Artificial neural network–genetic algorithm-based optimization of biodiesel production from Simarouba glauca

A transesterification reaction was carried out employing an oil of paradise kernel (Simarouba glauca), a non-edible source for producing Simarouba glauca methyl ester (SGME) or biodiesel. In this study, the effects of three variables – reaction temperature, oil-to-alcohol ratio and reaction time – were studied and optimized using response surface methodology (RSM) and an artificial neural network (ANN) on the free fatty acid (FFA) level. Formation of methyl esters due to a reduction in FFA was observed in gas chromatography–mass spectroscopy (GC–MS) analysis. It was inferred that optimum conditions such as an oil-to-alcohol ratio of 1:6.22, temperature of 67.25 and duration of 20 h produce a better yield of biodiesel with FFA of 0.765 ± 0.92%. The fuel properties of paradise oil meet the requirements for biodiesel, by Indian standards. The results indicate that the model is in substantial agreement with current research, and simarouba oil can be considered a potential oil source for biodiesel production

Design and Motion Planning of a Wheeled Type Pipeline Inspection Robot

The most popular method for transporting fluids, and gases is through pipelines. For them to work correctly, regular inspection is necessary. Humans must enter potentially dangerous environments to inspect pipelines. As a result, pipeline robots came into existence. These robots aid in pipeline inspection, protecting numerous people from harm. Despite numerous improvements, pipeline robots still have several limitations. This paper presents the design and motion planning of a wheeled type pipeline inspection robot that can inspect pipelines having an inner diameter between 250 mm to 350 mm. The traditional wheeled robot design has three wheels fixed symmetrically at a 120° angle apart from each other. When maneuvering through a curved pipeline, this robot encounters motion singularity. The proposed robot fixes the wheels at different angles to address this issue, allowing the robot to stay in constant contact with the pipe's surface. Motion analysis is done for the proposed and existing robot design to study their behavior inside the pipeline. The result shows that the proposed robot avoids motion singularity and improves mobility inside pipelines. 3d printing technology aids in the development of the proposed robot. The experimental tests on the developed robot inside a 300 mm-diameter straight and curved pipeline show that the robot avoids motion singularity

Amino­guanidinium hydrogen fumarate

The title compound, CH7N4 +·C4H3O4 −, is a molecular salt in which the amino­guanidinium cations and fumarate monoanions are close to planar, with maximum deviations of 0.011 (1) and 0.177 (1) Å, respectively. The crystal packing is stabilized by inter­molecular N—H⋯O and O—H⋯O hydrogen bonds

Resistome and mobilome in surface runoff from manured soil as affected by setback distance

Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a setback distance of ~40 m is required to reduce the total abundance of ARGs and MGEs in runoff from amended plots to that in control runoff. The resistome and mobilome of the soil in the setback region was not affected by manure-borne ARGs and MGEs. SourceTracker analyses revealed that background soil gradually became the predominant source of the ARGs and MGEs in runoff as setback distance increased. The results demonstrate how manure-borne ARGs and MGEs dissipated in agricultural runoff with increasing setback distance and had limited impacts on the resistome and mobilome of soil within the setback region

Predictors of unfavorable responses to therapy in rifampicin-sensitive pulmonary tuberculosis using an integrated approach of radiological presentation and sputum mycobacterial burden

INTRODUCTION: Despite the exalted status of sputum mycobacterial load for gauging pulmonary tuberculosis treatment and progress, Chest X-rays supplement valuable information for taking instantaneous therapeutic decisions, especially during the COVID-19 pandemic. Even though literature on individual parameters is overwhelming, few studies have explored the interaction between radiographic parameters denoting severity with mycobacterial burden signifying infectivity. By using a sophisticated approach of integrating Chest X-ray parameters with sputum mycobacterial characteristics, evaluated at all the three crucial time points of TB treatment namely pre-treatment, end of intensive phase and completion of treatment, utilizing the interactive Cox Proportional Hazards model, we aimed to precisely deduce predictors of unfavorable response to TB treatment. MATERIALS AND METHOD: We extracted de-identified data from well characterized clinical trial cohorts that recruited rifampicin-sensitive Pulmonary TB patients without any comorbidities, taking their first spell of anti-tuberculosis therapy under supervision and meticulous follow up for 24 months post treatment completion, to accurately predict TB outcomes. Radiographic data independently obtained, interpreted by two experienced pulmonologists was collated with demographic details and, sputum smear and culture grades of participants by an independent statistician and analyzed using the Cox Proportional Hazards model, to not only adjust for confounding factors including treatment effect, but also explore the interaction between radiological and bacteriological parameters for better therapeutic application. RESULTS: Of 667 TB patients with data available, cavitation, extent of involvement, lower zone involvement, smear and culture grade at baseline were significant parameters predisposing to an unfavorable TB treatment outcome in the univariate analysis. Reduction in radiological lesions in Chest X-ray by at least 50% at 2 months and 75% at the end of treatment helped in averting unfavorable responses. Smear and Culture conversion at the end of 2 months was highly significant as a predictor (p2 zones, were 3.05 (95% CI: 1.12–8.23) and 1.92 (95% CI: 0.72–5.08) respectively. Patients without cavitation, zonal involvement 2 zones and 3+ smear grade individually and independently forecasted a poorer TB outcome. The interaction model revealed that Zonal involvement confined to 2 zones, without a cavity and smear grade up to 2+, constituting “minimal disease”, had a better prognosis. Radiological clearance >50% along with smear conversion at the end of intensive phase of treatment, observed to be a reasonable alternative to culture conversion in predicting a successful outcome. These parameters may potentially take up key positions as stratification factors for future trials contemplating on shorter TB regimens

