hours after the surgery compared to the control group. The pain score was correlated significantly with our intervention during the surgery and also 6 and 12 hours after that (all P value 0.05). Conclusion: In patients undergoing molar surgery, administration of a combination of dexmedetomidine and lidocaine is beneficial for the pain control. Clinical Relevance. Compared to the injection of lidocaine alone, combination of dexmedetomidine and lidocaine can be used for a better pain control in molar surgeries