Differing Effects of Glycerin on Anaerobic Co-digestion of Mixed Substrates in Bench-Scale Assays and Sub Pilot-Scale Reactors

Bench-scale methods such as Biochemical Methane Potential (BMP) assays and Anaerobic Toxicity Assays (ATAs) are useful tools in evaluating potential feedstocks for anaerobic digestion. The BMP method provides a preliminary indication of substrate biodegradability and methane production, while the ATAs provide an indication of substrate toxicity to anaerobic microbial consortia. Previous research using small (\u3c20 \u3eL) reactors indicated that co-digestion of manures with small amounts of glycerin (ca. 1 – 2 %) can double methane production, but toxicity can result if glycerin exceeds 2% (volumetric basis). This paper investigated the relationship between bench-scale methods (BMPs and ATAs) and sub pilot-scale digester results, using glycerin as a test substrate mixed with a baseline feedstock (beef manure, corn processing wastewater, lagoon liquid, and short-fiber cardboard). The batch-fed, stirred ATAs indicated that glycerin was toxic to methane production at all inclusion levels. The batch-fed, stirred BMPs indicated no significant difference between methane production in the 0.0%to 4.0% addition levels; however at 8.0% addition, methane production tripled. The continuously fed, non-stirred, plug-flow sub pilot-scale reactors indicated toxicity effects in the 2.0% and 4.0% glycerin mixtures and no difference from the control in the 1.0% glycerin mixture. These results demonstrate the variations in scale performance of glycerin as a co-substrate and identify some serious challenges in extrapolating bench-scale assays to large-scale performance of mixed-waste anaerobic digestion systems

Approaches for Selecting Anaerobic Digestion Co-Substrates for a Full-Scale Beef Manure Digester Using Biochemical Methane Potentials and Anaerobic Texicity Assays

Design and construction of full-scale anaerobic digesters that co-digest manure with various materials requires analysis of each substrate. Substrate combinations should be analyzed through a scale up procedure in which substrates are characterized, and then evaluated using biochemical methane potential assays (BMPs) and anaerobic toxicity assays (ATAs). The BMPs provide a preliminary indication of the biodegradability of a substrate and of its potential to produce methane via anaerobic digestion, while ATAs determine the degree to which a particular substrate inhibits methane production. Mixture combinations that perform well in BMPs and ATAs should be tested in laboratory-scale anaerobic digesters. Once proven in lab-scale reactors for at least three hydraulic retention times, the best mixture should be tested in a pilot-scale reactor. This paper focuses on the first steps in this process using BMPs and ATAs results to select mixtures for laboratory-scale digester testing. The baseline feedstock was beef manure obtained from concrete feedlot pens (open and covered) in eastern Iowa. Various bedding materials were available, including oat hulls, corn stover, and wood shavings. To provide additional energy production, industrial byproducts from cardboard manufacturing, enzyme production, and corn and soybean processing were also potential substrates. Substrates were characterized for TS, VS, COD, pH, alkalinity, and ammonia. Then BMPs were completed on all substrates and ATAs were performed as needed. The results reported here were used to develop mixtures for use in laboratory-scale anaerobic digester testing

Comparison of Methane Production from Bench- and Sub Pilot-Scale Anaerobic Digesters

Design and construction of full-scale anaerobic digesters that co-digest manure with other substrates, such as food processing wastes, is challenging because of the large number of potential mixtures that can be fed to the digester. In this work we examine the relationship between results from bench-scale methods such as biochemical methane potential assays (BMPs) and sub pilot-scale reactors. The baseline feedstock for this study was beef manure from concrete feedlot pens (open and covered) in eastern Iowa. Additional co-digestion substrates tested were short-fiber cardboard, corn processing wastewater, enzyme processing wastewater and lagoon liquid. Substrates were characterized for total solids (TS), volatile solids (VS), chemical oxygen demand (COD), pH, alkalinity, and ammonia, after which BMPs were conducted on all substrates. Based on the BMP and anaerobic toxicity assay (ATA) results, a mixture was created and evaluated using BMPs and tested in 100-L sub pilot-scale reactors. This study showed that results from BMPs of feedstock co-digestion mixtures accurately estimated the range of methane produced from three 100-L, plug flow reactors