On the presence of sulphate in pituitary lutropin

The presence of sulphate in the carbohydrate of pituitary lutropin from different species has been investigated using a biosynthetic approach. Pituitaries from rats, rabbits, goats, and buffaloes were incubated in the presence of 35SO4- and the 35SO4--labelled proteins in the tissue immunoprecipitated with a well characterized anti-sheep lutropin serum. The incorporation into immunoreactive lutropin was low in the case of rat, rabbit and goat pituitaries while, it was considerable in the case of buffalo pituitaries. Hence further characterization studies were carried out on 35SO4--labelled proteins of buffaloes. The physico-chemical, immunological and biological properties of radio-labelled buffalo pituitary material were shown to be similar to those of standard lutropin. Inin vitro conditions of incubations, most of the incorporation of 35SO4- was observed into tissue lutropin while under similar conditions of incubation, [14C]-amino acids were found to get incorporated mostly into medium lutropin. The physiologically specific releasing hormone, lutropin-releasing hormone was found to stimulate the release of 35SO4--labelled lutropin from the rabbit pituitaries into the medium. These results give indirect evidence that sulphate could be present in pituitary lutropin

Multifunctional ZnO nanorod-reduced graphene oxide hybrids nanocomposites for effective water remediation: Effective sunlight driven degradation of organic dyes and rapid heavy metal adsorption

We demonstrate the multi-functionality engineering on nanocomposite by combining one dimensional (1D) ZnO nanorod (NR) and two dimensional (2D) reduced graphene oxide (rGO) for efficient water remediation. Nano-engineered ZnO NR-rGO nanocomposites show efficient water remediation in terms of degradation of organic dyes and removal of heavy metal ions. Herein, we report on the fabrication of ZnO NR-rGO nanocomposite via a facile template-free hydrothermal route with an aim to improve the visible photocatalytic efficiency of the ZnO NR based nanocomposites. The structural and morphological features reveal that the rGO sheets are attached on the ZnO NRs and form a hybrid composite assembly. The surface enabled ZnO NR-rGO nanocomposites were used to degrade organic dye molecules (methylene blue (MB), methyl orange (MO) and rhodamine B (RhB)) under visible irradiation and adsorb Cu (II) and Co (II) ions from water through an adsorption process. The nanocomposite containing 7.5 wt% rGO and ZnO NRs shows a 4-fold enhancement in the visible photocatalytic activity and effective removal of Cu (II) and Co (II) ions from aqueous solution respectively. The photocatalytic performance is discussed in detail with respect to interaction between ZnO NRs and rGO sheets, light-harvesting properties of the nanocomposites. The effective experimental adsorption data also fit very well with the pseudo-second-order model which reveals the surface adsorption of metal ions. The results provide insight into a new method utilize for both visible photo degradation and adsorption for the removal of various wastewater pollutants. Construction of hybrid form of nanostructures delivers the effective catalytic properties with tunable functionalities for the water remediation. © 2017 Elsevier B.V

Development of Textile Laminates for Improved Cut Resistance

Mechanical properties of fibres viz. tensile modulus, tenacity, elongation are the key performance indicators of cut resistance besides yarn and fabric structure. p-aramid and UHDPE (Ultra High Density Polyethylene) based high performance fibres are most commonly used for protection against mechanical risks. Specially engineered composite yarns and fabrics would help enhance cut resistance. This paper discusses on the influence textile structure configuration on the performance of cut resistant textiles. A three tier laminate composite was made using knitted Kevlar fabric (p-aramid) as outer surface, Polyurethane foam in the middle and a knitted nylon fabric as skin contact layer. This specially engineered laminate showed a 20% increase in cut resistance force when compared with the Kevlar fabric used for lamination. The combination of breathable PU foam and knitted structure of fabric yielded high stretch with improved breathability and dexterity

Heterogeneity in buffalo lutropin

Lutropin (LH-1) from water buffaloes has been shown to exhibit microheterogeneity in the N-terminal amino-acid sequence of its alpha-subunit. The beta-subunit did not exhibit such microheterogeneity. Another protocol of purification yielded a preparation of buffalo LH (bu LH-2) different from the buffalo LH-1 in certain physico-chemical properties like ease of dissociation into subunits, sugar composition, isoelectric point, and elution profile on S-200. Data appear to indicate the presence of more than one form of buffalo lutropin

Applying multi-response optimization for sustainable machining of 316 stainless steel with coconut oil assisted minimum quantity lubrication

Environmental machining was investigated using coconut oil and minimal quantity lubrication (MQL) in the turning of AISI 316 stainless steel. The turning parameters and MQL flow rate were optimized using ANOM and ANOVA in multi-response analysis to produce the best hardness and minimum surface roughness in the machined surface of AISI 316 stainless steel. The feed, speed, depth of cut, and MQL flow rate during turning were used as the input parameters and surface roughness and hardness as the output parameters. The experimental plan was developed using Taguchi's L9 orthogonal array. It was found that minimum surface roughness (Ra: 1.12 µm and Rz: 6.37 µm) was achieved at a cutting speed of 120 m/min, feed rate between 0.25 to 0.3 mm/rev, the depth of cut between 1.0 to 1.5 mm and a MQL flow rate of 90 ml/hr. Micro hardness was measured from the machined surface to a depth of 1.075 mm to determine the machining affected zone (MAZ). It has been noted that the hardness reduced with an increase in machined surface depth. The MQL with coconut oil was shown to be an ecofriendly lubrication method for machining difficult-to-cut materials like stainless steel and keeping good surface integrity

Volumetric and optical properties of ACE inhibitor captopril in aqueous-alcoholic mixtures

Captopril is an angiotensin-converting enzyme (ACE) inhibitor that is used for the treatment of hypertension and congestive heart failure. This article addresses the accurate measurements of densities and refractive indices of solutions containing captopril in pure solvents such as water, methanol, ethanol and 1-propanol and aqueous mixtures of methanol, ethanol and propan-1-ol of 30%, 50% and 70% by volume in a wide range of drug concentration at 26 °C. This article also includes the evaluation of apparent molar volume, partial molar volume at infinite dilution and transfer volumes. The concentration dependence of the refractive indices studied and respective fitting parameters have been reported. Different properties are interpreted in terms of intermolecular interactions, effect of drug on structure of solvent/solvent mixture and overall structural fittings in solutions