Endophyte Microbiome Diversity in Micropropagated Atriplex canescens and Atriplex torreyi var griffithsii

Microbial diversity associated with micropropagated Atriplex species was assessed using microscopy, isolate culturing, and sequencing. Light, electron, and confocal microscopy revealed microbial cells in aseptically regenerated leaves and roots. Clone libraries and tag-encoded FLX amplicon pyrosequencing (TEFAP) analysis amplified sequences from callus homologous to diverse fungal and bacterial taxa. Culturing isolated some seed borne endophyte taxa which could be readily propagated apart from the host. Microbial cells were observed within biofilm-like residues associated with plant cell surfaces and intercellular spaces. Various universal primers amplified both plant and microbial sequences, with different primers revealing different patterns of fungal diversity. Bacterial and fungal TEFAP followed by alignment with sequences from curated databases revealed 7 bacterial and 17 ascomycete taxa in A. canescens, and 5 bacterial taxa in A. torreyi. Additional diversity was observed among isolates and clone libraries. Micropropagated Atriplex retains a complex, intimately associated microbiome which includes diverse strains well poised to interact in manners that influence host physiology. Microbiome analysis was facilitated by high throughput sequencing methods, but primer biases continue to limit recovery of diverse sequences from even moderately complex communities

One foot in the rave: Ageing ravers’ transitions to adulthood and their participation in rave culture

Challenging adolescence limited life-course theory, this article presents the results of an online survey (2016) that aimed to establish if, how and to what extent ageing ravers continue to participate in rave culture. The survey collected data from members of the original rave generation. We explored the ravers’ transitions to adulthood, and how these influenced their participation in club culture. We found that our ravers continued to participate in rave culture; their persistence is related to the frequency of their engagement at a younger age. Adopting interdisciplinarity, we apply theories from both life course research and youth culture studies and argue that the persistence of leisure practices, such as raving, can be understood in the same way as persistence of drug use: frequent behavior in adolescence determines persistence in older adulthood. Consequently, we are able to recognize patterns of leisure beyond adolescence, thus making leisure choices more predictable

Notes for genera: basal clades of Fungi (including Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota)

Compared to the higher fungi (Dikarya), taxonomic and evolutionary studies on the basal clades of fungi are fewer in number. Thus, the generic boundaries and higher ranks in the basal clades of fungi are poorly known. Recent DNA based taxonomic studies have provided reliable and accurate information. It is therefore necessary to compile all available information since basal clades genera lack updated checklists or outlines. Recently, Tedersoo et al. (MycoKeys 13:1--20, 2016) accepted Aphelidiomycota and Rozellomycota in Fungal clade. Thus, we regard both these phyla as members in Kingdom Fungi. We accept 16 phyla in basal clades viz. Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Calcarisporiellomycota, Caulochytriomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, Kickxellomycota, Monoblepharomycota, Mortierellomycota, Mucoromycota, Neocallimastigomycota, Olpidiomycota, Rozellomycota and Zoopagomycota. Thus, 611 genera in 153 families, 43 orders and 18 classes are provided with details of classification, synonyms, life modes, distribution, recent literature and genomic data. Moreover, Catenariaceae Couch is proposed to be conserved, Cladochytriales Mozl.-Standr. is emended and the family Nephridiophagaceae is introduced

Continued food aversion: Training livestock to avoid eating poisonous plants

Paper presented at the "Symposium on Ingestion of Poisonous Plants by Livestock," February 15, 1990, Reno, Nevada.Animals can be trained to avoid eating specific foods by offering them the food and subsequently administering an emetic to induce nausea. The animal associates the taste of the food with the induced illness and subsequently avoids eating that food. Conditioned food aversion (CFA) is a potential tool to prevent livestock poisoning from palatable and abundant poisonous plants. Cattle have been trained to avoid eating tall larkspur (Delphinium barbeyi L. Huth), a particularly troublesome poisonous plant. However, several factors influence the acquisition and retention of food aversions under field grazing conditions. The age and sex of an animal may influence its ability to form and retain aversions. Novelty of the plant and the intensity of the induced illness determine the strength of the aversion. Social facilitation or peer pressure motivates animals to sample the averted food, and the aversion will extinguish if it is not reinforced. Generalizing the aversion created under controlled conditions in a pen, to a complex vegetation community in the field, may be difftcult for some animals. If these obstacles can be overcome, CFA may be an effective tool to reduce the risk of poisoning on poisonous plant infested rangeland.This material was digitized as part of a cooperative project between the Society for Range Management and the University of Arizona Libraries.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform August 202

