Creating Difference: The Legal Production of Race in American Slavery

This dissertation examines the legal construction and development of racial difference as considered in literature written or set during the final years of American slavery. While there had consistently been a conceptual correspondence between black skin and enslavement, race or racial difference did not become the unqualified explanation of enslavement until fairly late in the institution’s history. Specifically, as slavery’s stability became increasingly threatened through the nineteenth century by abolitionism and racial slippage, race became the singular and explicit rationale for its existence and perpetuation. I argue that the primary discourse of this justificatory rationale was legal: through law race and its meaning was finally determined. However, as there was not a substantial body of legal texts, such as legislation and judicial opinions, defining race prior, legal determinations of race during this brief and tumultuous period ultimately produced race in service of slavery. To frame and understand these legal issues, my dissertation turns to nineteenth-century American literature. Because of the elusive nature of racial difference, literature provides a means to reflect upon and critique the law’s complicity in producing race. I begin with an overview of the difficulty of understanding and defining race as a concept, as well as the reasons why and when race became definitive of American slavery. I then turn to Frederick Douglass’s My Bondage and My Freedom (1855) which explores how race came to signify wrongdoing as a consequence of race-based slavery. I then discuss how knowledge of racial difference, characterized by its uncertainty, was stabilized by gender. As considered in Lydia Maria Child’s 1867 novel, A Romance of the Republic an individual’s uncertain racial identity was legally determined by recourse to his or her mother’s. The next chapter examines Mark Twain’s Pudd’nhead Wilson, published in 1894, which refutes the very existence of a racial difference, suggesting its legal existence is arbitrarily produced for the purposes of slavery. Finally, I conclude with a consideration of the limits of, and unexplored issues raised by, this project

Primitive dreams: In search of judgment

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SNP and Haplotype characterisation of Apobec 3G, a protein invovled in Retroviral defence, in Black South Africans

Heritable variation is important in disease progression, therefore its association with HIV/AIDS was analyzed. APOBEC3G is a unique cellular gene that influences HIV infectivity. It belongs to family of cytidine deaminases and is both an RNA and DNA editing enzyme. APOBEC3G is a good candidate for HIV restriction because it allows the expression of an antiviral phenotype in non-permissive cells consequently this innate immune defense may provide the basis for the design of new therapies for HIV. Variation in the upstream non-coding region of APOBEC3G was studied. Six base variants were found at positions -90, -163, -166, -571, -590 and -821. In addition, promoter analysis identified promoters in the upstream non-coding region. Indirect genotyping assays were developed to genotype the participants at -571 and H186R. The frequency of -571 GG was 70 %. The frequency of the TT genotype of H186R was 20 %. The GG genotype was selected against in the HIV + group of the study participants. This is indicative that this SNP has disease modifying effects. The TT genotype was related to increased progression to AIDS confirming the results of previous studies

SNP and haplotype characterisation of apobec 3G, a protein involved in retroviral defence, in Black South Africans

A dissertation submitted to the Faculty of Science, University of the Witwatersrand, in fulfillment of the requirements for the degree of Master of Science Johannesburg, August 2012It is known that infectious agents elicit different responses in different individuals which strengthens the view that susceptibility and resistance to infectious diseases has a genetic component. These differences in susceptibility to disease can be observed in populations. APOBEC3G is a member of the cytidine deaminase gene family located on chromosome 22. It is crucial in non-permissive cells as it functions as part of the innate immunity system and is an inhibitor of the HIV-1 accessory protein vif. The goal of the study was to develop genotyping assays and estimate allele frequencies. Thus, genetic variation within APOBEC3G was identified and characterized in black South Africans. Indirect genotyping assays were designed to amplify regions within the upstream non-coding region, and in exon 4 of the coding region of the gene. Selected polymorphisms were then genotyped using allele-specific PCR, RFLP-PCR and Pyrosequencing™ assays. Reanalysis of sequence data from 2003 showed numerous SNPs were well represented. Comparison of sequence data at various SNPs showed that allele frequencies were similar to frequencies in other African populations. The only sequenced SNP that deviated from the frequencies in Ensembl was -590. Thus the sequencing was a useful tool for detection of variation. ASA proved to be the least reliable genotyping technique as the minor allele frequency of -571 (0.59) deviated from the published frequency of 0.894 in Africans. RFLP analysis proved more reliable for genotyping -571 and H186R. The minor allele frequency was estimated to be 0.84 and 0.32 for -571 and H186R respectively. The frequency of H186R is similar to published data from An et al (2004) and Reddy et al (2010). If SNPs are in LD they occur together on the same haplotype more often than by chance. Usually SNPs that are in LD are in close proximity. However our data suggests -571 and H186R SNPs which are 5kb apart are not in LD. A LD map of chromosome 22 shows highly variable pattern of LD (Dawson et al, 2002). Widespread regions of nearly complete LD up to 804 kb in length are intermingled with regions of little or uundetectable LD. Haplotype analysis showed the most frequent haplotype was GA. This was the most frequent haplotype when the sample types were subdivided according to spoken language. in comparison to studies from An et al, (2004) D’ of the two SNPs was estimated at 0.967. The linkage disequilibrium (LD) revealed a non-independence of allele segregation because the loci analyzed were strongly linked in the Apobec 3 G gene. The data are consistent with greater genetic diversity of African populations and can form the basis for further evaluation of the role of variation in this gene in response to HIV