Mandibular Talon Cusp in Primary Lateral Incisor: A Rare Case Report

A talon cusp is a dental anomaly commonly occurring in the permanent dentition compared to the primary dentition. It commonly affects the maxillary anterior teeth. In primary dentition, the most commonly affected tooth is the maxillary central incisors. This is a rare case report of a 5-year-old male patient with a talon cusp affecting the mandibular primary lateral incisor. Recognition and treatment of this anomaly at early stages is important to avoid complications

Molecular Characterisation of Multidrug Resistant Pathogen Isolated from Egg and its Control Measures

Multidrug resistance is a condition enabling a disease causing microorganism to resist distinct drugs or chemical of wide variety of structure and function targeted at eradicating the bacteria. Microorganisms that display multidrug resistant can be pathogenic cells. In this study, table eggs are used commonly, because it considered the most nutritious economical source of protein that can be a part of healthy diet. However, egg shell carries bacteria which cause illness; even unbroken clean fresh shell egg may also contain harmful bacteria. In egg shell presence of Escherichia coli and Staphylococcus aureus were found. Out of total examined sample, no Salmonella was detected. Several disease occurred in poultry are caused by Staphylococcus sp. such as Staphylococcus aureus. It produces Enterotoxins which create food poisoning in consumers. Eggs are the potential source of transmitting antibiotics. Resistant Staphylococcus strain to human causing food-borne infection through Staphylococcus strains can potentially be harmful to humans. Methicillin-resistant Staphylococcus aureus (MRSA) is considered as one of the important bacterium. As a result, Multidrug resistance (MDR) in the bacteria may develop. Nowadays poultry farm increase day by day in Tamilnadu, there is a chance of transmitting the MRSA to Human through egg consumption. This present study was conducted to isolate the bacterial species and the characterization of Staphylococcus aureus along with remedial measure on egg Shell surface. Keywords: Multidrug resistant, Pathogenic, Egg shell, Antibiotics, Human welfar

Five Canalled and Three-Rooted Primary Second Mandibular Molar

A thorough knowledge of root canal anatomy and its variation is necessary for successful completion of root canal procedures. Morphological variations such as additional root canals in human deciduous dentition are rare. A mandibular second primary molar with more than four canals is an interesting example of anatomic variations, especially when three of these canals are located in the distal root. This case shows a rare anatomic configuration and points out the importance of looking for additional canals

Evaluation of canal transportation and centering ability of K 3 (0.02%) and K 3 (0.04%) with hand K files in primary teeth using spiral computed tomography

Background: One of the objectives of root canal preparation is to clean and shape the root canal system while maintaining the original configuration. Therefore, it is important to keep the instruments centered to provide a correct enlargement, without excessive weakening of the root structure. Aim: The aim of the study was to compare canal transportation and centering ability of K 3 (0.02% taper) and K 3 (0.04% taper) with stainless steel hand K files in primary teeth using a spiral computed tomography (SCT) scan. Materials and Methods: A total of 75 extracted mandibular primary second molars was collected. Canals were divided randomly into three groups of 25 teeth each. Group I: K 3 files (0.02% taper), Group II: K 3 (0.04% taper), and Group III: Stainless steel hand K files. Three regions from apical, mid-root, and coronal levels of the canal were recorded. All the teeth were scanned before and after instrumentation by using SCT. Results: K 3 (0.02% taper) showed less canal transportation and a better centering ratio than K 3 (0.04% taper) and stainless steel hand K files. Statistical Analysis: The mean values were compared between different study groups and the P-value was calculated by using Kruskal-Wallis one-way ANOVA. The Mann-Whitney U-test followed by the Bonferroni correction method was employed to identify the significant groups at the 5% level. Conclusion: K 3 (0.02% taper) shaped root canals without significant shaping errors when compared to K 3 (0.04% taper) and stainless steel hand K files in primary teeth