Persistence of aversions to larkspur in naive and native cattle

The objective of this study was to create and maintain a long lasting aversion to tall larkspur (Delphinium barbeyi Huth) under field grazing conditions. Two grazing trials were conducted: the first used cattle naive to larkspur, and the second used cattle that were familiar with larkspur. In the first trial, 12 mature cows that were not familiar with larkspur were randomly allocated to Averted and Control groups (n = 6). They were fed larkspur (a novel food) and the Averted group was dosed with lithium chloride (200 mg/kg body weight) to create the aversion. Both groups were then taken to larkspur-infested mountain rangeland where they grazed in 2 separate pastures during the late summer of 1993, 1993, 1994, and 1995. The aversion was not reinforced in 1994 or 1995. Diets were quantified by bite count. The Averted group abstained from eating larkspur for 3 years. The Control group grazed larkspur for an average of 14% of bites. Three Control cows died from larkspur poisoning in 1993, and another cow was poisoned, but survived in 1994. During the last 2 weeks of the 1995 trial, the Averted and Control groups were placed together, and the social influence of the Control cows eating larkspur caused the Averted cows to sample larkspur and gradually extinguished the aversion. In the second trial (1994 and 1995), 5 native cows that had grazed on the allotment and were familiar with larkspur were averted to larkspur by the procedure described above. They abstained from eating larkspur while grazing separately, but extinguished the aversion when placed with non-averted Control cows at the end of the study in 1995. Aversions are retained in long term memory and may last indefinitely if averted cattle graze separately.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform August 202

Ecological relationships between poisonous plants and rangeland condition: A Review

In the past, excessive numbers of livestock on western U.S. rangelands, reoccurring droughts, and lack of management resulted in retrogression of plant communities. Poisonous plants and other less palatable species increased with declining range condition and livestock were forced to eat these poisonous species because of a shortage of desirable forage, resulting in large, catastrophic losses. The level of management on most western rangelands has improved during the last 60 years, resulting in marked improvement in range condition; yet losses to poisonous plants still occur, though not as large and catastrophic as in the past. Some poisonous species are major components of the pristine, pre-European plant communities [tall larkspur (Delphinium barbeyi Huth), Veratrum californicum Durand, water hemlock (Cicuta douglasii (DC.)Coult. Rose), bracken fern (Pteridium aquilinum (L.) Kuhn), chokecherry (Prunus virginiana L.), Ponderosa pine (Pinus ponderosa Lawson), and various oak species (Quercus spp.)]. Although populations of many poisonous seral increaser species have declined with better management, they are still components of plant communities and fluctuate with changing precipitation patterns [locoweed (Astragalus and Oxytropis spp.), lupine (Lupinus spp.), death camas (Zigadenus spp.), snakeweed (Gutierrezia spp.), threadleaf groundsel (Senecio longolobis Benth.), low larkspur (Delphinium nuttallianum Pritz.), timber milkvetch (Astragalus miser Dougl. ex Hook.), redstem peavine (A. emoryanus (Rydb.) Cory), western bitterweed (Hymenoxys odorata D.C.), orange sneezeweed (Helenium hoopesii Gray), twin leaf senna (Cassia roemeriana Schelle), and white snakeroot (Eupatorium rugosum Houtt)]. Many of the alien invader species are poisonous: [Halogeton glomeratus (Bieb.) C.A. Mey, St. Johnswort (Hypericum perforatum L.), poison hemlock (Conium maculatum L.), tansy ragwort (Senecio jacobaea L.), hounds tongue (Cynoglossum officinale L.), leafy spurge (Euphorbia esula L.), yellow star thistle (Centaurea solstitialis L.) and other knapweeds (Centaurea spp.)]. Poisoning occurs when livestock consume these plants because they are either relatively more palatable than the associated forage, or from management mistakes of running short of desirable forage.The Journal of Range Management archives are made available by the Society for Range Management and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform August 202