Repeat motifs of genomic SSRs and their numbers.

<p>Repeat motifs of genomic SSRs and their numbers.</p

GBS tag coverage for P1 and P2 with overlap across P2 reference genome.

<p>P1 (red) and P2 (blue) GBS tag coverage overlap across the P2 reference sequence.</p

Nucleotide substitute distribution type and counts.

<p>The SNP nucleotide substitution type and count as determined between both genotypes.</p

Comparison between EST- and genomic derived SSRs.

<p>Comparison between EST- and genomic derived SSRs.</p

Genomic SSR discovery pipeline and primer design.

<p>The workflow starts with an independent assembly of each genotype and enrichment for sequence contigs with a length greater than the N50 value. Next, SSRFinder is used to find SSRs and design primers. Possible polymorphisms are detected and screened against the nr database (GenBank) and a priority given to non-repetitive or organellar sequences.</p

Repeat motifs of EST-SSRs and their numbers.

<p>Repeat motifs of EST-SSRs and their numbers.</p

Nucleotide substitute distribution type and counts.

<p>The SNP nucleotide substitution type and count as determined between both genotypes.</p

Image_1_Antagonistic and plant growth promotion of rhizobacteria against Phytophthora colocasiae in taro.tiff

Taro leaf blight caused by Phytophthora colocasiae adversely affects the growth and yield of taro. The management of this disease depends heavily on synthetic fungicides. These compounds, however, pose potential hazards to human health and the environment. The present study aimed to investigate an alternative approach for plant growth promotion and disease control by evaluating seven different bacterial strains (viz., Serratia plymuthica, S412; S. plymuthica, S414; S. plymuthica, AS13; S. proteamaculans, S4; S. rubidaea, EV23; S. rubidaea, AV10; Pseudomonas fluorescens, SLU-99) and their different combinations as consortia against P. colocasiae. Antagonistic tests were performed in in vitro plate assays and the effective strains were selected for detached leaf assays and greenhouse trials. Plant growth-promoting and disease prevention traits of selected bacterial strains were also investigated in vitro. Our results indicated that some of these strains used singly (AV10, AS13, S4, and S414) and in combinations (S4+S414, AS13+AV10) reduced the growth of P. colocasiae (30−50%) in vitro and showed disease reduction ability when used singly or in combinations as consortia in greenhouse trials (88.75−99.37%). The disease-suppressing ability of these strains may be related to the production of enzymes such as chitinase, protease, cellulase, and amylase. Furthermore, all strains tested possessed plant growth-promoting traits such as indole-3-acetic acid production, siderophore formation, and phosphate solubilization. Overall, the present study revealed that bacterial strains significantly suppressed P. colocasiae disease development using in vitro, detached leaf, and greenhouse assays. Therefore, these bacterial strains can be used as an alternative strategy to minimize the use of synthetic fungicides and fertilizers to control taro blight and improve sustainable taro production.</p

Structure of the genetic diversity of the 284 diploid accessions of <i>D</i>. <i>alata</i> at K = 6.

<p>Structure of the genetic diversity of the 284 diploid accessions of <i>D</i>. <i>alata</i> at K = 6.</p

Diagram showing the relationships among 367 accessions of <i>D</i>. <i>alata</i> based on Principal Coordinate Analysis (PCoA) using 24 microsatellites.

<p>Clones originated from Vanuatu are colored in green and those from CTCRI (India) in red. The accessions of the CRB-PT and the IITA are colored in orange and pink, respectively. <b>1B. Diagram showing the relationships among 83 polyploid accessions of <i>D</i>. <i>alata</i> based on Principal Coordinate Analysis(PCoA) using 24 microsatellites.</b> Tetraploids accessions are colored in green and triploids in blue.</p