Microencapsulation of Bacillus velezensis Using Alginate-Gum Polymers Enriched with TiO2 and SiO2 Nanoparticles

Bacillus bacteria are a group of plant growth stimulants that increase plant growth and resistance to plant pathogens by producing various metabolites. With their large surface area and small size, nanoparticles can be used in controlled-release formulations and increase the efficiency of the desired product. Encapsulation of biological agents in combination with nanoparticles can be an essential step in increasing the performance of these agents in adverse environmental conditions. In this study, which is the result of a collaboration between scientists from Italy and Iran, Bacillus velezensis was encapsulated in alginate combined with whey protein and zedo, mastic, and tragacanth gums in the presence of silica and titania nanoparticles to obtain two-layer and multilayer assemblies acting as novel, smart micro-encapsulation systems. The results of laboratory studies showed that the B. velezensis could produce protease, lipase, siderophore, auxin, and a dissolution of mineral phosphate. Scanning electron microscopy images (SEM) showed that the studied microcapsules were almost spherical. Moisture affinity, swelling, and efficiency of each microcapsule were examined. The results showed that the highest encapsulation efficiency (94.3%) was related to the multilayer formulation of alginate-whey protein-zedo. XRD and FTIR spectroscopy showed that the alginate, whey protein, and zedo were mixed properly and no incompatible composition occurred in the reaction. This study aimed to provide a suitable formulation of biofertilizers based on biodegradable compounds as an alternative to chemical fertilizers, which is low cost and very effective without harming humans and the environment

Rapid and Green Synthesis of some Benzothiazole-, Benzimidazole-and Benzoxazole-2-thiol Derivatives Using Copper Sulfate in Aqueous Media

In this study an easy, green, efficient and simple approach is reported for the synthesis of some benzothiazole-, benzimidazole-and benzoxazole-2-thiol derivatives. The proposed approach employs the reaction of corresponding aromatic amine with potassium isopropyl xanthate (Z11) in the presence of copper sulfate (CuSO 4 ) as a catalyst under conventional heating and ultrasonic irradiation. The advantages of this protocol are: using water and glycerol as green solvents, commercially available precursors, simple work-up, an inexpensive catalyst, high yield and short reaction time

Determining the Most Important Soil Properties Affecting the Yield of Saffron in the Ghayenat Area

