ANTIBIOGRAM AND MOLECULAR CHARACTERIZATION OF VIBRIO CHOLERAE ISOLATED FROM MARINE FISH

Objective: Molecular identification and antibiotic susceptibility evaluation of Vibrio cholerae from marine fish available in local fish market Thanjavur, Tamil nadu, India.Methods: inoculation was done by using nutrient agar as general media and TCBS agar as selective media and confirmed as V.cholerae by Gram stain (Microscopic Observation), Growth characteristics of different media, Biochemical tests like Methyl Red Test, Nitrate Reduction Tests, Indole Test etc. Sensitivity (drug sensitivity) was done in Muller Hinton Agar (MH Agar) using disc diffusion method ten different antibiotics were used to evaluate the antibiogram profile, molecular detection was done by targeting 16S rRNA gene by using a universal primer.Results:  V.cholerae is present in marine fish samples, as showed by culture method and microscopic observation as well biochemical tests. PCR amplification of 16S rRNA gene showed the amplification of targeted gene and antibiogram profile showed that isolates are more sensitive to Ampicillin in comparison with others antibiotics used in this study. Ampicillin can be used for V.cholerae infection by the physicians and amoxicillin must be avoided which is resistant.Conclusion: Molecular detection is safe and rapid methods for bacteria identification as revealed by PCR amplification of 16S rRNA gene. As the isolates are more sensitive to Ampicillin in comparison with others antibiotics used in this study. Ampicillin can be used for V.cholerae infection by the physicians and amoxicillin and nitrofurantoin must be avoided.     Â

Complexes of Arginine, Histidine, Lysine & Isoleucine with Potassium Hexacyanoferrate(III)

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Complexes of Arginine, Histidine, Lysine & Isoleucine with Potassium Hexacyanoferrate(III)

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Epiphenomenon of telomere lengths : lessons from ulcerative colitis

We read with great interest the article by Jones et al detailing TERC polymorphisms, longer telomeres and increased risk of colorectal cancer (CRC).1 Indeed, it is accepted that shorter telomere lengths (measured from peripheral leucocytes) have been shown in senescent somatic cells and also to predispose to cancer. The biological rationale is that telomeres which form a protective cap at the end of chromosomes can be disrupted to undergo double strand breaks, inefficient repair and eventually chromosomal instability. The latter is a well-established precursor to cancer development. Thus, the authors' finding of longer telomeres associated with risk of CRC is particularly intriguing

Selective measurement of anti-tTG antibodies in coeliac disease and IgA deficiency : an alternative pathway

Objective To determine the ability of selective antibody testing to screen for coeliac disease in the presence of IgA deficiency and to define the sensitivity of a pathway using this method (Figure1). Method All IgA and IgG anti-tTG tests performed at our centre between January 2008 and December 2009, using the Immunocap 250 analyser, were retrospectively reviewed. Positive results were correlated with histology. Results were used to validate our diagnostic pathway. Results 12,289 consecutive serological tests were reviewed. IgA deficient patients gave either an “error” reading or very low response on the Immunocap 250 analyser. Subsequent testing of this sub-group demonstrated raised IgG anti-tTG antibodies in those with histologically proven coeliac disease. Conclusions Using our antibody screening pathway, which involves the selective use of IgG antitTG, sensitivity increased from 87% to 92% in those with IgA deficiency. Adoption of this pathway for coeliac screening would negate the routine screening of immunoglobulin levels, with resultant cost saving

Infrared & Mossbauer Spectral Studies on Organic Base Hydroferrocyanides

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Polaroaraphic & Spectral Studies on the Reaction of Fe(III) with Pentacyanonitrosylmanganate

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Certain seasonal characteristic features of oceanic heat budget components over the indian seas in relation to the summer monsoon activity over India

The present study has been undertaken to examine the oceanic heat budget components and their variability over the Indian seas in relation to the extreme monsoon activity (flood/drought) over the Indian subcontinent. For this purpose, various components of oceanic heat budget have been analysed for pre-monsoon (March-May), monsoon (June-September), post-monsoon (October-December) and winter (January-February) seasons over India. The data base used in this study consists of mean monthly marine meteorological fields for 30 years (1950-1979), which is a part of the Comprehensive Ocean Atmosphere Data Set (COADS) analysed at 1° latitude by 1° longitude resolution. The mean fields of incoming shortwave radiation flux over the Indian seas in different seasons vary in accordance to the sun's position and cloud cover variations. The latent heat flux undergoes considerable seasonal variations, particularly over the Arabian Sea. There is a dominance of latent heat flux (representing the oceanic heat loss) over shortwave solar flux (representing the oceanic heat gain) during the monsoon season, which results in a zone of net oceanic heat loss over the central Arabian Sea. This feature produces a positive feedback for the maintenance of deep cumulus convection over the Arabian Sea in this season. It is also found that oceanic heat budget components over the Indian seas exhibit significant variability in relation to the extreme monsoon activity leading to flood/drought over India. Based on the above analysis, mean monthly variations of the oceanic heat budget components over three smaller sectors of the Indian seas, namely west equatorial Indian Ocean, Arabian Sea and Bay of Bengal, are also examined. The variations are found to be considerably different between the two extreme categories of the monsoon. It becomes evident from this study that the oceanic sectors of west equatorial Indian Ocean and Bay of Bengal experience a large reduction of net heat flux from April to May prior to a flood monsoon season over India. This suggests that a spectacular increase of latent heat flux and decrease of shortwave flux occurs over these oceanic sectors, leading to a sharp reduction of net heat flux