The relationship between identity processing style and academic performance of first year psychology students.

Academic performance of first year university students in the international arena as well as locally, has been a point of concern for all stakeholders because of high dropout rates and failure. Although many explanations for this have been offered and accepted, all have located the problem external to the individual. This study examined the interplay between interpersonal and intrapersonal factors on academic performance of first year university students in South Africa. A sociocognitive perspective was employed by an investigation of student identity processing styles as a means to explain academic performance. A mixed sample of 419 first year psychology students at a South African university was randomly chosen. Berzonsky’s Identity Style Inventory (ISI3) was used to categorise students’ identity processing styles which was then correlated to students’ mid-year examination results. Although similar research was conducted overseas, the findings of the present study did not match previous results. Unlike any other known study the correlation between normative processing style and academic performance of first year university students was statistically significant but was negative. There was significant difference only between informational and normative identity processing styles on academic performance and between informational and diffuse-avoidant processing styles on academic performance. It was found in this study that culture and race played a role in student identity processing styles and in turn influenced student academic performance in the first year of university. A discussion of results, educational implications of findings, limitations of the study and recommendations for future research are included at the end of this study

A review of antenatal corticosteroid use in premature neonates in a middle-income country

Background. Antenatal corticosteroid (ANS) use in premature neonates has become a standard of practice. However, there is low ANS coverage in low- to middle-income countries (LMICs). Recent studies have questioned the efficacy of ANSs in such countries.Objective. To review the use of ANSs in preterm neonates at Charlotte Maxeke Johannesburg Academic Hospital (CMJAH), South Africa.Methods. This was a retrospective observational study of all neonates with a birth weight of 500 - 1 800 g born at CMJAH between 1 January 2013 and 30 June 2016. Neonatal and maternal characteristics of neonates exposed to ANSs were compared with those of neonates who were not exposed.Results. The ANS coverage of the final sample was 930/2 109 (44.1%). The mean (standard deviation (SD)) birth weight was 1 292.4 (323.2) g and the mean gestational age 30.2 (2.9) weeks. Attending antenatal care and maternal hypertension were associated with increased use of ANSs, whereas vaginal delivery was associated with decreased use. In neonates weighing <1 500 g, the use of ANSs was associated with decreased mortality, decreased intraventricular haemorrhage and decreased patent ductus arteriosus. There was no association between ANSs and respiratory distress syndrome, necrotising enterocolitis, sepsis or need for respiratory support in all premature neonates, and no association with improved outcomes in those weighing ≥1 500 g.Conclusion. The benefits of ANSs in terms of neonatal morbidity in this study were not as marked as those published in high-income countries. A randomised controlled trial may be indicated in LMICs

Gaucher disease: A cause of massive splenomegaly in a 15-year-old black African male

Patients with Gaucher disease (GD), a rare autosomal recessive lysosomal storage disease, commonly present to paediatricians with massive splenomegaly. While the diagnosis and management of patients with this chronic multisystem disorder has evolved significantly in recent years, the initial diagnosis represents a challenge. We describe the case of a 15-year-old black African male who presented with abdominal distension, delayed growth and fatigue. Initial laboratory studies revealed severe anaemia (haemoglobin concentration 8 g/dL) and moderate thrombocytopenia (platelet count 80 × 109/L). A computed tomography scan of the abdomen showed an enlarged liver of 173 mm and massive splenomegaly of 27 mm. The diagnosis of GD was confirmed by reduced beta-glucocerebrosidase activity and heterozygous mutations in the GBA1 gene. The patient was managed at a dedicated paediatric haematology unit with enzyme replacement therapy and regular clinical, biochemical and radiological monitoring