Introduction: Saffron is one of the most important economic plants in the Khorasan province. Awareness of soil quality in agricultural lands is essential for the best management of lands and for obtaining maximum economic benefit. In general, plant growth is a function of environmental factors especially chemical and physical properties of soil (20). It has been demonstrated that there was a positive and high correlation between soil organic matter and saffron yield. Increasing the yield of saffron due to organic matter is probably due to soil nutrient, especially phosphorous and nitrogen and also improvement of soil physical quality (6, 28, 29). The yield of saffron in soils with high nitrogen as a result of vegetative growth is high (8). Shahandeh (6) found that most of the variation of saffron yield depends on soil properties. Due to the economic importance of saffron and the role of soil properties on saffron yield, this research was conducted to find the relationship between saffron yield and some soil physical and chemical properties, and to determine the contribution of soil properties that have the greatest impact on saffron yield in the Ghayenat area. Materials and Methods: This research was performed in 30 saffron fields (30 soil samples) of the Ghayenat area (longitude 59° 10΄ 10.37˝ - 59° 11΄ 38.41˝ and latitude 33° 43΄ 35.08˝ - 33΄ 44΄ 02.78˝), which is located in the Khrasan province of Iran. In this research, 21 soil properties were regarded as the total data set (TDS). Then the principal component analysis (PCA) was used to determine the most important soil properties affecting saffron yield as a minimum data set (MDS) and the stepwise regression to estimate saffron yield. To estimate the yield of saffron in stepwise regression method, saffron yield was considered as a dependent variable and soil physical and chemical properties were considered to be independent variables. Results and Discussion: According to the PCA method, among the 21 studied properties, 7 out of them including calcium, iron, zinc contents, sand, calcium carbonate equivalent percent, mean weight diameter of aggregates (MWD) and manganese (Mn) had the higher Eigenvalues. Therefore, the above properties were introduced as the most important soil properties in saffron fields. Calcium carbonate had the negative effect on the availability of micro-nutrients (26). Christensen et al. (15) found that by increasing the calcium carbonate in soil due to high pH and formation of insoluble components, the uptake of micro-nutrients is especially limited. The results of stepwise regression method (equation 1) showed that soil acidity (pH), zinc content, bulk density, MWD, iron content, salinity (EC), organic carbon and available potassium in soil were the most important properties that affect the yield of saffron, so that the determination coefficient (R2) of the regression model was high (Table 2) and it can explain 74% of the variation of saffron yield. Y = 6924.51 – 1187.31 pH – 89.65 EC + 71.6 Fe – 826.02 Zn + 471.55 OC, + 5490.96 K + 1353.56 BD + 752.82 MWD (1) where Y: saffron yield (kgha-1), pH: soil acidity, EC: electoral conductivity (dSm-1), Fe: iron concentration (mgkg-1), Zn: zinc concentration (mgkg-1), OC: organic carbon (%), K: soil potassium (%), BD: soil bulk density (Mgm-3), and MWD: mean weight diameter of aggregates (MM). Based on the absolute values of standard ß in the regression model (Table 3), pH value and then after Zn concentration had the most effect on saffron yield. In general, responses of different plants to soil pH is varied, and saffron grows satisfactory in pH = 7.8 (5). Soil pH influences the uptake of soil nutrients by plants (15), so that this parameter had the most effect on saffron yield and by increasing the soil pH, the yield of saffron decreases. According to the regression model, Zn concentration was the second parameter in saffron yield. Zn has the important role in structure of plant enzymes (30). After these 2 parameters, Bd, MWD, Fe concentration, EC, Organic carbon and K concentration in soil had more effect on saffron yield (Table 3). Conclusion: According to both PCA and regression methods, the concentration of iron and zinc and MWD were determined as the important and effective soil properties on saffron yield in the Ghayenat area. In addition, soil pH in stepwise regression method and calcium carbonate in PCA method were determined as the effective properties on saffron yield. Therefore, it is suggested that the parameters of Zn, Fe, and MWD along with soil pH and calcium carbonate which were regarded individually in two methods, were considered as the most soil properties in saffron yield

Synthesis and structural study of bis-perfluoropyridyl bridged by 1,4 and 1,2 dihydropyridine.

Bis-perfluoropyridyl bridged by 1,4 and 1,2 dihydropyridine compounds was synthesized by reaction of 2 and 4 aminopridine derivatives with pentafluoropyridine. The structures were determined by X-ray crystallography. Compound 3a comprises two crystallographically independent molecules in the asymmetric unit in which one of them shows 1-D infinite chains along [0 1 0] direction due to the intermolecular C–H⋯N hydrogen bonds. In compound 5 intermolecular C–H⋯F and C–H⋯N hydrogen bonds link neighbouring molecules to each other. In addition, in both structures a series of C–F⋯π interactions stabilize the crystal packing

Fluorination and chlorination of nitroalkyl groups

Heterocycles substituted with a nitromethyl (CH2NO2) or phenyl-nitromethyl (CHPhNO2) group were prepared by reaction of a methyl- or phenylmethyl-substituted heterocycle, respectively, with lithium di-isopropylamide followed by quenching the intermediate carbanion with methyl nitrate. Conversion of CH2NO2 attached to an alkyl or aryl moiety into a dichloronitromethyl (CCl2NO2) group was achieved using N-chlorosuccinimide and 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) in dichloromethane. Similarly, CH2NO2 attached to an alkyl or aryl group was converted into difluoronitromethyl (CF2NO2) using either 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate) (Selectfluor™) or N-fluorobenzenesulfonimide with DBU as base and dichloromethane as solvent. Reaction of ω-nitroacetophenone with Selectfluor/DBU in dimethylformamide followed by acidification and distillation gave the parent difluoronitromethane in a useful \u27one-pot\u27 procedure. \ua9 2007 Elsevier Ltd. All rights reserved