Glutamine Phosphoribosylpyrophosphate Amidotransferase-independent Phosphoribosyl Amine Synthesis from Ribose 5-Phosphate and Glutamine or Asparagine

Phosphoribosylamine (PRA) is the first intermediate in the common pathway to purines and thiamine and is generated in bacteria by glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase (EC 2.4.2.14) from PRPP and glutamine. Genetic data have indicated that multiple, non-PRPP amidotransferase mechanisms exist to generate PRA sufficient for thiamine but not purine synthesis. Here we describe the purification and identification of an activity (present in both Escherichia coli and Salmonella enterica) that synthesizes PRA from ribose 5-phosphate and glutamine/asparagine. A purification resulting in greater than a 625-fold increase in specific activity identified 8 candidate proteins. Of the candidates, overexpression of AphA (EC 3.1.3.2), a periplasmic class B nonspecific acid phosphatase, significantly increased activity in partially purified extracts. Native purification of AphA to >95% homogeneity determined that the periplasmic L-asparaginase II, AnsB (EC 3.5.1.1), co-purified with AphA and was also necessary for PRA formation. The potential physiological relevance of AphA and AnsB in contributing to thiamine biosynthesis in vivo is discussed

Latino Protestants and Their Political and Social Engagement (Chapter Six of Latino Protestants in America: Growing and Diverse)

Excerpt: On a rainy early spring morning in a modest brick Presbyterian church just outside the Fruitvale neighborhood of Oakland, California, sixtyfour worshippers gather. The entire worship is in Spanish. During the sermon, the pastor makes a passing reference to how few of the attenders now live in Oakland proper, that many have to drive farther than ever for church services. The implicit message: the leadership of the church realizes that gentrification of San Francisco has spilled over the Bay Bridge and now threatens the availability of affordable housing throughout Oakland. In response, the congregation has started programs that offer legal advice for responding to rent-hiking landlords and identifying housing options around the city. Though resources and attenders tend to be somewhat scarce, the leadership has creatively organized in an effort to address the structural and policy concerns of housing. Beyond that, the pastor proudly notes that this church readily offers immigration status services, computer classes, and English classes

Why Are some Filipino Children not in School?

What are the factors that contribute to the nonparticipation of children in school? Who are the ones accountable to this complicating issue? This Note identifies some factors and looks deeper into each of them.poverty, education, Philippines, carbon tax, carbon dioxide emission, school participation, out-of-school children

Some Issues on Low Participation Rates in Basic Education

Despite the various efforts to improve the Philippines` education system, why does this country still have low participation rates in education? What are the reasons behind the growing numbers of out-of-school children? What are the reasons for their nonattendance? This Note delves deeper into the issue.Philippines, basic education, school participation, out-of-school children

Valuation and modeling of EQ-5D-5L health states using a hybrid approach

Background: The EQ-5D instrument is the most widely used preference-based health-related quality of life questionnaire in cost-effectiveness analysis of health care technologies. Recently, a version called EQ-5D-5L with 5 levels on each dimension was developed. This manuscript explores the performance of a hybrid approach for the modeling of EQ-5D-5L valuation data. Methods: Two elicitation techniques, the composite time trade-off, and discrete choice experiments, were applied to a sample of the Spanish population (n=1000) using a computer-based questionnaire. The sampling process consisted of 2 stages: stratified sampling of geographic area, followed by systematic sampling in each area. A hybrid regression model combining composite time trade-off and discrete choice data was used to estimate the potential value sets using main effects as starting point. The comparison between the models was performed using the criteria of logical consistency, goodness of fit, and parsimony. Results: Twenty-seven participants from the 1000 were removed following the exclusion criteria. The best-fitted model included 2 significant interaction terms but resulted in marginal improvements in model fit compared to the main effects model. We therefore selected the model results with main effects as a potential value set for this methodological study, based on the parsimony criteria. The results showed that the main effects hybrid model was consistent, with a range of utility values between 1 and -0.224. Conclusion: This paper shows the feasibility of using a hybrid approach to estimate a value set for EQ-5D-5L valuation data.</p

Ariel - Volume 12 Number 2

Executive Editors David G. Polin Larry H. Pastor Business Manager Alex Macones Jean Lien Editorial Page Editor Deepak Kapoor Sports Editor Todd Hoover Photography Editors Lois Leach Ken Yonemur

Mobilizing Undergraduates to Address the Social Determinants of Health in the Community: Year 1 of Health Leads at UMass Boston

Health Leads, a national non-profit organization, currently operates in six cities. Following successful partnerships with Harvard University and Boston University, Health Leads Boston initiated a partnership with UMass Boston in the Fall of 2012 to mobilize undergraduate students in addressing the social determinants of health for patients at Codman Square Health Center (CSHC). 16 undergraduates at UMass Boston were selected into the Health Leads program at CSHC, joining 17 students from Harvard and 4 students from BU. In 6 months, Health Leads at CSHC served 337 families, with students reporting positive experiences. Further expansion of Health Leads at UMB is planned with efforts focused on integrating the program on campus

Do neighbourhood environmental perceptions affect practices?

In this paper, we examine how environmental practices related to public transit and urban green space use are influenced by perceptions of local level environmental change, neighbourhood inhabitation, and socio-demographic factors. The analysis shows that perceptions of change and neighbourhood inhabitation offer better explanations for changing local environmental practices than socio-demographic orientations. We contribute to social practice theory by drawing attention to the interplay of environmental perceptions and neighbourhood inhabitation as factors that facilitate changing environmental practices. By gaining insight into the relationship between perceptions of change and environmental practices, we thereby learn how sustainability goals, such as those embodied by SDG11, can be translated into social practices at the community level.Dans cet article, nous examinons comment les pratiques environnementales liées au transport en commun et à l’utilisation des espaces verts urbains sont influencées par les perceptions du changement environnemental au niveau local, l’habitation des quartiers et les facteurs sociodémographiques. L’analyse montre que les perceptions du changement et de l’habitat du quartier offrent de meilleures explications pour l’évolution des pratiques environnementales locales que les orientations sociodémographiques. Nous contribuons è la théorie de la pratique sociale en attirant l’attention sur l’interaction des perceptions environnementales et de l’habitation du quartier en tant que facteurs qui facilitent l’évolution des pratiques environnementales. En acquérant un aperçu de la relation entre les perceptions du changement et les pratiques environnementales, nous apprenons ainsi comment les objectifs de durabilité, tels que ceux incarnées par ODD (Agenda 2030 du développement durable), peuvent être traduits en pratiques sociales au niveau communautaire